Fungi Present in Antarctic Deep-Sea Sediments Assessed Using DNA Metabarcoding

Ogaki, Mayara Baptistucci; Pinto, Otávio Henrique Bezerra; Vieira, Rosemary; Neto, Arthur Ayres; Convey, Peter; Carvalho‐Silva, Micheline; Rosa, Carlos A.; Câmara, Paulo Eduardo Aguiar Saraiva; Rosa, Luiz Henrique

doi:10.1007/s00248-020-01658-8

Cited by 31 publications

(31 citation statements)

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“…Microbial assemblages in benthic deep-sea ecosystems play an important role in C and nutrient cycling and transfer of energy and material to the higher trophic levels [11]. Besides prokaryotes, fungi are widespread in deep-sea environments spanning from hypersaline anoxic basins [12][13][14] to cold seeps [15,16], from hydrothermal vents [17][18][19] to surface and subsurface sediments [13,[20][21][22][23][24], including benthic Antarctic systems [25][26][27][28] and references therein). Theoretical estimates suggest that fungi can be the most diversified component of eukaryotes on Earth, with more than 5 million species of which only 5% have been described [29,30].…”

Section: Introductionmentioning

confidence: 99%

Local Environmental Conditions Promote High Turnover Diversity of Benthic Deep-Sea Fungi in the Ross Sea (Antarctica)

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Corinaldesi

Rastelli

et al. 2022

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Fungi are a ubiquitous component of marine systems, but their quantitative relevance, biodiversity and ecological role in benthic deep-sea ecosystems remain largely unexplored. In this study, we investigated fungal abundance, diversity and assemblage composition in two benthic deep-sea sites of the Ross Sea (Southern Ocean, Antarctica), characterized by different environmental conditions (i.e., temperature, salinity, trophic availability). Our results indicate that fungal abundance (estimated as the number of 18S rDNA copies g−1) varied by almost one order of magnitude between the two benthic sites, consistently with changes in sediment characteristics and trophic availability. The highest fungal richness (in terms of Amplicon Sequence Variants−ASVs) was encountered in the sediments characterized by the highest organic matter content, indicating potential control of trophic availability on fungal diversity. The composition of fungal assemblages was highly diverse between sites and within each site (similarity less than 10%), suggesting that differences in environmental and ecological characteristics occurring even at a small spatial scale can promote high turnover diversity. Overall, this study provides new insights on the factors influencing the abundance and diversity of benthic deep-sea fungi inhabiting the Ross Sea, and also paves the way for a better understanding of the potential responses of benthic deep-sea fungi inhabiting Antarctic ecosystems in light of current and future climate changes.

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Section: Introductionmentioning

confidence: 99%

Local Environmental Conditions Promote High Turnover Diversity of Benthic Deep-Sea Fungi in the Ross Sea (Antarctica)

Barone

Corinaldesi

Rastelli

et al. 2022

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“…The internal transcribed spacer 2 (ITS2) of the nuclear ribosomal DNA was used as a DNA barcode for molecular species identification (Chen et al 2010, Richardson et al 2015). ITS2 has been widely used in barcoding studies of many organisms (Ruppert et al 2009) and has proved effective in recent studies of Antarctic diversity (Câmara et al 2020, Rosa et al 2020, Ogaki et al 2021). PCR amplicons were generated using the universal primers ITS3 and ITS4 (White et al 1990) and were sequenced by high-throughput sequencing (HTS) at Macrogen, Inc. (South Korea) on an Illumina MiSeq sequencer (3 × 300 bp) using the MiSeq Reagent Kit v3 (600-cycle) following the manufacturer's protocol.…”

Section: Methodsmentioning

confidence: 99%

“…The use of DNA metabarcoding approaches provides an effective method for the detection of rare species (Ruppert et al 2019, Câmara et al 2020). However, as yet, few studies have applied metabarcoding approaches in studies of Antarctic ecosystems (but see Fraser et al 2018, Câmara et al 2020, Garrido-Benavent et al 2020, Rosa et al 2020, Ogaki et al 2021). Rippin et al (2018) reported that this approach revealed ~11 times greater richness than a traditional morphological approach in a study on Livingston Island, South Shetland Islands.…”

Section: Introductionmentioning

confidence: 99%

Periphyton diversity in two different Antarctic lakes assessed using metabarcoding

et al. 2021

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Antarctic lakes have generally simple periphyton communities when compared with those of lower latitudes. To date, assessment of microbial diversity in Antarctica has relied heavily on traditional direct observation and cultivation methods. In this study, sterilized cotton baits were left submerged for two years in two lakes on King George Island and Deception Island, South Shetland Islands (Maritime Antarctic), followed by assessment of diversity by metabarcoding using high-throughput sequencing. DNA sequences of 44 taxa belonging to four kingdoms and seven phyla were found. Thirty-six taxa were detected in Hennequin Lake on King George Island and 20 taxa were detected in Soto Lake on Deception Island. However, no significant difference in species composition was detected between the two assemblages (Shannon index). Our data suggest that metabarcoding provides a suitable method for the assessment of periphyton biodiversity in oligotrophic Antarctic lakes.

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“…Costa Coelho et al, 2021), shallow-water and deep-sea sediments (Vieira et al, 2018;Wentzel et al, 2019;Ogaki et al, 2020a;Ogaki et al, 2021), macroalgae (Godinho et al, 2013;Furbino et al, 2014;Furbino et al, 2018), sponges (Henríquez et al, 2014), and lakes (Gonçalves et al, 2012;Ogaki et al, 2020b). These results suggest that the Antarctic environments could constitute important reservoirs for new species of Pseudogymnoascus not yet described.…”

Section: Introductionmentioning

confidence: 92%

Description of the First Four Species of the Genus Pseudogymnoascus From Antarctica

et al. 2021

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The genus Pseudogymnoascus represents a diverse group of fungi widely distributed in different cold regions on Earth. Our current knowledge of the species of Pseudogymnoascus is still very limited. Currently, there are only 15 accepted species of Pseudogymnoascus that have been isolated from different environments in the Northern Hemisphere. In contrast, species of Pseudogymnoascus from the Southern Hemisphere have not yet been described. In this work, we characterized four fungal strains obtained from Antarctic marine sponges. Based on multilocus phylogenetic analyses and morphological characterizations we determined that these strains are new species, for which the names Pseudogymnoascus antarcticus sp. nov., Pseudogymnoascus australis sp. nov., Pseudogymnoascus griseus sp. nov., and Pseudogymnoascus lanuginosus sp. nov. are proposed. Phylogenetic analyses indicate that the new species form distinct lineages separated from other species of Pseudogymnoascus with strong support. The new species do not form sexual structures and differ from the currently known species mainly in the shape and size of their conidia, the presence of chains of arthroconidia, and the appearance of their colonies. This is the first report of new species of Pseudogymnoascus not only from Antarctica but also from the Southern Hemisphere.

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Fungi Present in Antarctic Deep-Sea Sediments Assessed Using DNA Metabarcoding

Cited by 31 publications

References 68 publications

Local Environmental Conditions Promote High Turnover Diversity of Benthic Deep-Sea Fungi in the Ross Sea (Antarctica)

Local Environmental Conditions Promote High Turnover Diversity of Benthic Deep-Sea Fungi in the Ross Sea (Antarctica)

Periphyton diversity in two different Antarctic lakes assessed using metabarcoding

Description of the First Four Species of the Genus Pseudogymnoascus From Antarctica

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