Fungitoxicity of natural heterocycle glucoside vicine obtained from Vicia faba L. against selectedmicroscopic filamentous fungi

Abstract

Vicine and convicine were isolated from mature seeds of Vicia faba L. These compounds were separated from the relevant fractions by HPLC and identified by IR, MS and NMR. Obtained vicine was used for all bioassays. Vicine in concentration of 25 µg in 1 ml of medium had significant toxic effect. The concentration of 5 µg of vicine in 1 ml of medium had significant fungistatic effect for fungi Fusarium culmorum and Alternaria alternata. The concentration of 500 ng of vicine in 1 ml of medium had significant fungistatic effect for fungi Cladosporium herbarum and Botrytis cinerea. The effective concentration of vicine in our test was significantly lower than is the concentration of vicine in seeds and pods of V. faba. The toxicity of vicine comparing with the toxicity of insecticide Actellic was higher by one order. Also, the fungicide Impact had a lower toxicity than vicine. Fungitoxic and fungistatic effect of vicine was comparable with some other tested fungicides and with the plant saponin digitonin. Our results confirmed that vicine is important natural fungicide.

UHPLC‐ESI‐QTOF‐MS‐based metabolic profiling of Vicia faba L. (Fabaceae) seeds as a key strategy for characterization in foodomics

“…The known pyrimidine glucosides, vicine and convicine, widely spread in Fabaceae, including V. faba , and two novel isomers ( 14 – 17 ) were found by the proposed methodology. These isomers could have been underestimated by previous methodologies due to the use of stationary phases with higher particle size and UV detection . The aglycone divicine ([C 4 H 6 N 4 O 2 ‒ H] − ) at m / z 141.0418, the aglycone isouramil ([C 4 H 5 N 3 O 3 ‒ H] − ) at m / z 142.037, and their odd ions were detected in MS 2 spectra of vicine and convicine, respectively, after the loss of hexose moieties.…”

Potential of Fava Bean as Future Protein Supply to Partially Replace Meat Intake in the Human Diet

“…Large variations in vicine and convicine contents are observed among different fava bean cultivars and genotypes, with low levels of pyrimidine glycosides in varieties already present on the market (Hussein and others ). From an agronomic perspective, vicine and convicine are part of plant mechanisms of resistance toward some phytopathogenic fungi and exerts natural fungicide activity higher than some common insecticides (Pavlik and others ). Vicine is also the main mortality factor for Callosobruchus maculatus larvae and for other phytophagous pests (Desroches and others ).…”

Fine‐mapping of the major locus for vicine and convicine in faba bean (Vicia faba) and marker‐assisted breeding of a novel, low vicine and convicine winter faba bean population

“…Although there are hints that VC may confer antifungal effects (Bjerg et al, 1984; Pavlík et al, 2002), there were no agronomic disadvantages of LVC material detected in accompanying field trials (Abo‐Vici Consortium, 2021); moreover, data are lacking on the actual VC content in roots and shoots of HVC and LVC types, be it spring or winter types of faba bean.…”

“…Although there are hints that VC may confer antifungal effects (Bjerg et al, 1984;Pavlík et al, 2002), there were no agronomic disad-…”