Two lignan glucosides, the known tracheloside and the new carthamoside, together with their two known aglycones, trachelogenin and (7E,8'S)-7,8-didehydroarctigenin (cf. cartha- mogenin), were isolated from the seeds of Leuzea carthamoides. Additional four phenylpropanoid derivatives of tryptamine, the E and Z isomers of N-feruloylserotonin and N-isoferuloylserotonin, were also isolated and identified. Their structure determination and identification were performed by spectroscopic, mainly NMR analysis.
Vicine and convicine were isolated from mature seeds of Vicia faba L. These compounds were separated from the relevant fractions by HPLC and identified by IR, MS and NMR. Obtained vicine was used for all bioassays. Vicine in concentration of 25 µg in 1 ml of medium had significant toxic effect. The concentration of 5 µg of vicine in 1 ml of medium had significant fungistatic effect for fungi Fusarium culmorum and Alternaria alternata. The concentration of 500 ng of vicine in 1 ml of medium had significant fungistatic effect for fungi Cladosporium herbarum and Botrytis cinerea. The effective concentration of vicine in our test was significantly lower than is the concentration of vicine in seeds and pods of V. faba. The toxicity of vicine comparing with the toxicity of insecticide Actellic was higher by one order. Also, the fungicide Impact had a lower toxicity than vicine. Fungitoxic and fungistatic effect of vicine was comparable with some other tested fungicides and with the plant saponin digitonin. Our results confirmed that vicine is important natural fungicide.
From the root of Senecio nemorensis L. var.subdecurens Girsb. ex Pantocs. a mixture of the following compounds was isolated: sesquiterpenic hydrocarbons of mass 204 and paraffinic hydrocarbons C11 - C13, a secondary paraffinic alcohol - ginnol a mixture of triterpenic alcohols bauerenol and lupeol, a mixture of caretenoids, a mixture of phytosterols, i.e. β-sitosterol, stigmasterol, D-aromadendren-4-ol (spathulenol) (I), D-aromadendrane-4β,10α-diol (III), and nemosenin E (1β,10β-epoxy-3β-hydroxy-6β-isovaleryloxyfuroeremophilane (X).
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