Fungos endofíticos de Eugenia dysenterica DC como biocontroladores de fitopatógenos in vitro

Abstract

Os micro-organismos antagonistas (bactérias, leveduras e fungos filamentosos) têm a capacidade de exercer um efeito antagonista sobre diferentes patógenos sendo empregados para controlar diversas enfermidades de frutos e vegetais. Este estudo objetivou isolar e testar microrganismos presentes em Eugenia dysenterica DC contra Aspergillus parasiticus, Colletotrichum gloeosporioides e Monilinia fructicola e in vitro. Foram coletados caules e folhas de 30 indivíduos de Cagaiteira. Os fragmentos vegetais passaram por desinfecção superficial, de acordo com o proposto por Endophytic fungi Eugenia dysenterica DC as biocontrol of plant pathogens in vitro.ABSTRACT-The antagonistic microorganisms (bacteria, yeasts and filamentous fungi) are able to exert an antagonistic effect on various pathogens being used to control various diseases of fruits and vegetables. This study aimed to isolate and test microorganisms present in Eugenia dysenterica DC against Aspergillus parasiticus, Colletotrichum gloeosporioides, and Monilinia fructicola and in vitro. Stems and leaves were collected from 30 specimens of cagaiteira. The samples were submited to a surface disinfection, according to the proposed by Rose, et. al, (2010). Fragments were inoculated on Petri plates containing the agar Potato 25-28 ° C for 60 days. 263 endophytic fungi strains were obtained, purified and assessed for their potential in to produce inhibitore volatile or diffusible substances. A total of 96 isolates showed antagonistic activity against plant pathogens. 45 isolates being able to inhibit the growth of A. parasiticus. Against the pathogen C. gloeosporioides 68 endophytes showed antagonist activity. Against the pathogen M. fructicola only 17 fungi were able to exert inhibitory effect. These isolates showed potential for the production of bioactive substances against these pathogens. Index terms: Eugenia dysenterica DC, antagonism, endophytic, phytopathogensCopyright: © 2015 JBFS all rights. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Financiamento: Os autores reportam que não houve suporte e auxílio financeiro. Conflito de interesse:Os autores declaram que não há conflito de interesse. Como referir esse documento (ABNT):MALTA, C. M.; FERREIRA, E. M. S.; COELHO, C. M.; PIMENTA, R. S. Fungos endofíticos de Eugenia dysenterica DC como biocontroladores de fitopatógenos in vitro.