Fur‐Regulated Genes in <i>Coxiella burnetii</i>

Briggs, Heather L.; Wilson, Marvin C.; Seshadri, Rekha; Samuel, James E.

doi:10.1196/annals.1355.008

Cited by 1 publication

(3 citation statements)

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“…We recently reported the identification of feoB (CBU1766), a ferrous iron transporter gene, in the genome annotation and the initial characterization of C. burnetii Fur regulation of the gene (11). FeoB has been previously described as a Fur-regulated ferrous iron acquisition protein in E. coli (34).…”

Section: Vol 76 2008 Role Of Iron-regulated Genes In C Burnetii Pamentioning

confidence: 99%

“…While feoB (CBU1766), frg1 (CBU0970), CBU0216, CBU1106, and CBU0968 were predicted as being transcriptionally regulated by iron, the acid activation buffer system did not evaluate Fur regulation. To indirectly assess C. burnetii Fur interaction with the putative Fur boxes, a heterologous expression system was developed by using a two-plasmid regulator/promoter system in an E. coli fur mutant (11). This method also allowed for an indirect comparison of regulatory activities of C. burnetii Fur and E. coli Fur.…”

Section: Vol 76 2008 Role Of Iron-regulated Genes In C Burnetii Pamentioning

confidence: 99%

“…We previously identified 18 putative Fur-regulated genes by an in silico approach (11). Briefly, the C. burnetii genome was searched using the E. coli Fur box consensus sequence and the putative Fur boxes of feoB and frg1.…”

Section: Vol 76 2008 Role Of Iron-regulated Genes In C Burnetii Pamentioning

confidence: 99%

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Limited Role for Iron Regulation in Coxiella burnetii Pathogenesis

Briggs

Pul

Seshadri³

et al. 2008

Infect Immun

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In gram-negative bacteria, iron acquisition proteins are commonly regulated by Fur (ferric uptake regulator), which binds iron-regulated promoters (the Fur box). We hypothesized that Coxiella burnetii requires iron and employs an iron-regulatory system and used various approaches to define a Fur regulon. Cloned C. burnetii fur complemented an Escherichia coli fur deletion mutant. A ferrous iron transporter gene (CBU1766), a putative iron binding protein-encoding gene (CBU0970), and a cation efflux pump gene (CBU1362) were identified by genome annotation and using a Fur titration assay. Bioinformatically predicted Fur box-containing promoters were tested for transcriptional control by iron. Five genes demonstrated at least a twofold induction with minimal iron. Putatively regulated genes were evaluated in a two-plasmid regulator/promoter heterologous expression system. These data suggested a very limited Fur-regulated system in C. burnetii. In an in vitro tissue culture model, a significant increase in bacterial growth was observed with infected cells treated with deferoxamine in comparison to growth under iron-replete conditions. In an iron-overloaded animal model in vivo, the level of bacterial growth detected in the iron-injected animals was significantly decreased in comparison to growth in control animals. In a low-iron-diet animal model, a significant increase in splenomegaly was observed, but no significant change in bacterial growth was identified. The small number of predicted iron acquisition systems, few Fur-regulated genes, and enhanced replication under a decreased iron level predict a requirement of a low level of iron for survival, perhaps to avoid creation of additional reactive oxygen radicals.

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Section: Vol 76 2008 Role Of Iron-regulated Genes In C Burnetii Pamentioning

confidence: 99%

Section: Vol 76 2008 Role Of Iron-regulated Genes In C Burnetii Pamentioning

confidence: 99%