FurA modulates gene expression of alr3808, a DpsA homologue in Nostoc (Anabaena) sp. PCC7120

Abstract

The DNA-binding protein from stationary phase (Dps) protein family plays an important role in protecting microorganisms from oxidative and nutritional stresses. In silico analysis of the promoter region of alr3808, a dpsA homologue from the cyanobacterium Nostoc sp. PCC7120 shows putative ironboxes with high homology with those recognized by FurA (ferric uptake regulator). Evidence for the modulation of dpsA by FurA was obtained using in vitro and in vivo approaches. SELEX linked to PCR was used to identify P dpsA as a FurA target. Concurrently, EMSA assays showed high affinity of FurA for the dpsA promoter region. DpsA expression analysis in an insertional mutant of the alr1690-afurA message (that exhibited an increased expression of FurA) showed a reduced synthesis of DpsA. These studies suggest that FurA plays a significant role in the regulation of the DpsA.

Ferric Uptake Regulator (FUR) protein: properties and implications in cyanobacteria

“…In Anabaena sp. PCC 7120, prxA and dpsA contain multiple AT-rich regions that partially match with the FurB (Zur) consensus sequence described previously (Napolitano et al 2012), and are modulated by additional regulatory factors (Hernandez et al 2007;Yingping et al 2014), as also reported in the case of heterotrophic bacteria (Kallifidas et al 2009). …”

Cross-talk Between Iron and Nitrogen Regulatory Networks in Anabaena (Nostoc) sp. PCC 7120: Identification of Overlapping Genes in FurA and NtcA Regulons

“…However, we failed to detect binding of NtcA to the dpsA UPSR even if this DNA-binding protein is overexpressed under nitrogen starvation. 35,39 Dual recognition of the xisA and hanA UPSRs, whose expression is required at the first stages of heterocyst differentiation, 40,41 was also detected by EMSA. Brusca et al 42 identified a regulatory region upstream xisA that blocks its expression in vegetative cells.…”

Identification and biochemical properties of Dps (starvation‐induced DNA binding protein) from cyanobacterium Anabaena sp. PCC 7120

“…Recent research showed that DpsA (alr3808), a hemecontaining member of Dps family (22), which receives regulation from Ferric Uptake Regulator (FurA) (23), also exists in Anabaena sp. PCC 7120.…”