Furanone at Subinhibitory Concentrations Enhances Staphylococcal Biofilm Formation by
            <i>luxS</i>
            Repression

Kuehl, Richard; Al‐Bataineh, Sameer A.; Gordon, Oliver; Luginbuehl, Reto; Otto, Michael; Textor, Marcus; Landmann, Régine

doi:10.1128/aac.01704-08

Cited by 88 publications

(76 citation statements)

References 44 publications

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“…Likewise, synthetic furanones and furanone analogues incorporating thiophenone moieties decreased staphylococcal biofilm formation mediated by AI-2 (245,253). However, these reports are in conflict with findings that luxS mutants of Staphylococcus epidermidis display increased biofilm formation (254) and that furanones supplied at sub-MICs actually enhance biofilm development (255). It will be difficult to resolve these apparently contradictory studies without further investigations; however, in agreement among them were findings that furanone concentrations of above 10 M were cytotoxic, and since the strongest biological activities were seen near these concentrations, perhaps their effects involve nonspecific stress responses in addition to AI-2 responses.…”

Section: Natural-product Qs Inhibitorscontrasting

confidence: 54%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

689

556

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SUMMARY Cell-cell communication, or quorum sensing, is a widespread phenomenon in bacteria that is used to coordinate gene expression among local populations. Its use by bacterial pathogens to regulate genes that promote invasion, defense, and spread has been particularly well documented. With the ongoing emergence of antibiotic-resistant pathogens, there is a current need for development of alternative therapeutic strategies. An antivirulence approach by which quorum sensing is impeded has caught on as a viable means to manipulate bacterial processes, especially pathogenic traits that are harmful to human and animal health and agricultural productivity. The identification and development of chemical compounds and enzymes that facilitate quorum-sensing inhibition (QSI) by targeting signaling molecules, signal biogenesis, or signal detection are reviewed here. Overall, the evidence suggests that QSI therapy may be efficacious against some, but not necessarily all, bacterial pathogens, and several failures and ongoing concerns that may steer future studies in productive directions are discussed. Nevertheless, various QSI successes have rightfully perpetuated excitement surrounding new potential therapies, and this review highlights promising QSI leads in disrupting pathogenesis in both plants and animals.

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Section: Natural-product Qs Inhibitorscontrasting

confidence: 54%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

689

556

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“…To this aim, LuxS was incubated with cold ATP in the presence of Stk1 and subjected to mass spectrometry analysis after tryptic digestion. The sequence coverage of the protein was complete, and phosphorylation occurred on only one peptide (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22), as illustrated by the tandem mass spectrometry (MS-MS) spectra, unambiguously confirming the presence of one phosphate group on Thr14 ( Fig. 2A).…”

mentioning

confidence: 58%

“…The LuxS/AI-2 system in Staphylococcus aureus, a highly adaptable Gram-positive bacterium responsible for numerous clinical infections, has been analyzed in detail (13,14,28). Moreover, the emergence of antibiotic resistance has become a serious concern, especially due to methicillin-resistant S. aureus (MRSA) isolates that are resistant to all available penicillins and other ␤-lactam antimicrobial drugs (7).…”

mentioning

confidence: 99%

The Staphylococcus aureus Autoinducer-2 Synthase LuxS Is Regulated by Ser/Thr Phosphorylation

et al. 2010

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The Staphylococcus aureus autoinducer-2 (AI-2) producer protein LuxS is phosphorylated by the Ser/Thr kinase Stk1 at a unique position, Thr14. The enzymatic activity of the phosphorylated isoform of LuxS was abrogated compared to that of nonphosphorylated LuxS, thus providing the first evidence of an AI-2-producing enzyme regulated by phosphorylation and demonstrating that S. aureus possesses an original and specific system for controlling AI-2 synthesis.

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“…Halogenated furanone compounds have been considered to be potentially ideal antibacterial agents on implants for preventing biofilm infection due to their broad spectrum antimicrobial activity, 20,21 novel mechanisms of antimicrobial action, 22 and lack of toxicity and genotoxic effects. 25,26 The BBF used in this study is a member of the synthetic halogenated furanone family of compounds.…”

Section: Discussionmentioning

confidence: 99%

“…These compounds have been demonstrated to have potent antimicrobial activity against a great number of Grampositive and Gram-negative bacteria and to inhibit biofilm formation. 20,21 Halogenated furanone compounds can control multicellular behavior in various microbial species induced by autoinducer-1 and autoinducer-2, 22 which are used for cell-to-cell communication to control production and secretion of virulence factors, proteolytic activity, carbohydrate metabolism, and biofilm formation. 23 The novel nature of their mechanisms of action would allow these new antimicrobials to be effective against bacteria that are currently antibiotic-resistant.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and in vitro release behavior of a novel antibacterial coating containing halogenated furanone-loaded poly(L-lactic acid) nanoparticles on microarc-oxidized titanium

Cheng¹,

Wu²,

Gao³

et al. 2012

IJN

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Background: Dental implants have become increasingly common for the management of missing teeth. However, peri-implant infection remains a problem, is usually difficult to treat, and may lead eventually to dental implant failure. The aim of this study was to fabricate a novel antibacterial coating containing a halogenated furanone compound, ie, (Z-)-4-bromo-5-(bromomethylene)-2(5H)-furanone (BBF)-loaded poly(L-lactic acid) (PLLA) nanoparticles on microarc-oxidized titanium and to evaluate its release behavior in vitro. Methods: BBF-loaded PLLA nanoparticles were prepared using the emulsion solventevaporation method, and the antibacterial coating was fabricated by cross-linking BBF-loaded PLLA nanoparticles with gelatin on microarc-oxidized titanium. Results: The BBF-loaded PLLA nanoparticles had a small particle size (408 ± 14 nm), a low polydispersity index (0.140 ± 0.008), a high encapsulation efficiency (72.44% ± 1.27%), and a fine spherical shape with a smooth surface. The morphology of the fabricated antibacterial coating showed that the BBF-loaded PLLA nanoparticles were well distributed in the pores of the microarc oxidation coating, and were cross-linked with each other and the wall pores by gelatin. The release study indicated that the antibacterial coating could achieve sustained release of BBF for 60 days, with a slight initial burst release during the first 4 hours. Conclusion:The novel antibacterial coating fabricated in this study is a potentially promising method for prevention of early peri-implant infection.

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Furanone at Subinhibitory Concentrations Enhances Staphylococcal Biofilm Formation by luxS Repression

Cited by 88 publications

References 44 publications

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

The Staphylococcus aureus Autoinducer-2 Synthase LuxS Is Regulated by Ser/Thr Phosphorylation

Fabrication and in vitro release behavior of a novel antibacterial coating containing halogenated furanone-loaded poly(L-lactic acid) nanoparticles on microarc-oxidized titanium

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