1990
DOI: 10.1159/000235115
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Further Characterization of Surface Membrane Structures Expressed on Human Basophils and Mast Cells

Abstract: Recently, we were able to establish the immunologic surface marker profile of human basophils and mast cells. In the present study, the characterization of these cell types was extended by the use of monoclonal antibodies (mAbs) to hemopoietic differentiation antigens. Basophils and mast cells were enriched by mAbs and complement from chronic myeloid leukemia blood (n = 5) and dispersed lung tissue (n = 4), respectively. A panel of 80 mAbs was tested for being reactive with purified cell populations using flow… Show more

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Cited by 101 publications
(72 citation statements)
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“…The phenotype analysis was performed on a FACScan analyser (Becton-Dickinson). The results ob tained are described in table 2 and are in accordance with those published by Valent et al [23] concerning the sur face membrane stuctures expressed on human circulating basophils, except for the presence of HLA-DR. HLA-DR was present on about 45% of our purified basophils and, since this phenotype analysis was performed on 4 samples originating from different cultured bone marrow precur sor cells, this discrepancy may reflect different states of Fig. 2.…”
supporting
confidence: 89%
“…The phenotype analysis was performed on a FACScan analyser (Becton-Dickinson). The results ob tained are described in table 2 and are in accordance with those published by Valent et al [23] concerning the sur face membrane stuctures expressed on human circulating basophils, except for the presence of HLA-DR. HLA-DR was present on about 45% of our purified basophils and, since this phenotype analysis was performed on 4 samples originating from different cultured bone marrow precur sor cells, this discrepancy may reflect different states of Fig. 2.…”
supporting
confidence: 89%
“…Expression of the IgG receptor on basophils was demonstrated using radio-labeled agglgG by Ishizaka et al [33] for the first time. Later, this IgG receptor on basophils was shown to be FcyRlI/CDw32 [10][11][12][13]. Here, we show that stimulation by anti-FcyRll mAb does not provoke histamine release from human basophils.…”
Section: Discussionmentioning
confidence: 52%
“…On contact with allergen, recruited baso phils are activated through a cross-linking of surface bound IgE molecules and high-affinity IgE receptors, FceRI, lead ing to biochemical signaling events and chemical mediator release [6][7][8][9], Besides FceRI, human basophils also express a number of surface molecules and receptors including Fc receptors K Ä R G E R ' 1996 S. Karger AG. Basel l \ W I \ V J C I \ 1018 2438/96/1091 -0027 $8.00/0 H-Mail karger«/ kargcr.ch Fax +41 61 306 12 34 forlgG [10][11][12][13], Based on the reactivity o f monoclonal anti bodies (rnAbs) and molecular cloning, three different IgG receptors, namely FcyRI (CD64), FcyRll (CDw32) and FcyRIll (CD16) have been identified to date [6.14.15]. Each of them has distinct isoforms that show cell-type-specific expression.…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…CD40 is a member of the nerve growth factor/TNF receptor superfamily (9)(10)(11). CD40 signaling can be initiated by cross-linking CD40 with anti-CD40 mAb or by interaction with CD40 ligand (CD40L), now referred to as gp39, which is expressed by CD4 ϩ T cells (12)(13)(14) and, as recently shown, by human mast cells and basophils (15,16). The importance of CD40-CD40L interactions in vivo has been recently demonstrated in autoimmune diseases such as collagen-induced arthritis (17), acute and chronic graft-versushost disease (18), and lupus nephritis (19) where treatment of experimental animals with anti-gp39 antibodies markedly prevented disease expression.…”
Section: Introductionmentioning
confidence: 99%