Further Effects of Nonsteroidal Anti-inflammatory Compounds on Blastocyst Hatching in vitro and Implantation Rates in the Mouse1

Hurst, Philip; Macfarlane, D.

doi:10.1095/biolreprod25.4.777

Cited by 35 publications

(21 citation statements)

References 17 publications

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“…It was confirmed for the first time that morphological changes from 2-cell embryos until blastocoel formation and the time needed for such development did not differ between parthenogenetic and fertilized embryos. In the fertilized embryos of the present study, it was also confirmed that morphological changes until blastocoel formation and the diameters of the blastocyst stage are consistent with those of previous reports with fertilized mouse embryos [14][15][16][17].…”

Section: Discussionsupporting

confidence: 92%

Time-Lapse Videomicrographic Observations of Parthenogenetic Mouse Embryos during Early Development.

Niimura

Futatsumata

1999

J.Mamm.Ova.Res.

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Abstract:The process of early development in diploid parthenogenetic mouse embryos from the 2-cell to the blastocyst stages was observed by time-lapse videomicrography, and was compared with that in fertilized embryos. Parthenogenetic 2-cell embryos cleaved and developed to 8-cell embryos after 23.0 hrs of culture. Transformation of blastomeres occurred at the 8-cell stage: namely, outer blastomeres were flattened. The embryos compacted at the morula stage, and then developed to blastocysts after 54.0 hrs of culture. A slit in the zona pellucida was formed 42.7 hrs after blastocyst formation, and trophectoderm cells protruded out of the zona pellucida through the slit. Protrusion of trophectoderm cells from the zona pellucida could arise from either side, polar trophectoderm or mural trophectoderm. After 7.0 hrs, the blastocysts completely escaped from the zona pellucida in either state, expansion or contraction. Fertilized 2-cell embryos showed morphological changes similar to those of parthenogenetic embryos and developed to blastocysts after 51.9 hrs of culture. Fertilized blastocysts took a significantly shorter time, 31.1 hrs, to start hatching, but required a significantly longer time, 23.8 hrs, to complete hatching, compared with parthenogenetic blastocysts. Hatching patterns of fertilized blastocysts were consistent with those of parthenogenetic blastocysts, except that hatching began with protrusion of trophectoderm cells from small holes in zonae pellucidae of fertilized blastocysts. From these results, it was confirmed that the developmental ability of early diploid parthenogenetic embryos prepared by the treatment with ethanol and cytochalasin B is comparable to that in fertilized embryos.

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Section: Discussionsupporting

confidence: 92%

Time-Lapse Videomicrographic Observations of Parthenogenetic Mouse Embryos during Early Development.

Niimura

Futatsumata

1999

J.Mamm.Ova.Res.

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“…It was also determined that the number of weak contractions until 32 h after blastocoel formation was similar in zona-intact and zona-free mouse blastocysts, whereas strong contractions were observed more in zona-free blastocysts (Table 5) [33]. Mouse blastocysts treated with indomethacin (IM), which is an inhibitor of the biosynthesis of prostaglandins (PGs) related to blastocyst hatching [34,35], had the same number of weak contractions as that observed in non-treated blastocysts, but the number of strong contractions was larger and the hatching rate was lower in the IM-treated blastocysts [36]. When blastocysts were treated with PGF2α or PGE2, in combination with IM, the hatching rate and the number of weak and strong contractions were comparable to those observed in non-treated blastocysts (Table 6) [36].…”

Section: Role Of Contractions In Blastocyst Hatchingmentioning

confidence: 99%

Time-Lapse Videomicrographic Analyses of Contractions in Mouse Blastocysts

Niimura

2003

J. Reprod. Dev.

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Abstract. Contraction has been observed in cultured blastocysts of many mammals, but little is known about the features of the contraction and its physiological role in blastocysts. The author analyzed contractions of a large number of cultured mouse blastocysts by time-lapse videomicrography. The results revealed that blastocysts repeated contractions of different degrees during the expanded stage from 10 h after blastocoel formation, and that the number of contractions was greater during the hatching period than in the periods pre-and post-hatching. The results also showed that the time needed for both contraction and re-expansion to the size before contraction tended to lengthen in blastocysts severely contracted. It was inferred that contractions of blastocysts occur physiologically in relation to myosin light chain kinase, but not due to an increase in permeability between trophectoderm cells in association with their division, or the influence of culture. Furthermore, it was inferred that re-expansion of contracted blastocysts occurs due to active transport and accumulation of Na + from the trophectoderm cells into blastocoelic fluid as a result of the action of Na + /K + -ATPase activated in the membrane of trophectoderm cells. Our results suggested that contractions are also present in blastocysts developed in vivo, and that weak contractions (less than 20% volume reduction) play an important role in hatching, whereas strong contractions (20% or more volume reduction) have the effect of inhibiting hatching. From our results on contractions of various blastocysts, it seems possible to evaluate the developmental ability of embryos, i.e. embryo quality, based on contractions of blastocysts.

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“…Blastocoelic expansion has been reported as prostaglandin-dependent since several nonsteroidal anti-inflammatory drugs, including indomethacin and meclofenamic acid, and inhibitory analogues of prostaglandins E and F-2rx limit expansion and reduce hatching (Biggers, Leonov, Baskar & Fried, 1978;Baskar et ai, 1981;Hurst & MacFarlane, 1981). Weakening or lysis of the zona pellucida is believed to be due to a zona lysin of uterine origin which may also have a role in initiating implantation (McLaren, 1970;Mintz, 1972;Hoversland & Weitlauf, 1981, 1982.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of hatching and trophoblastic outgrowth by mouse embryos cultured in Whitten's medium containing plasmin and plasminogen

Menino¹,

O'Claray²

1986

Reproduction

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Summary. Mice were induced to superovulate and 2-cell embryos were cultured in Whitten's medium with 10 mg bovine serum albumin/ml (WM) as control, Medium WM with 2\m=.\3,4\m=.\6,23\m=.\1 or 46\m=.\2\ g=m\ gplasmin/ml, Medium WM with 14\m=.\6,29\m=.\1 or 145\m=.\7 \ g = m \ g plasminogen/ml, Medium WM with 0\m=.\1,0\m=.\2, 1\m=.\1or 2\m=.\2 \g=m\gtrypsin/ml, Medium WM with 0\m=.\2,0\m=.\3, 1\m=.\6or 3\m=.\3 \g=m\gpronase/ml and Medium WM with 10% heat-treated bovine serum (HTBS). Proteolytic activities in the culture media were evaluated at the start of the culture period and 10 days later. Blastocyst formation was significantly reduced in cultures supplemented with pronase and in the two higher levels of trypsin when compared to that in Medium WM. More embryos developed to the blastocyst stage in Medium WM + 2\m=.\3 or 23\m=.\1\ g=m\ gplasmin/ml and Medium WM + 14\m=.\6\ g=m\ gplasminogen/ ml than in Medium WM (P < 0\m=.\05). The incidence of hatching was significantly greater in Medium WM than in all plasminogen-and plasmin-supplemented media except for Medium WM + 29\m=.\1 \g=m\gplasminogen/ml. Although not significantly different, hatching was lower in Medium WM and Medium WM + 0\m=.\1 \g=m\gtrypsin/ml when compared to Medium WM + HTBS. Similar numbers of embryos completed the hatching process in Media WM, WM + 0\m=.\1 or 0\ m=. \ 2\ g=m\ gtrypsin/ml and WM + 0\m=.\3 \ g = m \ g pronase/ml. Since dissolution of the zona pellucida occurred within 96 h for embryos cultured in Media WM + 1\ m=. \ 6 or 3\ m=. \ 3\ g=m\gpronase/ml and WM + 1\ m=. \ 1 or 2\ m=. \ 2\ g=m\gtrypsin/ ml, hatching could not be evaluated. The incidence of attachment and subsequent trophoblastic outgrowth was significantly greater in media with plasmin, plasminogen and HTBS than in pronase-and trypsin-supplemented media or in Medium WM alone. The results suggest that the enhancement in embryo development observed in pl asmi n\x=req-\ and plasminogen-supplemented media is due not only to the provision of protease but to an additional trophic effect as well. Simple medium supplemented with plasmin or plasminogen is as able to support in-vitro development as is medium supplemented with serum.

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Further Effects of Nonsteroidal Anti-inflammatory Compounds on Blastocyst Hatching in vitro and Implantation Rates in the Mouse1

Abstract: ABSTRACT

Cited by 35 publications

References 17 publications

Time-Lapse Videomicrographic Observations of Parthenogenetic Mouse Embryos during Early Development.

Time-Lapse Videomicrographic Observations of Parthenogenetic Mouse Embryos during Early Development.

Time-Lapse Videomicrographic Analyses of Contractions in Mouse Blastocysts

Enhancement of hatching and trophoblastic outgrowth by mouse embryos cultured in Whitten's medium containing plasmin and plasminogen

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