Further Evidence for the Cross-Bridging Hypothesis for Flocculation of Brewer's Yeast

Lyons, T. P.; Hough, J. S.

doi:10.1002/j.2050-0416.1971.tb06950.x

Cited by 56 publications

(15 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This absorption did not depend on the presence of an energy source and may well be due to non-specific binding to the yeast cell wall (this presumably was also the case with the "petitepositive" species in the absence of an energy source). Yeast cell walls carry a number of negatively charged groups (Lyons and Hough, 1971) and it is reasonable to propose that acriflavine (a cation) will bind ionically to such a structure.…”

Section: Methodsmentioning

confidence: 99%

A possible relationship between the fatty acid composition of yeasts and the ‘petite’ mutation

Johnson

Brown

1972

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

A possible relationship between the fatty acid composition of yeasts and the ‘petite’ mutation

Johnson

Brown

1972

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

“…Although they found that esterification of carboxyl groups in the wall with 1,2-epoxypropane caused a drop of only 20 yo in the calcium-binding capacity of the wall, they failed to find a correlation between the amounts of acidic amino-acid residues in the walls and the flocculating ability of organisms from which they were derived. Lyons & Hough (1971) concluded that the capacity of strains of S. cerevisiae to flocculate is proportional to the density of phosphate groups in the phosphomannan-protein layer of the wall.…”

Section: Introductionmentioning

confidence: 99%

Role of Wall Phosphomannan in Flocculation of Saccharomyces cerevisiae

Jayatissa

Rose

1976

Journal of General Microbiology

View full text Add to dashboard Cite

S U M M A R YTreatment with 60 yo hydrofluoric acid (HF) removed most of the phosphorus and small amounts of mannan, glucan and protein from walls of two nonflocculent strains ( N C Y C~~~ and NCYCI004) and two flocculent strains (NCYCIOOS and NCYC I 063) of Saccharornyces cerevisiae. Organisms of all strains showed increased flocculating ability following H F treatment. Flocculation of untreated organisms of NCYCIOOS and N C Y C I O~~, and of HF-treated organisms of all four strains, declined appreciably when they were washed in deionized water, with or without EDTA, and the flocculation was measured in deionized water instead of in 0.05 M-sodium acetate containing Ca2 +. Treatment with I ,a-epoxypropane also caused a decrease in the flocculating ability of these organisms. Extracting the lipids from organisms of strains N C Y C~~~ and N C Y C I O O~ had no effect on their flocculating ability, but decreased the flocculating ability of organisms of strains NCYCIOO5 and N C Y C I O~~. pH-electrophoretic mobility curves of untreated and HF-treated organisms confirmed the loss of wall phosphate by H F treatment, and indicated that HF treatment had little effect on the content of protein carboxyl groups in the outer wall layers. Mannose at 0 . 2 2~ completely prevented floc formation by organisms of strain N C Y C I O~~ ; but, even at 0.33 M, it had very little effect on floc formation by HF-treated organisms of strains ~c u c 3 6 6 and ~c u c r o 6 3 . Organisms of all four strains bound fluorescein-conjugated concanavalin A to the same extent after treatment with HF as before, but this treatment led to a greatly diminished binding of fluorescein-conjugated antiserum raised against organisms of strain N C Y C~~~. The results indicate that phosphodiester linkages in yeast-wall mannan are not involved in bridge formation through Ca2 + during floc formation and that this arises principally through carboxyl groups.

show abstract

“…Previous studies concerning differences in the structure of the cell walls between flocculent and non-flocculent yeast were normally conducted on isolated cell wall material (14,15). However, isolation of cell walls involves mechanical disruption of cells (20) which may lead not only to structural alterations in the wall but also to adsorption of intracellular material to the cell wall.…”

Section: Discussionmentioning

confidence: 99%

“…This indicates that the phenomenon is restricted to the surface of cell envelopes and does not involve cytoplasmic materials. The phosphomannan-protein complex appears to be responsible for this behaviour of the isolated cell wall because its removal by alkali (12), digestion with trypsin (14,15) or digestion with papain (8) decreases flocculation.…”

Section: Introductionmentioning

confidence: 99%

Studies on yeast flocculation. Comparison of enzymatic digests of flocculent and non-flocculent cells of Saccharomyces carlsbergensis

Marfey

Sørensen

Ottesen

1977

Carlsberg Res. Commun.

View full text Add to dashboard Cite

Treatment of a flocculent (strain 201) and a non-flocculent (strain 116) cell suspension of Saccharomyces carlsbergensis with trypsin, followed by 13-mercaptoethanol and a second trypsin treatment solubilized approximately 15% of material from both types of cells based on dry weight. The solubilized fractions were resolved by Sephadex gel filtration into high mol.wt. (40,000 to 200,000) and low mol.wt. (less than 4,000) components. High mol.wt, components from the flocculent cells contained more carbohydrate and less protein than the corresponding components isolated from the non-flocculent cells. Furthermore, the polypeptide material from the flocculent cells had a higher content of proline, lysine, and arginine than that from non-flocculent cells. High mol.wt, material contained antigenic determinants similar to those previously found in beer fractions. Yeast-derived antigens were found in all fractions while antigens of barley origin were found only in the tryptic digests.A differential radioactive labelling technique demonstrated minor differences between the components released from flocculent and non-flocculent cell residues upon digestion with pronase.

show abstract

Further Evidence for the Cross-Bridging Hypothesis for Flocculation of Brewer's Yeast

Cited by 56 publications

References 10 publications

A possible relationship between the fatty acid composition of yeasts and the ‘petite’ mutation

A possible relationship between the fatty acid composition of yeasts and the ‘petite’ mutation

Role of Wall Phosphomannan in Flocculation of Saccharomyces cerevisiae

Studies on yeast flocculation. Comparison of enzymatic digests of flocculent and non-flocculent cells of Saccharomyces carlsbergensis

Contact Info

Product

Resources

About