Further Evidence for Two Modes of Hypothermia Damage

Kruuv, J.; Glofcheski, D.J.

doi:10.1006/cryo.1993.1030

Cited by 12 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sperm samples were obtained individually from three males by injecting of the synthetic analog of gonadotropin releasing hormone [10]. The sperm samples were placed on ice and transported to the laboratory.…”

Section: Sperm Collectionmentioning

confidence: 99%

See 1 more Smart Citation

Preliminary results on ascorbic acid and lysine suppression of clastogenic effect of deep-frozen sperm of the Russian sturgeon (Acipenser gueldenstaedti)

Mirzoyan

Nebesikhina

Voynova

et al. 2006

International Journal of Refrigeration

View full text Add to dashboard Cite

Section: Sperm Collectionmentioning

confidence: 99%

“…The commonly used for sturgeon sperm cryopresevation cryoprotectant dimethylsulfoxide (DMSO) is a known hydroxyl-free radical producer [9]. It has been reported to be ineffective in protecting V-79 Chinese hamster cells exposed to hypothermia [10]. DMSO is also known for its pharmacological toxicity.…”

Section: Introductionmentioning

confidence: 99%

Preliminary results on ascorbic acid and lysine suppression of clastogenic effect of deep-frozen sperm of the Russian sturgeon (Acipenser gueldenstaedti)

Mirzoyan

Nebesikhina

Voynova

et al. 2006

International Journal of Refrigeration

View full text Add to dashboard Cite

“…Though DMSO is a scavenger of hydroxyl free radical, it is ineffective at protecting V-79 Chinese hamster cells exposed to hypothermia (Kruuv & Glofcheski 1993). DMSO also has pharmacological toxicity.…”

Section: Introductionmentioning

confidence: 99%

Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol

et al. 2005

View full text Add to dashboard Cite

Green tea polyphenol (GTP) together with dimethylsulphoxide (DMSO) were added to a freezing solution of osteoblastic cells (rat calvarial osteoblasts and human osteosarcoma cells) exposed to repeated freeze/thaw cycles (FTC) to induce oxidative stress. When cells were subjected to 3 FTCs, freezing medium containing 10% (v/v) DMSO and 500 mug GTP ml(-1) significantly (p<0.05) suppressed cell detachment and growth inhibition by over 63% and protected cell morphology. Furthermore, the alkaline phosphatase activity of osteoblastic cells was appreciably maintained after 2 and 3 FTCs in this mixture. Polyphenols may thus be of use as a cell cryopreservant and be advantageous in such fields as cell transplantation and tissue engineering.

show abstract

“…In this respect, it is quite interesting how histologically similar hyperfiltration defects in native kidneys with a reduced number of functioning nephrons are to the lesions found in kidneys [13] with chronic transplant failure [31]. The impact of hypothermia on renal cell viability is not fully understood, but there is clear evidence that even mild hypothermia can seriously damage nonischemic cells [17,181. Some mechanisms underlying the impairment of hypothermic function appear to be identical to defects due to normothermic hypoxia.…”

Section: Introductionmentioning

confidence: 99%

Nonfreezing cryopreservation – a possible means of improving long-term transplant function?

1998

View full text Add to dashboard Cite

Improving organ preservation techniques for transplantation is one of the most important goals of transplantation research. We have established a new, nonfreezing cryopreservation method to optimize the viability of rat kidneys for transplantation with up to 4 M dimethylsulphoxide (DMSO) in EuroCollins solution (EC) at -5 degrees C to -15 degrees C. We have confirmed the occurrence of a tubular and glomerular defect pattern that mediates acute tubular necrosis (ATN) and that may be a cause of major histocompatibility complex (MHC) independent immunological components of chronic transplant rejection. The extent of this defect [transplant survival and function, 31P-NMR spectroscopy, histological defect index] in the nonfreezing cryopreserved groups (n = 22) is significantly (P = 0.017) lower than in the simple cold storage group (n = 12). Quality and localization of the lesions in kidney transplants can elucidate the context of organ preservation, progressive hyperfiltration defects, and the occurrence of graft failure without elevated frequency of acute rejection episodes. These results indicate that further efforts to provide higher pretransplant organ viability without using it to prolong cold storage intervals may provide better insight into MHC-independent factors of chronic transplant failure and may result in improved long-term transplant outcome.

show abstract

Further Evidence for Two Modes of Hypothermia Damage

Cited by 12 publications

References 0 publications

Preliminary results on ascorbic acid and lysine suppression of clastogenic effect of deep-frozen sperm of the Russian sturgeon (Acipenser gueldenstaedti)

Preliminary results on ascorbic acid and lysine suppression of clastogenic effect of deep-frozen sperm of the Russian sturgeon (Acipenser gueldenstaedti)

Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol

Nonfreezing cryopreservation – a possible means of improving long-term transplant function?

Contact Info

Product

Resources

About