Further evidence for very virulent infectious bursal disease virus in vaccinated chickens in Nigeria

Owolodun, Olajide Adewale; Yakubu, Bitrus; Jambol, Anvou; Audu, B. J.; Dogonyaro, B. B.; Luka, Pam Dachung

doi:10.1007/s11250-015-0880-x

Cited by 12 publications

(13 citation statements)

References 14 publications

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“…Comparison of the amino acid sequences of the viruses used in this study with previously published Nigerian IBDV isolates showed some similarity [20]. The only exceptions were substitution mutations seen at amino acid position T219 and S269 in some of the viruses used in this study but not seen in previously published Nigerian IBDV [18-21]. These mutations suggest that vvIBDV strains are continuing to evolve in Nigeria.…”

Section: Discussionsupporting

confidence: 49%

Molecular characterization of field infectious bursal disease virus isolates from Nigeria

Nwagbo¹,

Shittu

Nwosuh

et al. 2016

Vet World

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Aim:To characterize field isolates of infectious bursal disease virus (IBDV) from outbreaks in nine states in Nigeria through reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis of portions of the VP2 and VP1 genes and to determine the presence or absence of reassortant viruses.Materials and Methods:A total of 377 bursa samples were collected from 201 suspected IBD outbreaks during 2009 to 2014 from nine states in Nigeria. Samples were subjected to RT-PCR using VP2 and VP1 gene specific primers, and the resulting PCR products were sequenced.Results:A total of 143 samples were positive for IBDV by RT-PCR. These assays amplified a 743 bp fragment from nt 701 to 1444 in the IBDV VP2 hypervariable region (hvVP2) of segment A and a 722 bp fragment from nt 168 to 889 in the VP1 gene of segment B. RT-PCR products were sequenced, aligned and compared with reference IBDV sequences obtained from GenBank. All but one hvVP2 sequence showed similarity to very virulent IBDV (vvIBDV) reference strains, yet only 3 of the VP1 67 VP1 sequences showed similarity to the VP1 gene of vvIBDV. Phylogenetic analysis revealed a new lineage of Nigerian reassortant IBDV strains.Conclusion:Phylogenetic analysis of partial sequences of genome segment A and B of IBDV in Nigeria confirmed the existence of vvIBDV in Nigeria. In addition, we noted the existence of reassortant IBDV strains with novel triplet amino acid motifs at positions 145, 146 and 147 in the reassorted Nigerian IBDV.

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Section: Discussionsupporting

confidence: 49%

Molecular characterization of field infectious bursal disease virus isolates from Nigeria

Nwagbo¹,

Shittu

Nwosuh

et al. 2016

Vet World

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“…Phylogenetic analysis indicates that IBDV/VOM/NG/2017 is a vvIBDV strain due to its clustering with reference vvIBDVs used for comparison. The vvIBDV strains have been reported in vaccinated and unvaccinated poultry flocks in some parts of Nigeria [10,11,19]. For ease of classification and identification of the probable origin and relatedness of some vvIBDVs from Africa, African vvIBDVs are divided into three; VV-1, consists of IBDV from Ethiopia, Nigeria and Zambia, VV-2, consists of IBDV from Nigeria, Tanzania and Zambia, while VV-3, collectively consists of IBDV from Africa, Asia, Europe and some other countries [20].…”

Section: Discussionmentioning

confidence: 99%

“…These IBDVs have been generally considered atypical isolates that evolved in restricted geographic regions or during a short period of time under particular conditions. In Nigeria, IBD was first reported in 1973 and since then despite routine vaccination programme, IBDV has assumed an endemic status with vvIBDV being reported throughout the country [10,11,12]. An accurate identification of the field variants circulating in poultry production is essential to understand the epidemiology and control of the disease [13].…”

Section: Introductionmentioning

confidence: 99%

Genetic Analysis of Infectious Bursal Disease Virus (IBDV) from a Farm in Vom, Plateau State, Nigeria

Enurah¹,

Nwosuh²,

Ehizibolo³

et al. 2018

JAMB

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This study describes the genetic analysis of an atypical infectious bursal disease virus (IBDV) detected from an outbreak in a poultry farm in Vom, Plateau State, Nigeria in 2017. The farm with seven thousand, six hundred and eleven (7,611) four weeks old vaccinated pullets had 76.2% mortality (5,796 dead birds) as a result of the outbreak. Thirty-two bursae of Fabricius samples showing lesions of IBD were aseptically collected for IBDV antigen detection and molecular characterisation. All the tested samples were positive by the agar-gel immunodiffusion test (AGDT) for IBDV antigen while the VP2 gene could be detected in 60% of these samples by polymerase chain reaction (PCR). One PCR positive sample was sequenced. Nucleotide and deduced amino acid sequences obtained were compared to sequences from GenBank. The IBDV strain detected (IBDV/VOM/NG/2017) was distinct from the attenuated vaccine strain used on the farm, but formed cluster in the very virulent (vv) IBDV group in the VV2-1 subcluster and was most closely related to previously published IBDV strains from Nigeria (Plateau 104/NG/2010, and more recently Original Research Article

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“…Since it was first described in Nigeria in 1973 (Ojo et al, 1973), IBD has ravaged and still continues to wreck havoc on the poultry industry in Nigeria, which has made it achieve an endemic status. There have been several reports of outbreaks of IBD in both vaccinated and unvaccinated poultry chickens in Nigeria (Adamu et al, 2013;Luka et al, 2014;Owolodun et al, 2015;Nwagbo et al, 2016). Previous and recent studies have implicated very virulent (vv) strains of IBD as being responsible for these outbreaks (Owoade et al, 2004;Adamu et al, 2013;Luka et al, 2014;Owolodun et al, 2015;Nwagbo et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…There have been several reports of outbreaks of IBD in both vaccinated and unvaccinated poultry chickens in Nigeria (Adamu et al, 2013;Luka et al, 2014;Owolodun et al, 2015;Nwagbo et al, 2016). Previous and recent studies have implicated very virulent (vv) strains of IBD as being responsible for these outbreaks (Owoade et al, 2004;Adamu et al, 2013;Luka et al, 2014;Owolodun et al, 2015;Nwagbo et al, 2016). In addition, a very recent study also implicated reassortant IBDV strains as contributing to the failure of IBD vaccine in protecting chickens against field IBDV infection in Nigeria (Nwagbo et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Continuous circulation and mutation at the hypervariable region of the VP2 gene of very virulent infectious bursal disease virus in Nigeria

Nwagbo¹,

Shittu²,

Qasim³

et al. 2018

Nig. Vet. J.

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Infectious bursal disease (IBD) is a highly contagious viral disease of poultry with huge economic consequences. First reported in Nigeria in 1973, the disease has since become endemic across the country. Inspite of routine vaccine administration, outbreaks are frequently reported in vaccinated flocks which questions the effectiveness of the vaccine to confer adequate protection against the circulating strains of the virus. In this study, suspected IBD outbreaks from 9 poultry farms spanning 2015-2016 were investigated. The hypervariable region (HVR) of the VP2 gene of infectious bursal disease virus (IBDV) from these outbreaks was sequenced. Based on the phylogenetic tree and deduced amino acid sequences, the viruses clustered with African very virulent (vv) types (VV2-1) with markers typical for vvIBDV at positions 222A, 242I, 256I, 294I and 299S. Also, unique substitutions were identified at the hydrophilic peak A (215Q→P) and minor peak 2 (280N→Y) in addition to that previously unreported at position 308I→L. These results demonstrate continuous circulation and mutations at the HVR of the VP2 which underscores the need for constant monitoring of IBDV in Nigerian poultry. Further study is required to establish the biological function(s) of these mutations on the pathogenicity and antigenicity of the virus.

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Further evidence for very virulent infectious bursal disease virus in vaccinated chickens in Nigeria

Cited by 12 publications

References 14 publications

Molecular characterization of field infectious bursal disease virus isolates from Nigeria

Molecular characterization of field infectious bursal disease virus isolates from Nigeria

Genetic Analysis of Infectious Bursal Disease Virus (IBDV) from a Farm in Vom, Plateau State, Nigeria

Continuous circulation and mutation at the hypervariable region of the VP2 gene of very virulent infectious bursal disease virus in Nigeria

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