Peste‐des‐petits‐ruminants (PPR) and Goat pox (GTP) are two devastating and economically important transboundary animal diseases of small ruminants in Africa and Asia that have been difficult to control. This study however, investigated an outbreak of PPR and GTP in a mixed flock of indigenous sheep and goats in Kanam, North Central Nigeria. A total of nine sera and seven tissues (lungs, spleen, scab and skin) samples were collected and analysed in the laboratory using competitive enzyme linked immunosorbent assay (cELISA) for PPR antibodies and polymerase chain reaction (PCR) for detection of PPR virus (PPRV) and GTP virus (GTPV). Gene fragments of the nucleoprotein of PPRV and the G‐protein‐coupled chemokine receptor (GPCR) of GTPV were amplified and sequenced to confirm the presence of the causative viruses. Serologically, antibodies to PPRV were detected in all (9/9) sera collected. GTPV and PPRV was detected in corresponding samples (42.8% n = 3/7) of the scab/skin samples collected by both PCR and RT‐PCR technique. The phylogenetic analysis of PPRV revealed that the virus belongs to lineage IV and clustered with viruses from Gabon and Cameroon. Similarly, the GTPV also clustered with other sequences from Burkina Faso and Yemen. The positive cELISA, RT‐PCR and PCR results from samples collected from the same animals confirmed co‐infection of PPR and GTP in this mixed flock of sheep and goats. This is the first report of concurrent infection of PPR and GTP in mixed flock of sheep and goats in Nigeria. Our findings underscore the need for farmers to vaccinate their flock to control spread and economic losses as result of these diseases.

Contagious ecthyma (CE) is a debilitating disease of sheep, goats and other ruminants caused by Orf virus (ORFV). Suspected outbreaks of CE were reported in three flocks of goats in Jos-South, Plateau State, Nigeria with proliferative lesions on the muzzle, oral commissures, perineal area and legs. Scab samples were collected from all the flocks and the affected animals placed on antibiotics. The samples collected were homogenized and the DNA extracted using QIAamp® DNA Mini kit (QIAGEN, Hilden Germany). The extracted DNA was subjected to polymerase chain reaction (PCR) amplifying two gene fragments: A32L and B2L of the ORFV. Two flocks were West African Dwarf (WAD) breed of goats with100% morbidity and 100% mortality recorded, while the third flock was Kano Brown breed with 6.7% morbidity and no mortality. A32L and B2L fragments of the ORFV genome was amplified by PCR from samples collected from the flocks. Contagious ecthyma was therefore confirmed based on classical clinical presentations and laboratory confirmation by PCR. Amplification of A32L and B2L gene fragments of the ORFV and 100% mortality in WAD breed of goats is the first report in Nigeria associated with CE. Further studies should be carried out to understand the role of breed, the epidemiology and economic impact of CE in Nigeria for the utilization of an appropriate control strategy.

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Reverse transcription polymerase chain reaction (RT-PCR) and partial sequencing of the VP2 hypervariable region was performed on clinical samples from two infectious bursal disease (IBD) outbreaks in Plateau state, Nigeria. IBD virus RNA was detected in all four bursa of Fabricius samples. Nucleotide sequencing and analysis of the four samples revealed high similarity to previous IBDV sequences from northern and southern Nigeria. The deduced amino acid sequences were compared to reference IBDV strains retrieved from the GenBank; virulence markers A222, I256, and I294 were conserved in both outbreak and reference sequences. Amino acid residue S254 was conserved in the outbreak viruses and previous viruses from northern Nigeria. Phylogenetic analysis revealed that all four viruses were very virulent IBDVs. These viruses clustered with vv2-1 variant viruses from Oyo and Ogun states and less closely with vv2-2 isolates from Tanzania. The nucleotide identity of the sequences in this study ranged from 99.6 to 100 % with each other. These findings are further evidence of IBD outbreaks in vaccinated chicken flocks in Nigeria.

Aim: Characterization of Infectious bursal disease viruses (IBDV) from the two outbreaks in Jos Nigeria, using reverse transcriptase polymerase chain reaction/restriction fragment length polymorphism (RT-PCR/RFLP) technique. Materials and Methods:A total of 40 bursa samples were collected from two outbreaks in November 2011 from two farms of 6-8 weeks old pullets within Jos South Local Government Area, with mortality between 60 -74.2 % in commercially reared layer chicken flocks experiencing signs typical of infectious bursal disease (IBD). All the samples were found to contain IBDV genome by One Step RT-PCR using VP2 gene specific primers. Result:The assay amplified a 743bp fragment from 701-1444 nucleotides. RT-PCR product was further subjected to restriction digestion using TaqI, MvaI and SacI Restriction enzymes to differentiate classical from very virulent phenotypes. The RFLP profile was found similar for all eight isolates with TaqI and MvaI enzyme but different for SacI. All eight TaqIpositive Viruses were further found positive for MvaI digestion and yielded RFLP profile similar to vvIBDV in Europe whereas one isolate was SacI positive and had a RFLP profile similar to classic IBDV strains. Conclusion:The clinical history of high mortality and TaqI and MvaI restriction enzyme positivity revealed that vvIBDV strains still exist in Jos, North central Nigeria.