Further Immunohistochemical Study of Crooke's Hyalin

Uei, Yoshio; Kanzaki, Masaki; Yabana, Tadashi

doi:10.1016/s0344-0338(11)80137-8

Cited by 8 publications

(6 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In studies of pituitary tissues from autopsy of patients who received sustained treatment with glucocorticoids, Uei and Suzuki found a significant correlation with the presence of Crooke's changes and 1) the total dose of glucocorticoids and 2) the duration of treatment (7). However, others did not find such a correlation (8).…”

Section: Discussionmentioning

confidence: 96%

Crooke's Changes In Cushing's Syndrome Depends on Degree of Hypercortisolism and Individual Susceptibility

Oldfield

Vance²,

Louis

et al. 2015

The Journal of Clinical Endocrinology &Amp; Metabolism

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 96%

Crooke's Changes In Cushing's Syndrome Depends on Degree of Hypercortisolism and Individual Susceptibility

Oldfield

Vance²,

Louis

et al. 2015

The Journal of Clinical Endocrinology &Amp; Metabolism

View full text Add to dashboard Cite

show abstract

“…Cytokeratin markers have become a useful tool in diagnostic pathology, as different cytokeratin subtypes are specific to particular epithelial cells and their associated neoplasms. Although the immunohistochemistry profile of cytokeratin subsets in the normal pituitary gland is not well characterized, immunoreactivity for CK8 and CK18 is well described [7,9,14].…”

Section: Discussionmentioning

confidence: 99%

“…However, they are not always readily identified on routine H&E-stained sections. Although immunoreactivity for cytokeratin filaments has been documented in Crooke's cells [3][4][5][6][7][8] and positivity for CK8 and CK18 has been reported [7,9], these cytokeratin subsets are not specific for Crooke's cells because many in diagnostic pathology, primarily in ascertaining the primary origin of many types of metastatic adenocarcinomas. In 1992, Moll et al [10] reported that the cells of the pituitary gland were negative for CK20 expression, an observation confirmed in our recent study [13].…”

Section: Discussionmentioning

confidence: 99%

“…The Crooke's cell is now recognized as a nonneoplastic corticotroph cell that is often found in patients with primary hypercortisolism, including those with adrenocorticotrophic hormone consist of intermediate filaments that correspond to type I filaments and are consistent with those of the cytokeratin type [3][4][5][6][7]. These intermediate filaments demonstrate immunopositivity when stained with antibodies against low-molecularweight cytokeratins, including CK8 and CK18 (CAM 5.2) [7]. However, ACTH adenomas, and even normal pituitary endocrine cells, exhibit CAM 5.2 positivity when stained with these antibodies [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…To date, the classic Crooke's cell has been identified partially based on the cell's characteristic microscopic appearance with routine H&E stains and partially by immunopositivity for CAM 5.2 [7,14]. However, the presence of the classic Crooke's cell is not a sensitive marker for either endogenous or exogenous hypercortisolism, and little has been reported about the cytoskeletal changes that occur within the corticotroph cell in response to hypercortisolism.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cytokeratin CK20 is a sensitive marker for Crooke's cells and the early cytoskeletal changes associated with hypercortisolism within pituitary corticotrophs

Eschbacher¹,

Coons²

2006

Endocr Pathol

View full text Add to dashboard Cite

Crooke's cells are nonneoplastic corticotroph cells found in the adenohypophysis of patients who have an endogenous or exogenous excess of glucocorticoids. Classic Crooke's cells have a prominent hyaline cytoplasmic ring that displaces the basophilic granules of the normal cell. This characteristic appearance is produced by a perinuclear accumulation of cytokeratin filaments. Immunohistochemistry for cytokeratins is a sensitive way to identify Crooke's cells, but a keratin antibody specific for Crooke's hyaline change has not been reported. Normal pituitary epithelial cells are variably reactive for many keratin antibodies but are negative for cytokeratin 20 (CK20) expression. We evaluated the use of CK20 immunohistochemistry as a marker for Crooke's cells. We examined sections from 25 pituitary glands resected from 15 patients who had undergone exogenous glucocorticoid administration and from 10 patients with an endogenous source of hypercortisolism; sections from 10 normal pituitary glands obtained at autopsy were used as controls. CK20 immunoreactivity was observed only in corticotrophs. A staining pattern consistent with classic Crooke's cells was seen in pituitary gland sections from 15 of the cases. Cells with less intense CK20 positivity were present in sections from all 25 cases. We found CK20 to be a sensitive and specific marker for Crooke's cells and also for the previously unrecognized, subtle, cytoskeletal changes that occur in corticotrophs in response to hypercortisolism.

show abstract

Cushing’s syndrome caused by an ectopic pituitary adenoma of the sphenoid sinus: Adrenal crisis after partial resections of the adenoma

et al. 1994

View full text Add to dashboard Cite

Further Immunohistochemical Study of Crooke's Hyalin

Cited by 8 publications

References 1 publication

Crooke's Changes In Cushing's Syndrome Depends on Degree of Hypercortisolism and Individual Susceptibility

Crooke's Changes In Cushing's Syndrome Depends on Degree of Hypercortisolism and Individual Susceptibility

Cytokeratin CK20 is a sensitive marker for Crooke's cells and the early cytoskeletal changes associated with hypercortisolism within pituitary corticotrophs

Cushing’s syndrome caused by an ectopic pituitary adenoma of the sphenoid sinus: Adrenal crisis after partial resections of the adenoma

Contact Info

Product

Resources

About