Further in vivo studies on the biosynthetic relationship between the major brain gangliosides in rat

Biodegradation of the major rabbit retinal gangliosides, studied in vivo

“…The sialidase labile sialic acid of the ganglioside mixture GMl, GDla, GDlb and GTl had a higher specific activity than the sialidase stable sialic acid, up to 4 weeks after the precursor injection. This is in agreement with the results of the previous short-term experiments on retina [S] and brain [9,10].…”

“…The sialidase labile sialic acid of the ganglioside mixture GMl, GDla, GDlb and GTl had a higher specific activity than the sialidase stable sialic acid, up to 4 weeks after the precursor injection. This is in agreement with the results of the previous short-term experiments on retina [S] and brain [9,10]. of the GD3 sialic acid decreased rapidly, with a halflife of approximately 2 weeks, in contrast to the still increasing specific activity of the sialic acids of the other gangliosides.…”

“…Approximately 100 nmol GD3 was hydrolysed with sialidase from Vibrio Cholera (Neuraminidase, Behringwerke) [9] and the liberated sialic acid was isolated from the remaining lipid on a 1 g Sephadex G-25 fine column [9].…”

“…An aliquot of the ganglioside mixture, corresponding to 100-200 nmol sialic acid, was hydrolysed with sialidase and later formic acid, and the liberated sialidase labile and stable sialic acids were isolated from the remaining lipids on 1 g Sephadex G-25 fine columns [9].…”

Subcellular biosynthesis and transport of gangliosides formed from exogenous lactosylceramide in rat liver

“…266 Time after injection (h) spectively. Conversely, in the nervous system a precursor-product relationship in the course of ganglioside biosynthesis is still controversial (Maccioni et al, 1971;Holm et al 1974;).…”