Further Investigations of Actinomycetes by Scanning Electron Microscopy

Williams, Stanley T.

doi:10.1099/00221287-62-1-67

Cited by 30 publications

(9 citation statements)

References 13 publications

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“…Furthermore, the method is adaptable and can be used to examine freeze-dried cells, untreated cells, or cells fixed with other chemicals. Other methods commonly used to examine bacterial colonies involve either excising agar blocks from agar plates containing colonies or growing bacteria on microcover slips and placing them directly onto specimen stubs selected one that would support bacterial (5,6,8,(12)(13)(14). With these methods, some growth; would be readily available, easily handifficulties arise during drying and chemical dled, and sterilizable; would withstand chemifixation.…”

Section: Discussionmentioning

confidence: 99%

Scanning Electron Microscopy of Bacterial Colonies

Afrikian

Julian

Bulla

1973

Applied Microbiology

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A technique is described for observing bacterial colony growth. Bacillus cereus, B. subtilis, and B. cereus var. mycoides were grown on strips of dialysis membrane layered on nutrient agar. Microcolonies of the organisms on strips were fixed in Formalin vapor in situ; the strips then were removed from the agar and secured to scanning microscope specimen stubs without markedly disturbing the cellular arrangement. Scanning electron micrographs clearly depict morphology of individual cells, as well as the spatial orientation of cells within the colony. This technique is reproducible, adaptable, and simple.

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Section: Discussionmentioning

confidence: 99%

Scanning Electron Microscopy of Bacterial Colonies

Afrikian

Julian

Bulla

1973

Applied Microbiology

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“…This method enables the mycelium and structures produced in the solid growth media to be studied, unlike the previous studies of the growth and micromorphology of actinomycetes, which have concentrated on the mycelial development on surfaces such as dialysis tubing (Afrikian et af., 1973), coverslips and slides (Williams & Davies, 1967;Locci, 1976) and the growth media (Williams, 1970). Fig.…”

Section: Results a N D Discussionmentioning

confidence: 99%

A Method for the Examination of the Substrate Mycelium of Actinomycetes by Scanning Electron Microscopy

Wood¹

1985

Microbiology

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“…myces vulgaris and Actinobifida dichotomica have a ridged outer coat, but internally they resemble the endospores of Bacillus and Clost r i d i u m (8,23).…”

Section: Fig 4 Coiled Chain Of Ridged Spores I N Spherical Body Afmentioning

confidence: 99%

Streptosporangium corrugation sp.nov., an Actinomycete with Some Unusual Morphological Features

Williams

Sharples

1976

International Journal of Systematic Bacteriology

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The micromorphology, ultrastructure, and cell wall composition of an actinomycete isolated from beach sand were studied. On the basis of its cell wall composition and some of its morphological features, it was placed in the genus Streptosporangium. It differed from other members of this genus by producing rather ill-defined sporangia containing thick-walled, ridged spores together with short chains of similar spores. This organism is regarded as belonging to a new species, for which we propose the name Streptosporangium corrugatum. The type strain of this species is E90 (NCIB 11120).Genera of the order Actinomycetales are distinguished primarily by their spore morphology and the chemical composition of their walls (6,11,18). Although there are differences between the various classification schemes proposed, all actinomycetes which form their spores within sporangia or vesicles and have meso-2,6-diaminopimelic acid in their walls are placed in the family Actinoplanaceae . Genera within this family can be divided into two groups (6). Those in the first group (Actinoplanes, Ampullariella, and Dactylosporangium) form sparse aerial mycelium and have glycine and meso-diaminopimelic acid in their cell walls. The second group (Planomonospora, Plano bispora, Spirillospora, and Streptosporangium) have a well developed aerial mycelium and lack glycine in their cell walls.We have examined the morphology and cell wall composition of an isolate which belongs to the family Actinoplanaceae but which has several morphological characteristics not previously observed in other members of this family. The purpose of this paper is to report the results of our studies on this isolate. MATERIALS AND METHODSBacterial strain. Strain E90 was isolated from beach sand (pH 7.8) at Freshfield, Lancashire, United Kingdom. It was detected on dilution plates prepared with starch-casein medium containing antifungal antibiotics (24). Most actinomycetes on the plates were micromonosporas (22), and E90 accounted for less than 1% of the total colonies.Cultural characteristics. Cultures were first grown on oatmeal agar at a range of temperatures to determine the temperature most suitable for subsequent experiments. Optimum growth occurred at 25 C.The isolate was then grown on a variety of media (see Table 1) for 21 days. The extent of growth of substrate and aerial mycelium and presence of sporing structures were noted. Colors of aerial growth, substrate growth, and soluble pigments were determined using the color chart of Prauser (17).Morphology and fine structure. Most aerial growth and spore formation occurred on oatmeal agar, and this medium was used for studies of morphology. Observations by light microscopy were carried out on blocks of medium removed from plate cultures after 7, 14, and 21 days of incubation.For scanning electron microscopy, small blocks of medium with growth were removed from plates after the same periods, and these were quenched in isopentane at about -150 C and then freeze dried at -60 C in a Pearce Edwards tissue dryer for ...

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Further Investigations of Actinomycetes by Scanning Electron Microscopy

Cited by 30 publications

References 13 publications

Scanning Electron Microscopy of Bacterial Colonies

Scanning Electron Microscopy of Bacterial Colonies

A Method for the Examination of the Substrate Mycelium of Actinomycetes by Scanning Electron Microscopy

Streptosporangium corrugation sp.nov., an Actinomycete with Some Unusual Morphological Features

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