Further Observations on the Morphogenesis of the Round Headed Human Spermatozoa

Baccetti, Baccio; Renieri, Tommaso; Rosati, Floriana; Selmi, Maria Gloria; Casanova, S.

doi:10.1111/j.1439-0272.1977.tb01298.x

Cited by 51 publications

(19 citation statements)

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“…We propose that the abnormal membranous structures and ectopic acrosomes observed during spermiogenesis represent failed Golgi attempts of acrosome development. This interpretation is at variance with that of other authors that described these features as regressive changes of the acrosome (Holstein et al, 1973;Anton-Lamprecht et al, 1976;Baccetti et al, 1977Baccetti et al, , 1991Nistal and Paniagua, 1978;Holstein and Schirren, 1979).…”

Section: Discussioncontrasting

confidence: 55%

Acrosomal biogenesis in human globozoospermia: immunocytochemical, ultrastructural and proteomic studies

2012

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background: Acrosome biogenesis is a key event in sperm differentiation that depends on the proper interaction between the Golgi complex and the nuclear envelope of early spermatids. We studied the development, structure and biochemical characteristics of human acrosomes in germ cells and spermatozoa from testicular biopsies and semen samples of fertile men and patients with acrosomeless spermatozoa (globozoospermia). A set of proteins collectively known as the perinuclear theca (PT), which has been related to acrosomal development in many mammalian species, were also investigated. methods:We evaluated spermatozoa from five males with globozoospermia and six fertile men, and immature germ cells from testicular biopsies of one globozoospermic patient and three men with obstructive azoospermia. Samples were assessed by transmission electron microscopy, immunofluorescence microscopy, ultrastructural immunocytochemistry and proteomic analysis by western blot.results: In normal spermiogenesis, the development of the acrosome depends on the correct formation of Golgi-derived proacrosomal vesicles and simultaneous modifications in the nuclear envelope. PT proteins are consistently found in proacrosomic vesicles, localize underneath the acrosome and expand over the nuclear surface along acrosome biogenesis. In fertile men, the PT is composed of six proteins, similar to those previously described for other mammals (16, 22, 29, 34, 50 and 68 kDa). In globozoospermia, abnormal proacrosomal vesicles and paranuclear multivesicular and multilamellar structures were observed that resulted in acrosomes insufficiently developed or detached from the nuclear envelope. PT proteins, dissociated from the acrosomes, were ectopically localized in the cytoplasm. Proteomic analysis showed a significant decrease in all six PT proteins.conclusions: The alterations observed during early acrosome biogenesis in globozoospermia are due to anomalous development of Golgi-derived proacrosomic vesicles, failure of PT proteins to properly associate with the nuclear surface and significant deficiencies in specific PT components that are necessary for proper acrosome formation, implantation and expansion over the spermatid nucleus.

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Section: Discussioncontrasting

confidence: 55%

Acrosomal biogenesis in human globozoospermia: immunocytochemical, ultrastructural and proteomic studies

2012

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“…Round-headed spermatozoa show variable chromatin condensation during morphogenesis. They do not attain the same degree of maturity as do normal ones (Baccetti et al, 1977). The degree of biochemical sperm nuclear maturity can be assessed by aniline blue staining (Terquem & Dadoune, 1983).…”

Section: Introductionmentioning

confidence: 99%

Association between sperm cell chromatin condensation, morphology based on strict criteria, and fertilization, cleavage and pregnancy rates in an IVF program

et al. 2009

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In this study, a total of 95 ejaculates from infertile patients were investigated morphologically according to Kruger's strict criteria and 78 of the 95 ejaculates were stained for chromatin condensation with acidic aniline blue. Patients were divided into two groups based on the percentage of morphologically normal spermatozoa as follows: Men with normal sperm morphology < 14% (Group I), and men with normal morphology > 14% (Group 2). The relationship between percentage of normal sperm morphology and fertilization, cleavage and pregnancy rate was analysed. The rates were 33.7%, 57.1% and 0.0% respectively, in the first group. The corresponding values for the second group were 76.1%) 68.2% and 32.1 YO. The fertilization and pregnancy rates correlate significantly with morphologically normal spermatozoa.In regard to the percentage of morphologically normal spermatozoa stained with aniline blue, patients were divided into two groups: patients with 0-20°/0 stained spermatozoa (Group I) and those with >20% (Group 2). Fertilization and pregnancy rates were higher in the first group than in the second group (79.9%, 52.8% vs. 58.8%, 29.5%).The results demonstrate that chromatin condensation visualized by aniline blue staining is a good predictor for IVF outcome and should be considered besides morphology by sperm assessment for patients undergoing IVF treatment.

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“…This has been demonstrated for round-headed spermatozoa that, due to their normal motility, penetrate the cervical mucus (Jeulin et al -1985), but do not penetrate zona-free hamster eggs (Weissenberg et al -1982). The inabhty to fertilize eggs by round-headed sperm seems due to the lack of the acrosome (Schirren et al -1971;Baccetti et al -1977), and therefore of the acrosomal proteolytic enzymes, like acrosin (Florke-Gerloff et al -1984), that are required for fertilization (Zanefeld 1975). Round-headed sperm represent the whole population of spermatozoa in rare infertile subjects, as a result of a genetic defect (Florke-Gerloff et al ~ 1984), but on the contrary they represent a low percent of ejaculated sperm in fertile donors as well as in a population of infertile patients (Shalgi etal.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence Microscopic Detection of Acrosin in Different Morphologic Types of Human Spermatozoa*/Fluoreszenzmikroskopische Bestimmung von Akrosin bei verschiedenen Formen menschlicher Spermatozoen

et al. 2009

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The immunoreaction for acrosin in different morphological types of human spermatozoa was evaluated by light microscopy in the semen of 68 male partners of infertile couples.The antigen had a cupshaped distribution in the anterior region of the head in normal spermatozoa, and in those with an isolated abnormal mid-piece or tail. On the contrary acrosin was absent, or it did not show a cupshaped immunostaining, in most spermatozoa with malformed heads with the exception of those with a large oval form. The assessment of immunoreaction for acrosin and of vitality of different morphological types of spermatozoa in the same ejaculates, suggested that the antigen was intrinsically absent in abnormal-headed but vital spermatozoa. It is concluded that an inherent lack, or an abnormal synthesis of acrosin during spermatogenesis is associated to the abnormal development of the spermatozoa head.Zusammenfassung: Mittels der Lichtmikroskopie wurde die Immunreaktion f k Akrosin bei verschiedenen Formen menschlicher Spermatozoen im Sperma von 68 Mannern aus' einer infertilen Ehe untersucht. Es zeigte sich eine kappenformige Verteilung des Antigens in der vorderen Region des normalen Spermatozotenkopfes und zwar bei einer isolierten Abnormalitat des Mittelteils oder des Schwanzes. Im Gegensatz dazu fehlte Akrosin bzw. zeigte keine kappenformige Immunfiirbung bei den meisten Spermatozoen mit Fehlbildungen des Kopfes jedoch nicht bei den grokn ovalen Formen. Die Beurteilung der Immunreaktion fir Akrosin und der Vitalitat bei den verschiedenen morphologischen Formen der Spermatozoen in den gleichen Ejakulaten legt es nahe, daB das Antigen wirklich abwesend war bei abnormalen Kopfformen, die jedoch vitalen Spermatozoen angehorten. Hieraus wird die Schluflfolgerung gezogen, daB ein angeborener Mangel oder eine abnormale Synthese des Akrosins wahrend der Spermatogenese mit der abnormalen Entwicklung des Spermatozoenkopfes vergesellschaftet ist.

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Further Observations on the Morphogenesis of the Round Headed Human Spermatozoa

Cited by 51 publications

References 9 publications

Acrosomal biogenesis in human globozoospermia: immunocytochemical, ultrastructural and proteomic studies

Acrosomal biogenesis in human globozoospermia: immunocytochemical, ultrastructural and proteomic studies

Association between sperm cell chromatin condensation, morphology based on strict criteria, and fertilization, cleavage and pregnancy rates in an IVF program

Fluorescence Microscopic Detection of Acrosin in Different Morphologic Types of Human Spermatozoa*/Fluoreszenzmikroskopische Bestimmung von Akrosin bei verschiedenen Formen menschlicher Spermatozoen

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