Further studies of a spectrophotometric method for the determination of diamine oxidase activity

Holmstedt, Bo; Larsson, Lars-Olof; Tham, Richard

doi:10.1016/0006-3002(61)90530-3

Cited by 130 publications

(34 citation statements)

References 9 publications

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“…The oxygen consumption corresponded to the formation of H Z 0 4 . y-Aminobutyraldehyde and ammonia which were assayed by the method of Holmstedt et al 10 ) and the method of Fawcett and Scott,l1) respectively, were not detected in the reaction mixture.…”

Section: Identification Of Reaction Productsmentioning

confidence: 96%

Oxidation of Polyamines by Fungal Enzymes

Yamada

Isobe

Tani

1980

Agricultural and Biological Chemistry

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Section: Identification Of Reaction Productsmentioning

confidence: 96%

Oxidation of Polyamines by Fungal Enzymes

Yamada

Isobe

Tani

1980

Agricultural and Biological Chemistry

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“…DAO (EC 1.4.3.6) and PAO (EC 1.5.3.11) activities were estimated spectrophotometrically using a method based on the colourimetric assay of ∆ 1 -pyrroline using Put (for DAO) or Spd (for PAO) as substrates (Holmstedt et al 1961). Enzyme activity was expressed in pmol Δ 1 -pyrroline/min/g FW using an extinction coefficient of 1.86 × 10 3 mol -1 cm -1 .…”

Section: Methodsmentioning

confidence: 99%

Exogenous application of spermidine enhanced tolerance of pepper against Phytophthora capsici stress

Koç¹

2015

Plant Prot. Sci.

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Koç E. (2015): Exogenous application of spermidine enhanced tolerance of pepper against Phytophthora capsici stress. Plant Protect. Sci., 51: 127-135.The effect of exogenous spermidine -Spd (0.1 and 1 mM) on the relation between polyaminoxidase (PAO), diaminoxidase (DAO), H 2 O 2 , and malondialdehyde (MDA) in three cultivars of pepper (Capsicum annum L.) exhibiting different tolerance to P. capsici stress: KM-Hot (P. capsici-tolerant), , and CM-334 (P. capsici-highly resistant) was investigated. The 0.1 mM Spd pre-treatment led to an increase in DAO activity on the third day in three pepper cultivars under the stress of P. capsici, 1 mM Spd + P. capsici led to an increase in DAO and PAO activities on the fifth day if compared to P. capsici treatment alone. P. capsici alone caused an increase in the amounts of H 2 O 2 at all times in all cultivars and in the amounts of MDA on the third and fifth days in all cultivars. Conversely; under the stress of P. capsici, pre-application of 0.1 mM Spd at all times in KM-Hot and CM-334 cultivars decreased the amount of MDA and H 2 O 2 and on the first and third days in PM-217 cultivar decreased the amount of MDA and H 2 O 2 . This data indicates that exogenous Spd application before inoculation decreases the plasma membrane injury by decreasing the level of H 2 O 2 and regulating the activities of amine oxidase in both P. capsici-sensitive and P. capsici-resistant cultivars of peppers, so it may increase the tolerance of pepper cultivars against P. capsici.

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“…A smapie of the incubation mixture (0.1 ml) was spun down in a flash centrifuge. L11~Pyrroline, a spontaneous cyclization product of y-aminobutyraldehyde, was measured in the supernatant by adding o-aminobenzaldehyde as in a similar method described by Holmstedt et al 4 ) The intensity of the yellow color was estimated by measuring the absorbance at 435 nm. The millimolar extinction coefficient of 1.86 was used for calculations.…”

Section: Methodsmentioning

confidence: 99%