Further studies of myometrial bradykinin receptor-like binding

Fredrick, Mark J.; Vavrek, Raymond J.; Stewart, John M.; Odya, Charles E.

doi:10.1016/0006-2952(84)90212-0

Cited by 29 publications

(7 citation statements)

References 12 publications

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“…Kinin receptor heterogeneity in the guinea-pig ileum has also been proposed by Innis et al (1981), based on correlations between the rank orders of potency of kinin related peptides in competing for Bk-binding sites and in promoting contraction. These authors also provided evidence for the occurrence of a single type of kinin receptor in the rat myometrium, a view that has received increasing support (Odya et al, 1980;Yasujima et al, 1982;Frederick et al, 1984;Frederick & Odya, 1987). Taken together, these studies strongly indicate that kinin receptors can vary between tissues.…”

Section: As Shown Inmentioning

confidence: 82%

The competitive antagonistic effect of compounds from Mandevilla velutina on kinin‐induced contractions of rat uterus and guinea‐pig ileum in vitro

Calixto

Pizzolatti

Yunes

1988

British J Pharmacology

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1 Pure non-peptide compounds obtained in crystal form from the crude extract of the plant Mandevilla velutina (Apocynaceae) were analysed for their antagonistic effects on rat uterine and guineapig smooth muscle contractions induced by bradykinin (Bk), lisyl-bradykinin (L-Bk), acetylcholine (ACh), oxytocin and histamine, in vitro. 3 In the guinea-pig ileum, both MV 8608 and MV 8612 (5 to 20 pgml-1), produced concentration-dependent rightward displacements of the concentration-response curve to Bk (0.1 to 1000 nM) when the experiments were performed in the presence but not in the absence of atropine (2.5 pM). However, in contrast to the result obtained in the rat uterus, compound MV 8608 also caused a significant reduction of the maximal response to Bk. The Schild plot for compound MV 8612 was linear (correlation close to unity) and furnished a nominal pA2 value (g ml 1) of 5.3 and a slope not different from unity.4 In rat uterine muscle, compounds MV 8608 and MV 8612 at concentrations producing marked rightward displacements of the kinin concentration-response curves (10 and 20Ygml 1), did not influence the uterine contractile response to oxytocin or ACh, indicating some selectivity towards kinin receptors. Similarly, compound MV 8612 (10 and 20 ygml 1) did not interfere with the sensitivities or the maximal responses to ACh and histamine in the guinea-pig ileum, whereas compound MV 8608 (10 and 20ug ml-1) caused a slight reduction of ACh-and histamine-induced maximal contractions, allied to decrease of the sensitivity to histamine at concentrations of 20pgml-1 or more.5 These results extend our previous data, indicating the existence of several non-peptide compounds in the crude extract of Mandevilla velutina that act as simple, competitive, selective and reversible kinin receptor antagonists in the rat isolated uterus and guinea-pig ileum smooth muscle.

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Section: As Shown Inmentioning

confidence: 82%

The competitive antagonistic effect of compounds from Mandevilla velutina on kinin‐induced contractions of rat uterus and guinea‐pig ileum in vitro

Calixto

Pizzolatti

Yunes

1988

British J Pharmacology

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“…The data show that there is a time-dependent decrease in BK and Lys-BK during the incubation with the tissue slices though it does not appear to be sufficient to account for the almost 40 fold difference in the potencies of these agonists in the binding and phosphoinositide assays. It is presumed that the radioreceptor assay is measuring intact BK and Lys-BK as there are no hydrolysis products of the peptides that have appreciable affinity for B2 receptors at the concentrations at which they would be present in the assay (Fredrick et al, 1984). Thus, other factors must be responsi-ble for the disparity between kinin agonist activity in the two assays.…”

Section: Resultsmentioning

confidence: 99%

“…Dithiothreitol was included to maintain the sulphhydryl of captopril in a reduced state but this agent has no effect on [3H]-BK binding in our assay. Nevertheless, Manning et al (1986) (Fredrick et al, 1984). Thus, other factors must be responsi-ble for the disparity between kinin agonist activity in the two assays.…”

Section: Radioreceptor Assaymentioning

confidence: 99%

Bradykinin stimulation of phosphoinositide hydrolysis in guinea‐pig ileum longitudinal muscle

Ransom

Goodman

Young

1992

British J Pharmacology

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“…Bovine myometrial membranes were prepared by a procedure originally described by Frederick et al (15) and subsequently modified by Herzig and Leeb-Lundberg (14).…”

Section: ϩmentioning

confidence: 99%

The N Terminus of Bradykinin When Bound to the Human Bradykinin B2 Receptor Is Adjacent to Extracellular Cys20 and Cys277 in the Receptor

Herzig

Nash

Connolly

et al. 1996

Journal of Biological Chemistry

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Further studies of myometrial bradykinin receptor-like binding

Cited by 29 publications

References 12 publications

The competitive antagonistic effect of compounds from Mandevilla velutina on kinin‐induced contractions of rat uterus and guinea‐pig ileum in vitro

The competitive antagonistic effect of compounds from Mandevilla velutina on kinin‐induced contractions of rat uterus and guinea‐pig ileum in vitro

Bradykinin stimulation of phosphoinositide hydrolysis in guinea‐pig ileum longitudinal muscle

The N Terminus of Bradykinin When Bound to the Human Bradykinin B2 Receptor Is Adjacent to Extracellular Cys20 and Cys277 in the Receptor

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