Further studies of the mechanism of counter irritation by turpentine

Abstract

The influence of counter irritation by turpentine (0.2 ml) on zymosan- and carrageenan-oedemas was investigated in the rat. Zymosan-oedema was inhibited by mepyramine and methysergide and by leucopenia. It was not modified by captopril and developed normally in kininogendeficient Brown Norway rats. Leucocytes and mast cell amines but not kinins are thus involved in zymosan-oedema. The last phase of this reaction was inhibited by counter irritation alone, but the odema was largely depressed by counter irritation in rats pretreated with mepyramine and methysergide. Carrageenan-oedema was increased by kininase inhibitors and inhibited by leucopenia in normal rats. This inflammatory reaction had a small developement and was not increased by kininase inhibitors in kininogen-deficient BN rats. Leucocytes and kinins participate in the developement of this inflammatory reaction in normal rats while kinins are lacking in deficient rats. Counter irritation depressed carrageenan-oedema in deficient Brown Norway rats and suppressed the potentiating effect of kininase inhibitors in normal rats. Carrageenan oedema was nearly abolished in turpentine-treated leucopenic rats. These results suggest that the anti-inflammatory effect of counter irritation by turpentine could depend on a reduction of leucocyte accumulation into zymosan-oedema and on a reduction of both kinin formation and of leucocyte accumulation into carrageenan-oedema. The significance of T-kininogen as acute phase reactant is discussed.

Antinociceptive and Anti-Inflammatory Effects of <i>Stachytarpheta jamaicensis </i>(L.) Vahl (Verbenaceae)in Experimental Animal Models

“…Gamache et al [28] have earlier reported that the carrageenaninduced inflammation is a COX-dependent response and is more effectively controlled with inhibitors of arachidonate COX, but not arachidonate lipo-oxygenase inhibitors. This was supported by the finding of Damas et al [29] that carrageenan induced the release of kinins, polymorphonuclear leukocytes as well as proinflammatory factors (e.g. prostaglandins) that contribute to the development of edema.…”

Antinociceptive and Anti-inflammatory Activities of <i>Dicranopteris linearis</i> Leaves Chloroform Extract in Experimental Animals

“…23,30) The carrageenan-induced edema production is associated with the presence of kinins and polymorphonuclear leucocytes, which involved in the release of pro-in‰ammatory mediators like prostaglandins. 31) The ability of the CEDL to reduce the thickness of the edematous hind paw 32,33) indicates the anti-in‰amma-tory properties of the extract. Thisˆnding seems to justify the traditional use of the plant in the treatment of broiler, ulcers or asthma by the people in Papua New Guinea and India.…”

Isolation and properties of a T-kininogenase from bovine erythrocyte membranes

“…The potency of T-kinin to decrease blood pressure was compared to that of standard bradykinin, suggesting the significant ability of T-kininogenase to liberate T-kinin from T-kininogen in vivo (data not shown). These results suggest the hypothesis of Damas et al (1986), who showed that kinin formation could not be induced during inflammation in spite of the elevated level of T-kininogen. Barlas et al (1985) observed an increase in T-kinin in plasma of rats with carrageenin-induced inflammation related to the increase in T-kininogen which does not depend on the activation of a specific Tkininogenase.…”