Further Studies on Comparative Mobility of Labeled Herbicides.

Crafts, A. S.

doi:10.1104/pp.34.6.613

Cited by 33 publications

(12 citation statements)

References 7 publications

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“…It is very difficult to decide whether this influence did really occur. It can only be stated that our results were in agreement with those of Crafts (1959), in whose experiments a dosage of 0-25 \ic (sp. activity 5 mc/ rriM) to the 4th leaf and of 0-05 mc (sp.…”

Section: Discussionsupporting

confidence: 77%

“…1 and 2 clearly show that in barley the changing root/shoot ratio reflects a change in the distribution of organic matter during the development of the plant. This could support the suggestion of Crafts (1959) that the gradual change in photosynthate movement is the cause of changes in 2,4-D distribution. Our results are, however, also in agreement with the opinions of Andersen (1958).…”

Section: Discussionsupporting

confidence: 77%

“…Several studies point out, however, that the movement of 2,4-D from the leaves of cereal plants to other parts of the plants possesses very peculiar characteristics. Crafts (1959) demonstrated that applications of 0-25 pic of 2,4-D in 0-01 ml droplets to barley leaves resulted in intensive movement of the tracer to the root system if treatment (one droplet) was applied to the 1st, 2nd or 3rd leaf. This was not the case, however, after applications of one droplet to the 4th or 5th leaf.…”

Section: Statement Of the Problemmentioning

confidence: 99%

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The Movement of Labelled 2,4‐d in Young Barley Plants

Zweep¹

1961

Weed Research

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Summary. After the 4-or 5-leaf stage in young barley plants, there is a decrease in the translocation of 2,4-D from the leaves to the root system. It is shown that there is no relation between this phenomenon and ear initiation, which occurs at this stage of development. Movement of 2,4-D out of leaves of the tiller in the axils of the 1st and 2nd leaves was also shown to be very small. There is some evidence for a 'block' in the movement of 2,4-D in established grass plants. La migration du 2,4-Z) marqué dans forge

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Section: Discussionsupporting

confidence: 77%

Section: Discussionsupporting

confidence: 77%

Section: Statement Of the Problemmentioning

confidence: 99%

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The Movement of Labelled 2,4‐d in Young Barley Plants

Zweep¹

1961

Weed Research

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“…For example, after 24 hr of incubation in 14C-MH, only 6.5 X 10-'3 mole/mg, fresh weight, is bound in the apical 5 mm, while 2.8 X 10-11 mole/mg is bound in the basal sections. The fact that the amount of bound MH in the root tips does not increase with time between 4 and 12 hr of incubation while the soluble MH increases three-fold might suggest that 2 X 10-11 mole/mg, fresh weight, is a saturation level; however, it is not.…”

Section: Resultsmentioning

confidence: 99%

Metabolism and Binding of ¹⁴C-Maleic Hydrazide

Noodén

1970

Plant Physiol.

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Maleic hydrazide (MH) is taken up by corn and pea seedling roots and bound to some material which is insoluble in 80% ethanol or 5% trichloroacetic acid. 14C-MH is stable metabolically; chromatography of the 80% ethanol-soluble 'IC from treated corn roots and tobacco pith gives no indication of degradation. Very little 14C-MH is bound in the zone of cell division (where MH acts to inhibit root elongation) or even in the region of cell enlargement in corn roots and most is bound 1 or more centimeters behind the tip. Likewise, very little MH is bound in corn coleoptile and tobacco pith sections. About 90% of 14C-MH bound in corn roots is associated with large particles which may be cell wall fragments. The binding is blocked by azide and dinitrophenol, indicating a requirement for metabolic energy; however, inhibitors of protein synthesis (chloramphenicol, puromycin, cycloheximide) and DNA synthesis (fluorodeoxyuridine) do not inhibit binding. Only very small amounts of MH are bound in root homogenates, providing further evidence that the binding process is active. Once the MH is bound in the roots, the complex is stable for at least 1 week. Treatment with 2-aminoethanol releases MH.Over the past 2 decades, maleic hydrazide has attracted a great deal of research effort by plant physiologists and cytologists because of its effects on development and chromosome structure; however, its mechanism of action is still unknown (8,16,24). Several years ago Callaghan and Grun (4) and Baker (2) reported that 14C-MH2 was bound selectively in the nuclei of root tips. Thus it appeared likely that a study on the binding of MH would shed some light on how it acts. This paper is concerned with the uptake of MH, its metabolic stability, and some of the characteristics of its binding to some unidentified macromolecule(s). A preliminary report of these findings has been made (14). MATERIALS AND METHODSSeeds of Zea mays Michigan 250 RF hybrid (a yellow field corn) were surface sterilized and germinated in vermiculite as described elsewhere (15). The corn coleoptile sections, tobacco pith explants, and pea seedlings (Pisum sativum variety Alaska)were also grown and prepared as before (15,16 14C-Maleic hydrazide with the label in the 1-position and a specific radioactivity of 4.12 mc/mmole was obtained from Tracerlab, Waltham, Mass. The radiochemical purity of all batches of '4C-MH was checked by descending chromatography using three solvent systems. These were n-butanol, concentrated acetic acid, H20 (6:1:2, v/v); i-propanol, concentrated ammonia, H20 (60:15:25, v/v); and 72% crystalline reagent phenol, 28% H20 (w/v). More than 99% of the 14C-MH was in the MH peak; no other peaks of radioactivity could be detected. In the three solvents used above the RFS of "4C-MH were 0.7 to 0.8.To determine the rates of uptake and binding of 'IC in corn roots, the seedlings were incubated with their roots in aerated 14C-MH solution containing chloramphenicol to suppress bacterial growth (15,18,22). After thorough washing and excision, the ro...

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“…righllt) has mllovedl througlhout the hvpocotvl nid roots in high concentratioins in both the 3-lhourl-(4a ) and 6-holutr ( 41) ) treatmlent timies. In the treated cotvledonls the ty)pical wedge-shaped paltterlns of apoplastic movement (8) (6 ). Sucrose.…”

Section: Resultsmentioning

confidence: 99%