Fusaramin, an antimitochondrial compound produced by Fusarium sp., discovered using multidrug-sensitive Saccharomyces cerevisiae

Traminines A and B, produced by Fusarium concentricum, inhibit oxidative phosphorylation in Saccharomyces cerevisiae mitochondria

“…Fungal strain FKI-7550 was isolated from soil around the root of Cerasus × yedoensis collected in Tokushima, Japan. We have already reported that the fusaramin producing strain FKI-7550 was assigned to the genus and was designated as Fusarium concentricum (NITE-2944 [Sakai et al., 2019 ]). One loopful of strain Fusarium concentricum FKI-7550 grown on an LcA slant (0.1% glycerol, 0.08% KH 2 PO 4 , 0.02% K 2 HPO 4 , 0.02% MgSO 4 ·7H 2 O, 0.02% KCl, 0.2% NaNO 3 , and 1.5% agar, pH 6.0) was inoculated into a 500 ml-Erlenmeyer flask containing 100 ml of a pre-seed culture medium [2% glucose, 0.2% yeast extract, 0.05% MgSO 4 ·7H 2 O, 0.5% Polypeptone (FUJIFILM Wako Pure Chemical Co., Osaka, Japan), 0.1% KH 2 PO 4 , and 0.1% agar, pH 6.0] and incubated on a rotary shaker at 27°C for 4 days.…”

“…We selected microbial culture broths, in which the growth of the S. cerevisiae 12geneΔ0HSR-iERG6 was inhibited in glycerol-containing medium (YPG agar medium; 1% yeast extract, 2% peptone, 3% glycerol, and 1.5% agar) but not in glucose-containing medium (YPD agar medium; 1% yeast extract, 2% peptone, 3% glucose, and 1.5% agar). This approach using two different carbon sources has been used to isolate compounds targeting mitochondrial oxidative phosphorylation (Sakai et al., 2019 ; Suga et al., 2015 ; Watanabe et al., 2017 ). Based on the above strategies, we revealed that Fusarium concentricum FKI-7550 produced two new tetramic acid derivatives; namely, traminines A ( 1 ) and B ( 2 ).…”

Comprehensive Overview of Progress in Functionalization of 2‐Pyridone and 2, 4 ‐Dihydroxy Pyridine: Key Constituents of Vital Natural Products

“…Sambutoxin (15) and N ‐demethyl sambutoxin (16) (Figure 5) are pyridone alkaloids isolated from culture broth of Fusarium sp. FKI‐7550 [22] . These compounds inhibit mitochondrial electron transport chain and are used in human cancer treatment exhibiting cytotoxic action against various cancer cell lines.…”

“…FKI-7550. [22] These compounds inhibit mitochon- ChemistrySelect drial electron transport chain and are used in human cancer treatment exhibiting cytotoxic action against various cancer cell lines. In the same way, citridone B, (17) (Figure 5) obtained from Penicillium sp.…”

“…N-substituted 2-pyridones are important chemical compounds on account of their chemical reactivity, photochemical behaviour, [124,125,126] biological activities, [127,128,129,131] and are present frequently in natural products such as ricinine (5), sambutoxin (15), leporin A (27) etc. having potent insecticidal, [14] antitumor [22] and antibacterial activity, [30] respectively. N-alkyl pyridone is used as a model compound for cytisine [131] (4), a natural product (Figure 1…”