Fusarium circinatum isolates from northern Spain are commonly infected by three distinct mitoviruses

Vainio, Eeva J.; Martínez‐Álvarez, Pablo; Bezos, Diana; Hantula, Jarkko

doi:10.1007/s00705-015-2462-7

Cited by 17 publications

(30 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two paired monosporic cultures (mycovirus- infected and mycovirus-free) were used per isolate (Table 1). The presence of different mycovirus strains in each isolate was reported in a previous study by our research group (Vainio et al, 2015). In the present study, the presence or absence of mycoviruses (FcMV1 and/ or FcMV2-2) was confirmed according to Martínez-Álvarez et al (2014b).…”

Section: Fungal Isolatessupporting

confidence: 82%

“…The effects of mycoviruses on spore production and pathogenesis have also been widely reported (Kazmierczak et al, 1996;Wang et al, 2014, Zamora et al, 2016, indicating the potentially damaging effects of the viruses on plant health. Three different species of the genus Mitovirus that infect F. circinatum have recently been identified as putative members of Narnaviridae (genus Mitovirus) and designated Fusarium circinatum mitovirus 1, 2-1 and 2-2 (FcMV1, FcMV2-1 and FcMV2-2) (Martínez-Álvarez et al, 2014a;Vainio et al, 2015;Muñoz-Adalia et al, 2016a).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Short communication: Effect of mycoviruses on growth, spore germination and pathogenicity of the fungus Fusarium circinatum

et al. 2018

Self Cite

View full text Add to dashboard Cite

Aim of the study: To assess the impact on two mycoviruses recently described in F. circinatum mitovirus 1 and 2-2 (FcMV1 and FcMV2-2) on i) mycelial growth, ii) spore germination and iii) relative necrosis.Material and methods: Fourteen monosporic strains of F. circinatum (one of each pair infected with mycoviruses and the other without them) of the pathogen with and without viruses were selected for the assay. The statistical analysis, were a linear mixed model of analysis of variance considering one between-subjects factor (isolate) and one within-subjects factor with four levels (1=without viruses, 2=only virus FcMV1, 3=only virus FcMV2-2 and 4=both viruses).Main results: Colony growth rates of F. circinatum isolates were significantly reduced in presence of mycoviruses (p=0.002). The spore germination was also reduced in the F. circinatum isolates containing mycovirus as compared to mycovirus-free isolates (p<0.001). No significant differences in lesion lengths caused by F. circinatum were found in relation to the presence/absence of mycovirus (p<0.61).Research highlights: Reduction of the percentage of spore germination in the isolates of F. circinatum with mycovirus infections, as compared to free isolates, provides indications of reduction of metabolic activity and plant physiology are discussed. The lack of significant differences found in the length of the lesions caused by F. circinatum with respect to the presence/absence of mycovirus, indicates that further studies with a larger number of variables are required Additional keywords: pine pitch canker; hypovirulence; biological control; forest pathology.

show abstract

Section: Fungal Isolatessupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Short communication: Effect of mycoviruses on growth, spore germination and pathogenicity of the fungus Fusarium circinatum

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…The cDNA was used as a template for PCR with the following virus-specific primers: FMC1F1 (5 -CGTGGATTAAAACCCACAAA-3 ), FMC1Rev1 (5 -TGGTAATCTACCATAGCAATTAYTC-3 ), FMC3F1 (5 -GAYAGAACTTTTACTCAAGATCC-3 ) and FMC3Rev1 (5 -ATTCATCTYTTGGCAAATTCATA-3 ) [16]. The primer pair FMC1F1/FMC1Rev1 was specific to FcMV1, whereas FMC3F1/FMC3Rev1 was used to detect FcMV2-2.…”

Section: Total Rna Extraction and Pcrmentioning

confidence: 99%

“…These mitoviruses are common in F. circinatum isolates from northern Spain. They have been shown to be polymorphic [16] and their viral genome has recently been studied [17]. The main aim of the present study was to investigate how the probability and rate of transmission of these mitoviruses via microconidia vary in relation to the fungal isolate and strain of virus (FcMV1 and FcMV2-2).…”

Section: Introductionmentioning

confidence: 99%

Vertical Transmission of Fusarium circinatum Mitoviruses FcMV1 and FcMV2-2 via Microconidia

Romeralo

Bezos

Martínez‐Álvarez

2018

Forests

Self Cite

View full text Add to dashboard Cite

Pine Pitch Canker disease, caused by the pathogenic fungus Fusarium circinatum, affects conifer species worldwide. However, the virulence of the pathogen may be affected by the presence of mycoviruses. The aim of this laboratory-based study was to investigate the probability and rate of transmission of F. circinatum mitoviruses FcMV1 and FcMV2-2 via microconidia. Ten isolates of mitovirus-infected F. circinatum were subcultured to produce a total of 100 single-spore colonies (ten replicates per isolate). The total RNA and cDNA obtained from each spore isolate (monosporic culture) were amplified by PCR with specific primers for detection of F. circinatum mitoviruses FcMV1 and FcMV2-2. The mitoviruses were detected in a high percentage of the individual spore isolates (between 60% and 100% depending on the fungal isolate). However, the probability of transmission was not statistically significantly associated with either the F. circinatum isolate or the viral strain. A high proportion of transmission via microconidia is critical for development of a biological control program against Pine Pitch Canker (PPC) disease in forests. However, further studies are needed to establish the effect of these mitoviruses on the virulence of F. circinatum.

show abstract

“…Fusarium circinatum hosts several species and types of mitoviruses (Martínez‐Álvarez et al ., ; Vainio et al ., ), but the role the viruses play in pathogen virulence is not clear. While virulence of F. circinatum was enhanced on 1‐year‐old P. radiata seedlings (Muñoz‐Adalia et al ., ), mycelial growth of F. circinatum colonies and spore germination were significantly reduced by the virus presence under in vitro conditions (Flores‐Pacheco et al ., ).…”

Section: The Use Of Biological Control For Combating F Circinatummentioning

confidence: 99%

Environmentally friendly methods for controlling pine pitch canker

et al. 2019

View full text Add to dashboard Cite

Fusarium circinatum is a highly virulent invasive pathogen that causes the disease commonly known as pine pitch canker (PPC). On mature trees, the most common symptoms of PPC include resin bleeding, resinous cankers, wilting of needles and dieback. In nurseries the main symptoms are yellowish needles, and wilting of foliage and shoots. PPC is considered one of the most important diseases of conifers globally and at least 60 species of Pinus along with Pseudotsuga menziesii are known to be susceptible. Quarantine regulations are crucial to minimize the risk of new introductions into disease‐free countries. However, if these measures fail, implementation of environmentally friendly control methods is currently encouraged in an integrated management approach that minimizes the use of chemicals in forests. This review therefore summarizes current knowledge of biological control using endophytic fungi, bacteria and viruses, and other environmentally friendly methods to control PPC, including thermotherapy, natural products with fungicidal effects, promoters of innate host resistance mechanisms and breeding for resistance. Key research gaps to be addressed for improvement of effective management of this disease are discussed.

show abstract

Fusarium circinatum isolates from northern Spain are commonly infected by three distinct mitoviruses

Cited by 17 publications

References 22 publications

Short communication: Effect of mycoviruses on growth, spore germination and pathogenicity of the fungus Fusarium circinatum

Short communication: Effect of mycoviruses on growth, spore germination and pathogenicity of the fungus Fusarium circinatum

Vertical Transmission of Fusarium circinatum Mitoviruses FcMV1 and FcMV2-2 via Microconidia

Environmentally friendly methods for controlling pine pitch canker

Contact Info

Product

Resources

About