Fusarium mangiferae as New Cell Factories for Producing Silver Nanoparticles

Abstract

Finding a safe and broad-spectrum medication is a goal of scientists, pharmacists, and physicians, but developing and fabricating the right medicine can be challenging. The current study describes the formation of silver nanoparticles (AgNPs) by Fusarium mangiferae. It involves the antibiofilm activity of the nanoparticles against Staphylococcus aureus. It also involves cytotoxic effect against mammalian cell lines. Well-dispersed nanoparticles are formed by F. mangiferae. The sizes of the nanoparticles were found to range from 25 to 52 nm, and UV-Vis scan showed absorption around 416-420 nm. SEM, TEM, and AFM results displayed spherical and oval shapes. Furthermore, the FTIR histogram detected amide I and amide II compounds responsible for the stability of AgNPs in an aqueous solution. AgNPs were observed to decrease the formation of biofilm at 75% (v/v). DNA reducing, smearing, and perhaps fragmentation were noticed after treating the bacterial cells with 50% (v/v). Additionally, cell lysis was detected releasing proteins in the supernatant. It was also observed that the AgNPs have the ability to cause 59% cervical cancer cell line (HeLa) deaths at 25% (v/v), however, they showed about 31% toxicity against rat embryo fibroblast transformed cell lines (REF). The results of this study prove the efficiency of AgNPs as an antibiofilm against S. aureus, suggesting that AgNPs could be an alternative to antibiotics. It must also be emphasized that AgNPs displayed cytotoxic behavior against mammalian cell lines. Further studies are needed for assessing risk in relation to the possible benefit of prescribing AgNPs.

In vitro biofabrication of silver nanoparticles: Optimization, characterization, and activity against beta-lactamases-resistant Enterococcus faecalis

“…The bio lm degrading activity of AgNPs was determined by colorimetric method against E. faecalis according to (Hamzah et al 2018) with a few modi cations. A culture of E. faecalis was grown in LB broth for 24 h at 37°C.…”

“…Congo red dye (Sigma-Aldrich, Germany) (0.8 g/L) was also prepared simultaneously and added to warm (50°C) BHI agar. The color and texture of the colonies were then analyzed to evaluate bio lm e cacy (Hamzah et al 2018). This experiment was conducted in triplets with averages and standard deviations (STDEV) calculated using Microsoft Excel.…”

Phenotypic and Genetic Effects of Wi-Fi Waves on Some Bacterial Species Isolated from Otitis Media Infection

“…Genomic DNA was extracted from bacterial isolates before and after exposure to Wi-Fi radio waves as described by Onasanya, 25 and Hamzah et al, 26 with slight modifications. One milliliter of the bacterial culture was transferred to 1.5 mL Eppendorf tube and centrifuged at 14,000 rpm for 3 min.…”

Biosynthesis and characterization of silver nanoparticles using Cinnamomum zeylanicum extract anda study of antibacterial effect against multi-drug resistance Gram-negativebacteria

“…One mL of cinnamon bark extract was added to an amber glass vial containing 50 mL of a 1 mM aqueous silver nitrate solution (AgNO3). The mixture was incubated at room temperature with continuous shaking stirring for 18 hours to produce silver nanoparticles (16).…”

“…The important characteristics of Ag-NPS have been identified to ensure the formation of silver nanoparticles, including the presence of a surface specific plasmon resonance peak, the size and shape of the particles, and the effective groups arranged by particles using the following assays (13,16).…”