Fusarium oxysporum f. sp. lycopersici races and their genetic discrimination by molecular markers in West Mediterranean region of Turkey

Baysal, Ömür; Siragusa, Maria Gabriella; Ikten, Hatice; Polat, İlknur; Gümrükcü, Emine; Yiğit, Fahri; Carimi, Francesco; Silva, J.A. Teixeira da

doi:10.1016/j.pmpp.2009.09.008

Cited by 38 publications

(38 citation statements)

References 33 publications

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“…Also, the similarity index for Jaccard’s similarity coefficient was 0.71, which is in agreement with the findings by Bayraktar (). The genetic diversity among the Indian F. oxysporum strains revealed by ISSR analysis was in concordance with many earlier findings including our present results in F. oxysporum from different crops (Baysal et al., ). Baysal et al.…”

Section: Discussionsupporting

confidence: 93%

Genetic and virulence variation in Fusarium oxysporum f. sp. cepae causing root and basal rot of common onion in Iran

Nasr-Esfahani

2018

Journal of Phytopathology

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Root and basal rot of common onion (Allium cepae L.) caused by Fusarium oxysporum f. sp. cepae is one of the most important diseases causing tremendous losses in onion‐growing areas worldwide. In this study, random amplified polymorphic DNA (RAPD), intersimple sequence repeats (ISSR) and virulence studies were conducted to analyse 26 F. oxysporum f. sp. cepae isolates obtained from the main onion‐growing regions of Iran, including Fars, Azerbaijan and Isfahan states. Cluster analysis using UPGMA method for both RAPD and ISSR markers revealed no clear grouping of the isolates obtained from different geographical regions, and the isolates were observed to derive probably from the same clonal lineage. Pathogenicity test indicated that all F. oxysporum f. sp. cepae isolates were pathogenic on onion; however, virulence variability was observed among the isolates. The grouping based on virulence variability was not correlated with the results of RAPD and ISSR analyses.

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Section: Discussionsupporting

confidence: 93%

Genetic and virulence variation in Fusarium oxysporum f. sp. cepae causing root and basal rot of common onion in Iran

Nasr-Esfahani

2018

Journal of Phytopathology

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“…To understand the evolutionary history and genomic constituents of the formae speciales within F. oxysporum requires knowledge of the phylogenetic relationships among isolates (Appel & Gordon, 1996). Over the past several years, genetic diversity in F. oxysporum has been examined using various genetic markers, such as isozyme profiles (Bosland & Williams, 1987), restriction fragment length polymorphisms (RFLP) in mitochondria and nuclear DNA (Jacobson & Gordon, 1990) and inter-simple sequence repeat (ISSR), (Baysal et al, 2009). Phylogenetic analyses based on DNA sequences of housekeeping genes such as the mitochondrial small subunit (mtSSU), ribosomal RNA gene, rDNA intergenic spacer (IGS) region, and translation elongation factor (TEF)-1a gene were extensively studied for genetic and evolutionary relationships within and among the formae speciales of F. oxysporum (O'Donnell et al, 1998;Lievens et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

A comparative in silico analysis on frequency and distribution of microsatellites in coding regions of three formae speciales of Fusarium oxysporum and development of EST-SSR markers for polymorphism studies

Mahfooz

Maurya

Srivastava

et al. 2012

FEMS Microbiol Lett

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Fusarium oxysporum is a ubiquitous species complex of soil-borne plant pathogens comprising of many different formae speciales, each characterized by a high degree of host specificity. In the present investigation, we surveyed microsatellites in the available express sequence tags and transcript sequences of three formae speciales of F. oxysporum viz. melonis (Fom), cucumerium (Foc), and lycopersici (Fol). The relative abundance and density of microsatellites were higher in Fom when compared with Foc and Fol. Thirty microsatellite primers were designed, ten from each forma specialis, for genetic characterization of F. oxysporum isolates belonging to five formae speciales. Of the 30 primers, only 14 showed amplification. A total of 28 alleles were amplified by 14 primers with an average of two alleles per marker. Eight markers showed 100% polymorphism. The markers were found to be more polymorphic (47%) in Fol as compared to Fom and Foc; however, polymorphic information content was the maximum (0.899) in FocSSR-3. Nine polymorphic markers obtained in this study clearly demonstrate the utility of newly developed markers in establishing genetic relationships among different isolates of F. oxysporum.

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“…The core sequence is followed by three selective nucleotides at the 3 0 -end of each primer (Li and Quiros 2001). In recent years, ISSR and SRAP have been recognized as useful molecular systems for studying fingerprint and genetic diversity in fungi because of their simplicity, reproducibility, and polymorphism (Grunig et al 2001;Wang et al 2005;Gryta et al 2006;Anh Nghia et al 2008;Yu et al 2008;Baysal et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis of Monascus strains using SRAP and ISSR markers

Shao

Chen

2011

Mycoscience

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In this study, sequence-related amplification polymorphism (SRAP) and inter-simple sequence repeat (ISSR) were analyzed for accessing the genetic diversity of 37 Monascus isolates and 14 control strains. According to the dendrogram produced by SRAP data, all the tested strains were grouped into four clusters at a 78% similarity level. Comparatively, 51 tested strains were divided into four major groups at a similarity level of 74% based on the dendrogram generated via ISSR marker analysis. Based on the two sets of dendrograms, Monascus aurantiacus, M. purpureus, M. serorubescens, M. anka, and M. ruber were clustered in the same clade; M. albidus, M. fuliginosus, and M. barkeri were clustered with M. pilosus in a second clade; and M. lunisporas and M. argentinensis occurred together in a third cluster distinct from the other Monascus species. The cluster result produced by SRAP data shared great similarity with that by ISSR data with minor differences in the subgroups, which is basically in agreement with morphological observations. In general, SRAP and ISSR are more simple, rapid, and efficient, which may provide alternative molecular approaches to studying genetic diversity, classification, and identification of Monascus strains.

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Fusarium oxysporum f. sp. lycopersici races and their genetic discrimination by molecular markers in West Mediterranean region of Turkey

Cited by 38 publications

References 33 publications

Genetic and virulence variation in Fusarium oxysporum f. sp. cepae causing root and basal rot of common onion in Iran

Genetic and virulence variation in Fusarium oxysporum f. sp. cepae causing root and basal rot of common onion in Iran

A comparative in silico analysis on frequency and distribution of microsatellites in coding regions of three formae speciales of Fusarium oxysporum and development of EST-SSR markers for polymorphism studies

Genetic diversity analysis of Monascus strains using SRAP and ISSR markers

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