Fusarium solani and F. oxysporum: Etiological agents of damping off in crambe

Migliorini, Patrícia; Milanesi, Paola Mendes; Mezzomo, Ricardo; Maciel, Caciara Gonzatto; Muniz, Marlove Fátima Brião

doi:10.5039/agraria.v13i1a5510

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…As a matter of consequence, the identification based on the sequencing of ITS region and others regions, such as EF-1α, has provided an alternative approach for higher detection and identification of Fusarium spp. [41][42][43].…”

Section: Discussionmentioning

confidence: 99%

First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

et al. 2019

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The aim of this study was to compare the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), phenotypic and molecular methods for the identification of Fusarium species complexes isolated from clinical cases in the State of Sao Paulo (Brazil) between the years 2001 and 2017. Sequencing of ITS region of ribosomal DNA and elongation factor 1 alpha gene (ET1α) were used as reference method in the analysis of a total of 108 Fusarium spp. clinical strains isolated from human hosts with superficial and systemic infections. Agreement between MALDI-TOF-MS and molecular data was observed for 97 out of 108 clinical isolates (89.8%), whereas five (4.6%) and six (5.5%) clinical isolates were misidentified and were not identified by MALDI-TOF MS, respectively. ITS region sequences and MALDI-TOF MS mass spectra identified and grouped correctly most of Fusarium clinical isolates at species complex level. This investigation highlights the potential of MALDI-TOF MS technique as a fast and cost-efficient alternative for clinical Fusarium identification. However, MALDI-TOF MS requires a more accurate and larger database. This work is the first comprehensive report for Fusarium population, based on phenotypic analyses, proteomic profile by MALDI-TOF and phylogenetic analyses of Fusarium species complexes isolated from clinical cases in the State of Sao Paulo, Brazil.

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Section: Discussionmentioning

confidence: 99%

First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

et al. 2019

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“…Among all the pathogens that can infect soybean, Phytophthora sojae has been considered as one of the main causes of soybean damping‐off in southern Brazil (Costamilan et al, 2013) along with Rhizoctonia solani (Fenille et al, 2002) and Fusarium spp. (Migliorini et al, 2018). However, it has been reported that oomycetes belonging to the Pythium complex can also cause soybean damping‐off (Broders et al, 2007; Dorrance et al, 2004; Radmer et al, 2017; Rojas et al, 2017; Zitnick‐Anderson & Nelson, 2015).…”

Section: Introductionmentioning

confidence: 99%

Damping‐off of soybean in southern Brazil can be associated with different species of Globisporangium spp. and Pythium spp.

Molin

Ribeiro²,

Matsumoto³

et al. 2021

Plant Pathology

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Damping‐off of soybean is a common problem in some fields in southern Brazil. Frequently, Phytophthora sojae has been pointed out as the main causal agent. However, questions were raised as to whether other oomycetes could also be present. Hence, the objectives of this study were to isolate, identify, and determine the pathogenicity and aggressiveness of oomycetes isolated from soybean seedlings. Thirty‐three isolates were obtained from 13 fields. Isolates were identified based on morphology and molecular methods. Nine species were recovered: Pythium conidiophorum (n = 14, 42.4%), Globisporangium rostratifingens (n = 7, 21.2%), G. irregulare (n = 2, 6.1%), G. spinosum (n = 1, 3.0%), G. ultimum var. sporangiiferum (n = 2, 6.1%), Pythium acanthicum (n = 2, 6.1%), P. deliense (n = 1, 3.0%), P. inflatum (n = 1, 3.0%), and P. torulosum (n = 3, 9.1%). Pathogenicity and aggressiveness were determined with two soybean cultivars (DM57i52 and NEO530) in a seed rot assay and a root rot assay. All isolates were pathogenic to soybean with variable levels of aggressiveness in both assays. The range of seed disease severity index varied from 7.5 to 100 for DM57i52 and from 23.1 to 100 for NEO530. The mean root disease severity index of the soybean cultivars ranged from 29.4 to 92.8. This is the first official report of G. irregulare, G. rostratifingens, G. spinosum, G. ultimum var. sporangiiferum, P. acanthicum, P. conidiophorum, P. deliense, P. inflatum, and P. torulosum causing damping‐off of soybean in the state of Rio Grande do Sul in southern Brazil.

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“…In addition, other pathogens such as Rhizoctonia solani (Fenille et al, 2002), Fusarium spp. (Migliorini et al, 2018) and Globisporangium/Pythium complex (Molin et al, 2021a) have also been associated with the disease. There is some information on the reaction of Brazilian soybean cultivars to R. solani (Ben, 2015) and Fusarium (Gásperi et al, 2003); however, little is known about their reaction to Globisporangium/Pythium species.…”

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confidence: 99%

Soybean cultivars adapted to southern Brazil differ in their reaction to Globisporangium irregulare, G. ultimum var. sporangiiferum and Pythium conidiophorum

Molin

Ribeiro²,

Matsumoto³

et al. 2022

Plant Pathology

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Brazil is the largest soybean producer in the world (USDA, 2022). The area cultivated with soybean in the country is divided into macroregions due to the differences in environmental conditions (Kaster & Farias, 2012), which require cultivars adapted to each macroregion.Damping-off of soybean is a common problem reported by several growers in Macroregion 1 (Costamilan et al., 2013;Molin et al., 2021a), which includes the southern region of Brazil (Kaster & Farias, 2012). Symptoms of the disease include seed decay and root rot (Broders et al., 2007;Rojas et al., 2017), which can compromise seed germination, seedling emergence and initial establishment of

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Fusarium solani and F. oxysporum: Etiological agents of damping off in crambe

Cited by 3 publications

References 17 publications

First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

Damping‐off of soybean in southern Brazil can be associated with different species of Globisporangium spp. and Pythium spp.

Soybean cultivars adapted to southern Brazil differ in their reaction to Globisporangium irregulare, G. ultimum var. sporangiiferum and Pythium conidiophorum

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