Fusarium species recovered from the water distribution system of a French university hospital

Sautour, Marc; Edel-Hermann, Véronique; Steinberg, Christian; Sixt, Nathalie; Laurent, Julie; Dalle, Frédéric; Aho, Serge; Hartemann, Philippe; l'Ollivier, Coralie; Goyer, Marianne; Bonnin, Alain

doi:10.1016/j.ijheh.2011.11.003

Cited by 41 publications

(33 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fusaria in water may be aerosolized into the air when the water passes through taps and showers and then inhaled by immunocompromised patients (14). In two previous studies, we detected F. oxysporum in the water distribution systems of different French hospitals, with high concentrations of up to 10 5 propagules liter Ϫ1 in some units (19,20). Heterogeneous distributions according to building and variability over time were explained by episodic detachments of parts of colonies from biofilms in the pipes.…”

mentioning

confidence: 70%

“…This study included 68 isolates of F. oxysporum that were collected from the water distribution systems of different hospitals located in two cities in eastern France, Dijon and Nancy, which lie 230 km apart. Forty-six isolates were collected from February to September 2009 in hospitals D1 and D2 located in Dijon (19), and 22 isolates were collected from October 2009 to October 2010 in hospital D3 located in Dijon and hospitals N1 and N2 located in Nancy (20) ( Table 1). The 68 isolates were selected from 232 isolates that were previously characterized to be representative of all units sampled in the different hospitals and at all sampling times (see Table S1 in the supplemental material).…”

Section: Methodsmentioning

confidence: 99%

“…With the exception of three previously analyzed isolates, all of the isolates displayed identical TEF1 sequences (19,20). The isolates were further characterized using microsatellite markers that were developed by Cheng-Hua Huang for populations of F. oxysporum f. sp.…”

Section: Methodsmentioning

confidence: 99%

“…In a previous study, Edel-Hermann et al (29) analyzed Fusarium diversity in soils sampled at three different locations (E, B, and M) in eastern France near Dijon. The TEF1 sequences of 100 F. oxysporum isolates from each of the three soils were compared with the TEF1 sequences of isolates from water that were previously described (19,20). Sequences were aligned and compared with the Kimura two-parameter distance model and the neighbor-joining (NJ) method using SeaView (30).…”

Section: Methodsmentioning

confidence: 99%

“…Analysis of partial sequences of translation elongation factor 1␣ (TEF1) showed low genetic diversity within these strains. Most of the isolates collected from water displayed the same sequence, even those from hospitals in two distant cities, suggesting the existence of a widespread clonal lineage in the water networks of hospitals (19,20). The question then arose as to the origin of this lineage.…”

mentioning

confidence: 99%

See 4 more Smart Citations

A Clonal Lineage of Fusarium oxysporum Circulates in the Tap Water of Different French Hospitals

Edel-Hermann

Sautour

Gautheron

et al. 2016

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Fusarium oxysporum is typically a soilborne fungus but can also be found in aquatic environments. In hospitals, water distribution systems may be reservoirs for the fungi responsible for nosocomial infections. F. oxysporum was previously detected in the water distribution systems of five French hospitals. Sixty-eight isolates from water representative of all hospital units that were previously sampled and characterized by translation elongation factor 1␣ sequence typing were subjected to microsatellite analysis and full-length ribosomal intergenic spacer (IGS) sequence typing. All but three isolates shared common microsatellite loci and a common two-locus sequence type (ST). This ST has an international geographical distribution in both the water networks of hospitals and among clinical isolates. The ST dominant in water was not detected among 300 isolates of F. oxysporum that originated from surrounding soils. Further characterization of 15 isolates by vegetative compatibility testing allowed us to conclude that a clonal lineage of F. oxysporum circulates in the tap water of the different hospitals. IMPORTANCEWe demonstrated that a clonal lineage of Fusarium oxysporum inhabits the water distribution systems of several French hospitals. This clonal lineage, which appears to be particularly adapted to water networks, represents a potential risk for human infection and raises questions about its worldwide distribution. N osocomial infections caused by members of the genus Fusarium have emerged over the past 2 decades (1, 2). Infections caused by Fusarium isolates may be superficial, such as keratitis and onychomycosis, but Fusarium spp. can also cause life-threatening disseminated infections in immunocompromised patients (3). The Fusarium species most often involved are the morphological species Fusarium solani and Fusarium oxysporum (4, 5). These morphological species are now recognized as species complexes due to their high level of phylogenetic diversity (6, 7).Members of the genus Fusarium have a diverse ecology that is linked to an impressive metabolic diversity together with a high genetic diversity. They are primarily known as saprophytes in the environment and are usually found in all types of soils (8-10), and they are frequently associated with plants as saprophytes, endophytes, or pathogens (9, 11). Fusaria may also be found in aquatic habitats, including natural water environments (12, 13) and drinking water distribution systems (14-17).Water distribution systems in hospitals were identified as potential reservoirs for species of Fusarium, where they are thought to be responsible for nosocomial infections (14, 18). Fusaria in water may be aerosolized into the air when the water passes through taps and showers and then inhaled by immunocompromised patients (14). In two previous studies, we detected F. oxysporum in the water distribution systems of different French hospitals, with high concentrations of up to 10 5 propagules liter Ϫ1 in some units (19,20). Heterogeneous distributions according to...

show abstract

mentioning

confidence: 70%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%