Fusion and endocytosis of anionic liposomes with Ehrlich ascitic carcinoma cells

Goryacheva, Yu. A.; Vekshina, O. M.; Yashin, V. A.; Kim, Yu. A.

doi:10.1007/s10517-006-0069-4

Cited by 10 publications

(5 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Only few authors, reporting on highly efficient multi-component lipoplexes, have previously hypothesized that lipid mixing entropy may play a key role in the mechanism of internalization [40]. Several lipids (DOTAP, DOTMA, DOPE, CL) and polymers (PEG) that may facilitate lipoplex fusion with the plasma membrane [1], [3], [41]–[43] or with the endosome membrane [44] have been described. Nucleic acid delivery is efficiently achieved by viruses which penetrate through the plasma membrane by spontaneous merger of membranes promoted by fusion protein catalysts.…”

Section: Discussionmentioning

confidence: 99%

“…Non-leaky fusion of the vesicles over rapid collapse into H II structures was observed at temperatures ranging between 0 and 50°C [34], [45]. Membrane fusion of a synthetic vesicle in live cells is poorly described [41]–[43]. Biological membrane fusion is a localized, fast (less than a millisecond) and a well controlled (non leaky) process.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Physico-Chemical Characteristics of Lipoplexes Influence Cell Uptake Mechanisms and Transfection Efficacy

Resina

Prévôt²,

Thierry³

2009

PLoS ONE

View full text Add to dashboard Cite

BackgroundFormulation of DNA/cationic lipid complexes (lipoplexes) designed for nucleic acid delivery mostly results in positively charged particles which are thought to enter cells by endocytosis. We recently developed a lipoplex formulation called Neutraplex that allows preparation of both cationic and anionic stable complexes with similar lipid content and ultrastructure.Methodology/Principal FindingsTo assess whether the global net charge could influence cell uptake and activity of the transported oligonucleotides (ON), we prepared lipoplexes with positive and negative charges and compared: (i) their physicochemical properties by zeta potential analysis and dynamic light scattering, (ii) their cell uptake by fluorescence microscopy and flow cytometry, and (iii) the biological activity of the transported ON using a splicing correction assay. We show that positively or negatively charged lipoplexes enter cells cells using both temperature-dependent and -independent uptake mechanisms. Specifically, positively charged lipoplexes predominantly use a temperature-dependent transport when cells are incubated OptiMEM medium. Anionic lipoplexes favour an energy-independent transport and show higher ON activity than cationic lipoplexes in presence of serum. However, lipoplexes with high positive global net charge and OptiMEM medium give the highest uptake and ON activity levels.ConclusionsThese findings suggest that, in addition to endocytosis, lipoplexes may enter cell via a temperature-independent mechanism, which could be mediated by lipid mixing. Such characteristics might arise from the specific lipoplex ultrastructure and should be taken into consideration when developing lipoplexes designed for in vivo or ex vivo nucleic acid transfer.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Physico-Chemical Characteristics of Lipoplexes Influence Cell Uptake Mechanisms and Transfection Efficacy

Resina

Prévôt²,

Thierry³

2009

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Although PEGylation of liposomes is used to reduce fusion, it does not completely eliminate it,177,178 and aggregation and fusion may still be a problem with PEGylated liposomes. In fact, under certain conditions, PEGylation may even increase fusion 179. Therefore, from this point of view, vesicles made from bolaamphiphiles that do not easily fuse may be superior to PE-Gylated liposomes.…”

Section: Bolaamphiphiles and Nano-structures For Drug Deliverymentioning

confidence: 99%

Lipid-Based Nanoparticles as Pharmaceutical Drug Carriers: From Concepts to Clinic

Puri

Loomis

Smith

et al. 2009

Crit Rev Ther Drug Carrier Syst

881

559

View full text Add to dashboard Cite

In recent years, various nanotechnology platforms in the area of medical biology, including both diagnostics and therapy, have gained remarkable attention. Moreover, research and development of engineered multifunctional nanoparticles as pharmaceutical drug carriers have spurred exponential growth in applications to medicine in the last decade. Design principles of these nanoparticles, including nano-emulsions, dendrimers, nano-gold, liposomes, drug-carrier conjugates, antibody-drug complexes, and magnetic nanoparticles, are primarily based on unique assemblies of synthetic, natural, or biological components, including but not limited to synthetic polymers, metal ions, oils, and lipids as their building blocks. However, the potential success of these particles in the clinic relies on consideration of important parameters such as nanoparticle fabrication strategies, their physical properties, drug loading efficiencies, drug release potential, and, most importantly, minimum toxicity of the carrier itself. Among these, lipid-based nanoparticles bear the advantage of being the least toxic for in vivo applications, and significant progress has been made in the area of DNA/RNA and drug delivery using lipid-based nanoassemblies. In this review, we will primarily focus on the recent advances and updates on lipid-based nanoparticles for their projected applications in drug delivery. We begin with a review of current activities in the field of liposomes (the so-called honorary nanoparticles), and challenging issues of targeting and triggering will be discussed in detail. We will further describe nanoparticles derived from a novel class of amphipathic lipids called bolaamphiphiles with unique lipid assembly features that have been recently examined as drug/DNA delivery vehicles. Finally, an overview of an emerging novel class of particles (based on lipid components other than phospholipids), solid lipid nanoparticles and nanostructured lipid carriers will be presented. We conclude with a few examples of clinically successful formulations of currently available lipid-based nanoparticles.

show abstract

“…Such interaction has been typically classified into four processes, namely adsorption, endocytosis, lipid exchange and fusion [19]. The latter phenomenon has been studied in detail by many authors because it offers the possibility for introducing drugs inside cells more easily [20][21][22].…”

Section: Page 5 Of 32mentioning

confidence: 99%

Encapsulation in fusogenic liposomes broadens the spectrum of action of vancomycin against Gram-negative bacteria

Nicolosi¹,

Scalia²,

Nicolosi³

et al. 2010

International Journal of Antimicrobial Agents

119

111

View full text Add to dashboard Cite

International audienceMany antibacterial agents, including the glycopeptides, are inactive against Gram-negative bacteria because of their inability to cross the outer membrane of these cells. Different chemical and technological approaches have been described to circumvent such limitation. In this study, we aimed to apply the strategy of fusogenic liposomes, up to now used to carry biological compounds and materials inside cells, to localise a glycopeptide antibiotic, vancomycin (VAN), to the periplasmic space, thus allowing it to exert its bactericidal activity. Small unilamellar liposome vesicles were prepared by an extrusion procedure (SUVETs) from a phospholipid-cholesterol hemisuccinate mixture known for its fusogenic properties with the eukaryotic cell membrane. VAN was loaded with high efficiency into these vesicles and in microbiological experiments in vitro was shown to be able to inhibit to a different extent the growth of wild and standard Gram-negative bacterial strains. Minimum inhibitory concentrations as low as 6mg/L were observed, for instance against clinical isolates of and . In comparison, neither the free antibiotic nor VAN-loaded 'classical' (non-fusogenic) liposomes showed any activity against the same bacteria. Scanning and transmission electron microscopy studies allowed confirmation that the produced SUVETs were able to adhere to and fuse with the external membrane of . According to preliminary experiments, this technological strategy can be proposed as a potentially successful way to enlarge the spectrum of activity of VAN

show abstract

Fusion and endocytosis of anionic liposomes with Ehrlich ascitic carcinoma cells

Cited by 10 publications

References 12 publications

Physico-Chemical Characteristics of Lipoplexes Influence Cell Uptake Mechanisms and Transfection Efficacy

Physico-Chemical Characteristics of Lipoplexes Influence Cell Uptake Mechanisms and Transfection Efficacy

Lipid-Based Nanoparticles as Pharmaceutical Drug Carriers: From Concepts to Clinic

Encapsulation in fusogenic liposomes broadens the spectrum of action of vancomycin against Gram-negative bacteria

Contact Info

Product

Resources

About