Polyethylene glycol 300 and low pH of the incubation medium initiate fusion of anionic lipid liposome membranes with Ehrlich ascitic carcinoma cell membranes. Some liposomes are endocytosed in cells and are distributed near the inner surface of the membrane. Liposome membranes spontaneously fuse with the cells under certain conditions.

We studied the effect of a new generation hybrid antioxidant IKhFAN-10 on the structure and function of cell membranes (organization of the lipid bilayer and proteins and activity of ion channels). The test preparation proposed for the therapy of neurodegenerative diseases modified properties of membranes in erythrocytes and Ehrlich ascites carcinoma cells and affected functional activity of cells. We determined the doses of this antioxidant, which did not cause side effects.

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The structure of sarcoplasmic reticulum membranes was studied in the presence of modeled transmembrane Ca2+ gradient corresponding to the status of Ca2+ depot at different stages of the muscle contraction-relaxation cycle in health and disease. Various sites of the membrane were characterized using spectral analysis of tryptophan, pyrene, and merocyanine-540 fluorescence without evaluating specific changes in the molecules of membrane components (Ca2+ -ATPase, ryanodine receptor, and lipids). The transmembrane Ca2+ gradient modulates the protein-lipid interactions and structural characteristics of the membrane. The proposed model can be used for studies of the effects of pharmacologically active substances and endogenous regulators.