Fusion plasmid carrying the colistin resistance gene mcr of Escherichia coli isolated from healthy residents

Hoa, Hoang Thi Thanh; Higashi, Ayano; Yamaguchi, Takahiro; Kawahara, Ryuji; Calvopiña, Manuel; Bastidas-Caldes, Carlos; Yamamoto, Masayuki; Yamamoto, Yoshimasa

doi:10.1016/j.jgar.2022.06.007

Cited by 5 publications

(12 citation statements)

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“…There was no correlation between phenotypic resistance and the presence of mcr-1 . This finding has been previously described by others ( 29 , 30 ), and it may be explained by the multifactorial aspects of antimicrobial resistance, such as (i) the diversity of plasmid types carrying mcr-1 ( 31 ), (ii) strong or weak plasmid promotors influencing the expression of the genes ( 32 ), and (iii) the fusion plasmid phenomenon that increases the transfer capabilities of mcr genes between hosts ( 9 ). Only four bacterial isolates were resistant, and all were found in pigs.…”

Section: Discussionsupporting

confidence: 69%

“…Colistin-resistant Enterobacterales, particularly E. coli and K. pneumoniae , carrying the mcr-1 gene have since been reported worldwide, including in the Americas (in Argentina, Bolivia [Plurinational State of], Brazil, Colombia, Peru and the United States) ( 8 ). Studies in Latin America have suggested that this spread might be associated with the horizontal transfer of colistin-resistance genes ( 9 ). Since the discovery of the mcr-1 gene and its presence in Enterobacterales plasmids, worldwide surveillance of colistin resistance has been strengthened ( 5 ).…”

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confidence: 99%

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Colistin resistance in Escherichia coli and Klebsiella pneumoniae in humans and backyard animals in Ecuador

Bastidas-Caldes

Guerrero-Freire

Ortuño-Gutiérrez³

et al. 2023

Revista Panamericana De Salud Pública

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Objective. Colistin is an antibiotic of last resort for treating serious Gram-negative bacterial infections. However, the misuse of colistin, especially as an animal growth promoter, has contributed to increasing antimicrobial resistance, mediated mainly through plasmid transfer of the mcr-1 gene. This study assessed the prevalence of phenotypic and molecular colistin resistance in Escherichia coli and Klebsiella pneumoniae in Ecuador in healthy humans and their chickens and pigs. Methods. Fecal samples were collected from humans and their chickens and pigs in two rural coastal and Amazon regions between April and August 2020. Gram-negative bacteria were isolated and identified using conventional techniques. Phenotypic resistance was determined using the broth microdilution technique, and the mcr-1 gene was detected using conventional polymerase chain reaction. Results. A total of 438 fecal samples were obtained from 137 humans, 147 pigs and 154 chickens. The prevalence of E. coli isolates was 86.3% (378/438) and K. pneumoniae, 37.4% (164/438). Overall, the mcr-1 gene was found in 90% (340/378) of E. coli isolates, with higher prevalences found in isolates from coastal regions (96.5%, 191/198), humans (95.6%, 111/116) and chickens (91.8%, 123/134); for K. pneumoniae, the gene was found in 19.5% (32/164) of isolates, with equal distribution between regions and hosts. Only four isolates, two E. coli and two K. pneumoniae, showed phenotypic resistance: mcr-1 was present in both E. coli strains but absent in the K. pneumoniae strains. Conclusions. Despite a low prevalence of phenotypic resistance to colistin, the high prevalence of the mcr-1 gene in E. coli is of concern. Ecuador’s ban on using colistin in animal husbandry must be enforced, and continual monitoring of the situation should be implemented.

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Section: Discussionsupporting

confidence: 69%

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confidence: 99%

Colistin resistance in Escherichia coli and Klebsiella pneumoniae in humans and backyard animals in Ecuador

Bastidas-Caldes

Guerrero-Freire

Ortuño-Gutiérrez³

et al. 2023

Revista Panamericana De Salud Pública

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“…Recently, two mcr-1 -carrying hybrid plasmids were characterized in two E . coli isolates recovered from stool specimens of healthy residents from Ecuador [ 16 ]. These two hybrid plasmids, named pLR-06 and pLR-50 (260,770 bp and 197,729 bp, respectively), harbored the mcr-1 .…”

Section: Resultsmentioning

confidence: 99%

“…These two hybrid plasmids, named pLR-06 and pLR-50 (260,770 bp and 197,729 bp, respectively), harbored the mcr-1 . 1 allele and with another antimicrobial resistance gene combinations [ 16 ]. To the best of our knowledge, our work constitutes the first worldwide report of an mcr-1 -containing hybrid FIA/HI1A/HI1B plasmid recovered from an isolate causing human infection.…”

Section: Resultsmentioning

confidence: 99%

“…In particular, the largest mcr-1 -harboring plasmids described to date belong to IncHI2A, with sequence lengths around 270 kb [ 15 ]. Finally, hybrid plasmids containing replicases belonging to more than one incompatibility group are rare, and are likely a consequence of IS-mediated fusions or homologous recombination events [ 16 , 17 ]. The presence of multiple incompatibility groups increases the host range of each single hybrid plasmid, rising the dissemination potential of antimicrobial resistance [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

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New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates

Martino,

Petroni,

Menocal

et al. 2024

PLoS ONE

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Mobile colistin resistance (mcr) genes were described recently in Gram-negative bacteria including carbapenem-resistant Enterobacterales. There are ten mcr genes described in different Gram-negative bacteria, however, Escherichia coli harboring mcr-1 gene is by far the most frequent combination. In Argentina, mcr-1 gene was characterized only on plasmids belonging to IncI2 group. The aim of this work was to get new insights of mcr-1-harboring plasmids from E. coli. Eight E. coli isolates from a larger collection of 192 clinical E. coli isolates carrying the mcr-1 gene were sequenced using next generation technologies. Three isolates belonged to ST131 high-risk clone, and five to single ST, ST38, ST46, ST226, ST224, and ST405. Eight diverse mcr-1-harboring plasmids were analyzed: IncI2 (1), IncX4 (3), IncHI2/2A (3) and a hybrid IncFIA/HI1A/HI1B (1) plasmid. Plasmids belonging to the IncI2 (n = 1) and IncX4 (n = 3) groups showed high similarity with previously described plasmids. Two IncHI2/HI2A plasmids, showed high identity between them, while the third, showed several differences including additional resistance genes like tet(A) and floR. One IncFIA/H1A/H1B hybrid plasmid was characterized, highly similar to pSRC27-H, a prototype plasmid lacking mcr genes. mcr-1.5 variant was found in four plasmids with three different Inc groups: IncI2, IncHI2/HI2A and the hybrid FIA/HI1A/HI1B plasmid. mcr-1.5 variant is almost exclusively described in our country and with a high frequency. In addition, six E. coli isolates carried three allelic variants codifying for CTX-M-type extended-spectrum-β-lactamases: blaCTX-M-2 (3), blaCTX-M-65 (2), and blaCTX-M-14 (1). It is the first description of mcr-1 harboring plasmids different to IncI2 group in our country. These results represents new insights about mcr-1 harboring plasmids recovered from E. coli human samples from Argentina, showing different plasmid backbones and resistance gene combinations.

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Chromosomal qnrB19 -carrying Escherichia coli isolated from the stool sample of a community resident in Ecuador

Hoang,

Yamamoto,

Calvopina

et al. 2024

Microbiol Resour Announc

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Fusion plasmid carrying the colistin resistance gene mcr of Escherichia coli isolated from healthy residents

Cited by 5 publications

References 5 publications

Colistin resistance in Escherichia coli and Klebsiella pneumoniae in humans and backyard animals in Ecuador

Colistin resistance in Escherichia coli and Klebsiella pneumoniae in humans and backyard animals in Ecuador

New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates

Chromosomal qnrB19 -carrying Escherichia coli isolated from the stool sample of a community resident in Ecuador

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