G and P genotypes of group A rotavirus strains circulating in calves in Brazil, 1996–1999

Alfieri, Alice Fernandes; Barreiros, Maria Alexandra; Leite, José Paulo Gagliardi; Richtzenhain, Leonardo José

doi:10.1016/j.vetmic.2003.10.029

Cited by 72 publications

(76 citation statements)

References 29 publications

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“…In the present study, consensus primers were used to amplify the partial-length copies of the rotavirus VP4 gene which yielded the expected amplicon size of 876 bp and full length copies of VP7 gene, which yielded 897 bp product in all the nine field samples, which in turn was used as a template in multiplex PCR to identify all the possible G and P genotype present in bovine rotavirus strains as reported by [12].…”

Section: Discussionmentioning

confidence: 94%

Genotyping of Rotavirus in Neonatal Calves with Acute Gastroenteritis in Iraq

Abdulazeez¹,

Abed²

2017

AiM

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Globally, Rotavirus is the common major etiologic agents of diarrhea in infant, young children and neonatal calves. It is very important to early diagnose the disease for effective treatment. The objective of this study was to determine the prevalence, molecular characteristics, and the effect of rotavirus strains for severe gastroenteritis in neonatal calves in five Iraqi governorates (Al-Qadissiya, Babel, Kerbala, Missan, Wassit). A total of 125-stool specimens were examined, it have been collected from calves form the period between November 2015 to March 2016. The ages were ranging from 6 to 60 weeks. The specimens were examined using Chromatographic Immunoassay, enzyme-linked immunosorbent assay (ELISA) and Polymerase-chain reaction (PCR). Our results gave us 67 (53.6%) positive by chromatographic immunoassay, 45 (36%) positive by ELISA and 32 (25.6%) positive by PCR. Genotyping were analyzed by multiplex PCR. Genotype combination G1P . Such information will not only aid in seeking advocacy for introducing rotavirus vaccine in national immunization program in Iraq, but will also help in the evaluation of the efficacy of these vaccines in relation to the rotavirus genotyping circulation.

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Section: Discussionmentioning

confidence: 94%

Genotyping of Rotavirus in Neonatal Calves with Acute Gastroenteritis in Iraq

Abdulazeez¹,

Abed²

2017

AiM

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“…After homogenization, the samples were incubated at 56 o C for 30 min. For DNA extraction a combination of phenol/chloroform/isoamyl alcohol and silica/ guanidine isothiocyanate methods was performed according to Alfieri et al (2004). First, the samples were treated with an equal volume of phenol/chloroform/isoamyl alcohol (25:24:1), homogenized, and heated at 56 o C for 15 min (Sambrook & Russell 2001).…”

Section: Methodsmentioning

confidence: 99%

Bovine papillomavirus type 2 detection in the urinary bladder of cattle with chronic enzootic haematuria

Wosiacki

Claus

Alfieri

2006

Mem. Inst. Oswaldo Cruz

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“…The temporal and geographical distribution and diversity of G8 strains seen before 2009 may suggest multiple zoonotic introductions [13,[27][28][29][30][31][32][33] whereas the similarity of the strains described in the current paper is likely to be the result of sustained person-to-person transmission of a strain adapted to the human host (Fig. 5).…”

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confidence: 99%

Rotavirus genotypes co-circulating in Europe between 2006 and 2009 as determined by EuroRotaNet, a pan-European collaborative strain surveillance network

Iturriza‐Gómara¹,

Dallman²,

Bányai³

et al. 2010

Epidemiol. Infect.

207

254

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SUMMARYEuroRotaNet, a laboratory network, was established in order to determine the diversity of co-circulating rotavirus strains in Europe over three or more rotavirus seasons from 2006/2007 and currently includes 16 countries. This report highlights the tremendous diversity of rotavirus strains co-circulating in the European population during three years of surveillance since 2006/ 2007 and points to the possible origins of these strains including genetic reassortment and interspecies transmission. Furthermore, the ability of the network to identify strains circulating with an incidence of o1% allowed the identification of possible emerging strains such as G8 and G12 since the beginning of the study ; analysis of recent data indicates their increased incidence.

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G and P genotypes of group A rotavirus strains circulating in calves in Brazil, 1996–1999

Cited by 72 publications

References 29 publications

Genotyping of Rotavirus in Neonatal Calves with Acute Gastroenteritis in Iraq

Genotyping of Rotavirus in Neonatal Calves with Acute Gastroenteritis in Iraq

Bovine papillomavirus type 2 detection in the urinary bladder of cattle with chronic enzootic haematuria

Rotavirus genotypes co-circulating in Europe between 2006 and 2009 as determined by EuroRotaNet, a pan-European collaborative strain surveillance network

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