G Protein-Coupled Receptors as Target Sites for Insecticide Discovery

Bai, Hua; Palli, Subba Reddy

doi:10.1007/978-94-007-4497-4_4

Cited by 13 publications

(13 citation statements)

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“…We therefore searched the L. migratoria genome for insecticide gene targets. We identified 34 cys-loop ligand-gated ion channels and 90 G-protein-coupled receptors, which are considered to be major traditional insecticide targets 54 55 ( Supplementary Note 3 and Supplementary Table S35 ). Using in-silico screening 56 for L. migratoria orthologues of Caenorhabditis elegans and D. melanogaster lethal genes (linked to lethal phenotypes on gene perturbation), we identified 166 genes that may be worth considering for targets in alternative pest control strategies given that they are single copy, have lower allelic variability and lack similarity to human genes.…”

Section: Resultsmentioning

confidence: 99%

The locust genome provides insight into swarm formation and long-distance flight

Fang²,

et al. 2014

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Locusts are one of the world’s most destructive agricultural pests and represent a useful model system in entomology. Here we present a draft 6.5 Gb genome sequence of Locusta migratoria, which is the largest animal genome sequenced so far. Our findings indicate that the large genome size of L. migratoria is likely to be because of transposable element proliferation combined with slow rates of loss for these elements. Methylome and transcriptome analyses reveal complex regulatory mechanisms involved in microtubule dynamic-mediated synapse plasticity during phase change. We find significant expansion of gene families associated with energy consumption and detoxification, consistent with long-distance flight capacity and phytophagy. We report hundreds of potential insecticide target genes, including cys-loop ligand-gated ion channels, G-protein-coupled receptors and lethal genes. The L. migratoria genome sequence offers new insights into the biology and sustainable management of this pest species, and will promote its wide use as a model system.

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Section: Resultsmentioning

confidence: 99%

The locust genome provides insight into swarm formation and long-distance flight

Fang²,

et al. 2014

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“…By means of manipulating arrestin-2 and in turn the associated signal transduction pathway, it is anticipated that the insect development and behavior could be disrupted. For instance, Bai et al (2011Bai et al ( , 2013 have reported the potential of novel pesticide development by targeting GPGR-associated protein genes. Furthermore, the RNA interference pathway was shown to be capable of inducing larval lethality in coleopterans through intake of dsRNA expressed in the host plants (Baum et al 2007), and this technique may be applied to inhibit the expression of arrestin-2 in M. vitrata.…”

Section: Discussionmentioning

confidence: 99%

Molecular-Phylogenetic Characterization of Arrestin-2 from Maruca vitrata (Lepidoptera: Crambidae)

Chang

Srinivasan

2013

Annals of the Entomological Society of America

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Most physiological processes to external stimulants rely upon intracellular signal transduction mediated by ligand binding of G-protein-coupled receptors. T'he C-protein signaling pathway is deactivated by arrestin-2 binding, which is indispensable for receptor intemalization and recycling. We identified the full-length cDNA encoding arrestin-2 in Maruca vitrata F. using rapid amplification of cDNA ends. The open reading frame of MaviArr2 is 1,221 bp in length, encoding 407 amino acids. The alignment of the known arrestin-2 amino acid sequences from other insects revealed that MaviArr2 has the highest similarity (98.8%) to the monarch butterfiy, but shows low homology (53.9-60.7%) with ants and bees. A certain number of highly conserved protein-binding motifs were identified from the deduced MaviArr2 amino acids, demonstrating their function as receptor deactivators in signal transduction pathways in cells. The genomic DNA sequence of MaviArr2, amplified by polymerase chain reaction, is 1,779 bp in size and is composed of six exons. Real-time quantitative polymerase chain reaction assay demonstrated a relatively higher expression of MaviArr2 mRNA in the late pupal stages, suggesting multiple developmental functions. Phylogenetic analysis showed the lepidopteran arrestin-2 protein sequence is closely related to that of Diptera, but distant from Hymenoptera arrestin-2. Intraspecific genomic sequence comparisons of MaviArr2 show a greater conservation ofthe gene in M vitrata from Africa than those from geographical locations in Asia. These findings are a significant step forward in our understanding of arrestin-2 gene architecture and functions, which may provide a possibility to manage M. vitrata through molecular and phylogenetic techniques.

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“…4 by Bai and Palli 2012 , this book) but, paradoxically, this class of receptors has received relatively little attention as targets for insecticide action (Van Hiel et al 2010 ) . In many cases, activation of GPCRs results in the production of intracellular messengers such as cAMP and calcium for which fl uorescent and luminescent detection methods are readily available for screening in high-throughput format (Milligan 2003 ;Williams 2004 ) .…”

Section: G Protein-coupled Receptors (Gpcrs)mentioning

confidence: 96%