G1-4 A, an arabinogalactan polysaccharide from Tinospora cordifolia increases dendritic cell immunogenicity in a murine lymphoma model

Pandey, Vipul K.; Shankar, Bhavani S.; Sainis, Krishna B.

doi:10.1016/j.intimp.2012.09.020

Cited by 31 publications

(22 citation statements)

References 45 publications

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“…Tinospora cordifolia has been shown to have antiproliferative activity in various hepato-carcinoma, lymphoma, and bone cancer cell lines [11,30]. Anti-proliferative property of TCE may be the result of induction of differentiation and senescence as depicted by enhanced expression of GFAP which plays an important role in maintaining the normal astrocyte morphology and growth [58,59].…”

Section: Discussionmentioning

confidence: 99%

“…This plant has been used as remedy for jaundice and its extracts and purified components have been found to have hepato-protective effect against various toxic agents [3,4]. The crude extract and various compounds isolated from this plant have been reported to have several medicinal properties [5] including immunomodulatory [6] and immunostimulatory activity [7,8] that helps in increasing immune response by the lymphocytic cells [9], macrophages [10] and dendritic cells [11]. Several recent reports have suggested that the plant extract is a rich source of biochemicals that have potential therapeutic value in treating diabetes and related disorders caused by disturbed carbohydrate metabolism [12–17].…”

Section: Introductionmentioning

confidence: 99%

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Aqueous Ethanolic Extract of Tinospora cordifolia as a Potential Candidate for Differentiation Based Therapy of Glioblastomas

Mishra

Kaur

2013

PLoS ONE

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Glioblastomas are the most aggressive primary brain tumors and their heterogeneity and complexity often renders them non responsive to various conventional treatments. Search for herbal products having potential anti-cancer activity is an active area of research in the Indian traditional system of medicine i.e., Ayurveda. Tinospora cordifolia, also named as ‘heavenly elixir’ is used in various ayurvedic decoctions as panacea to treat several body ailments. The current study investigated the anti-brain cancer potential of 50% ethanolic extract of Tinospora cordifolia (TCE) using C6 glioma cells. TCE significantly reduced cell proliferation in dose-dependent manner and induced differentiation in C6 glioma cells, resulting in astrocyte-like morphology as indicated by phase contrast images, GFAP expression and process outgrowth data of TCE treated cells which exhibited higher number and longer processes than untreated cells. Reduced proliferation of cells was accompanied by enhanced expression of senescence marker, mortalin and its translocation from perinuclear to pancytoplasmic spaces. Further, TCE showed anti-migratory and anti-invasive potential as depicted by wound scratch assay and reduced expression of plasticity markers NCAM and PSA-NCAM along with MMP-2 and 9. On analysis of the cell cycle and apoptotic markers, TCE treatment was seen to arrest the C6 cells in G0/G1 and G2/M phase, suppressing expression of G1/S phase specific protein cyclin D1 and anti-apoptotic protein Bcl-xL, thus supporting its anti-proliferative and apoptosis inducing potential. Present study provides the first evidence for the presence of anti-proliferative, differentiation-inducing and anti-migratory/anti-metastatic potential of TCE in glioma cells and possible signaling pathways involved in its mode of action. Our primary data suggests that TCE and its active components may prove to be promising phytotherapeutic interventions in gliobalstoma multiformae.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Aqueous Ethanolic Extract of Tinospora cordifolia as a Potential Candidate for Differentiation Based Therapy of Glioblastomas

Mishra

Kaur

2013

PLoS ONE

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“…For example, G1-4a, an arabinogalactan isolated from T. cordifolia that has a chemical structure similar to IIP-2, was shown to activate bone marrow-derived dendritic cells (BMDCs) in vitro and spleen dendritic cells in vivo. Secretion of the cytokines IL-12 and TNF-␣ from BMDCs and T cell stimulation also increased [29]. In the current study, we evaluated antigen-specific cellular immune responses by ELISpot and ICS.…”

Section: Discussionmentioning

confidence: 98%

“…Its structure and activity as an adjuvant were previously described [28]. Previous studies have also shown that arabinogalactan can be used either to potentiate immune responses or as an adjuvant in human or animal vaccines [29][30][31].…”

Section: Introductionmentioning

confidence: 96%

Isatis indigotica root polysaccharides as adjuvants for an inactivated rabies virus vaccine

Zhang

Zheng

Cheng

et al. 2016

International Journal of Biological Macromolecules

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Adjuvants can enhance vaccine immunogenicity and induce long-term enhancement of immune responses. Thus, adjuvants are important for vaccine research. Polysaccharides isolated from select Chinese herbs have been demonstrated to possess various beneficial functions and excellent adjuvant abilities. In the present study, the polysaccharides IIP-A-1 and IIP-2 were isolated from Isatis indigotica root and compared with the common vaccine adjuvant aluminum hydroxide via intramuscular co-administration of inactivated rabies virus rCVS-11-G into mice. Blood was collected to determine virus neutralizing antibody (VNA) titers and B and T lymphocyte activation status. Inguinal lymph node samples were collected and used to measure B lymphocyte proliferation. Splenocytes were isolated, from which antigen-specific cellular immune responses were detected via ELISpot, ELISA and intracellular cytokine staining. The results revealed that both types of polysaccharides induce more rapid changes and higher VNA titers than aluminum hydroxide. Flow cytometry assays revealed that the polysaccharides activated more B lymphocytes in the lymph nodes and more B and T lymphocytes in the blood than aluminum hydroxide. Antigen-specific cellular immune responses showed that IIP-2 strongly induced T lymphocyte proliferation in the spleen and high levels of cytokine secretion from splenocytes, whereas aluminum hydroxide induced proliferation in only a small number of lymphocytes and the secretion of only small quantities of cytokines. Collectively, these data suggest that the polysaccharide IIP-2 exhibits excellent adjuvant activity and can enhance both cellular and humoral immunity.

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“…Merr., enhanced the maturation and function of BM-DCs, and the secretion of IL-12 and TNF-α. Administration of tumor lysate pulsed G1-4A (2 mg/ml) treated DC resulted in decreased tumor burden in preventive as well as therapeutic tumor challenge experiments in a murine lymphoma model (Pandey et al, 2012). Angelan, a nature TLR4 ligand, promoted the maturation of DCs and increased the expression of IL-12 and CCR7 through NF-κB and p38 pathway and enhanced their migration against CCL19 (J.Y.…”

Section: The Adjuvant Effects Of Chm On Immune Responsesmentioning

confidence: 99%

The immunoregulatory effects of Chinese herbal medicine on the maturation and function of dendritic cells

Zhang

2015

Journal of Ethnopharmacology

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G1-4 A, an arabinogalactan polysaccharide from Tinospora cordifolia increases dendritic cell immunogenicity in a murine lymphoma model

Cited by 31 publications

References 45 publications

Aqueous Ethanolic Extract of Tinospora cordifolia as a Potential Candidate for Differentiation Based Therapy of Glioblastomas

Aqueous Ethanolic Extract of Tinospora cordifolia as a Potential Candidate for Differentiation Based Therapy of Glioblastomas

Isatis indigotica root polysaccharides as adjuvants for an inactivated rabies virus vaccine

The immunoregulatory effects of Chinese herbal medicine on the maturation and function of dendritic cells

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