G6PC3, ALDOA and CS induction accompanies mir-122 down-regulation in the mechanical asphyxia and can serve as hypoxia biomarkers

Zeng, Yan; Lv, Yubao; Li, Tao; Ma, Jun; Zhang, Heng; Xu, H. C.; Bi, Xiao; Shi, Qun; Ma, Kaijun; Chen, Long

doi:10.18632/oncotarget.12931

Cited by 38 publications

(22 citation statements)

References 31 publications

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“…In addition, we found a strong and direct correlation between liver injury and miR−122-5p (r = 0.91, p < 0.0001), which confirms previous observations by others (49,50), and which can be explained not only by passive release but also by the augmentation of miR-122 in the liver. MiR-122 was also found to play a role in the hypoxia responses that regulate glucose and energy metabolism (51). In addition, it was recently reported that ER stress impacts on miR-122 promoter activity (52).…”

Section: Discussionmentioning

confidence: 99%

Circulating miRNAs Associated With ER Stress and Organ Damage in a Preclinical Model of Trauma Hemorrhagic Shock

et al. 2020

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Section: Discussionmentioning

confidence: 99%

Circulating miRNAs Associated With ER Stress and Organ Damage in a Preclinical Model of Trauma Hemorrhagic Shock

et al. 2020

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“…For example, miR-101 expression protects cardiac fibroblasts from hypoxia-induced apoptosis [43]. In a rat hypoxia model, miR-122 and its target genes play roles in the hypoxia responses that regulate glucose and energy metabolism and serve as potential biomarkers of mechanical asphyxia [44]. All these results support the concept that miRNAs regulate the response to hypoxia.…”

Section: Discussionmentioning

confidence: 89%

MiR-19b-3p Regulates MAPK1 Expression in Embryonic Fibroblasts from the Great Tit (Parus Major) Under Hypoxic Conditions

Chen

Cheng

et al. 2018

Cell Physiol Biochem

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Background/Aims: Genomic adaptations to high altitudes have been well studied in the last several years; however, the roles of microRNAs (miRNAs), which are essential modulators of a variety of genes and key cellular processes, have rarely been explored. Here, we explored the interactions between miRNAs and their target genes as an adaptation to high altitude in an avian species, the great tit (Parus major), which is widely distributed across the Eurasian continent at altitudes between 4500 m and sea level. Because the MAPK signaling pathway plays a crucial role in the hypoxia response in the great tit, we chose MAPK1 as a target candidate gene. Methods: We established a great tit embryonic fibroblast line and subsequently studied the relationship between miRNA-19b-3p and MAPK1 in normoxia and hypoxia groups. Meanwhile, the great tit embryonic fibroblasts (GEFs) were treated or transfected with miR-19b-3p mimics, inhibitors, or si-MAPK1, and their proliferation was subsequently assessed using the MTT assay. The expression of the miRNAs and MAPK1 was measured by real-time PCR and Western blotting. Results: We identified 14 miRNAs in the cardiac tissues of great tits that are related to hypoxia adaptation. MAPK1 binds only to miR-19b-3p of the 14 miRNAs predicted by both TargetScan and miRanda software. Specifically, we validated the computational prediction of miR-19b-3p binding to the 3’UTR of MAPK1 using a luciferase reporter assay. Our results show that miR-19b-3p promotes GEFs proliferation and up-regulates MAPK1 expression. Moreover, miR-19b-3p mimics and MAPK1 knockdown induce GEFs apoptosis and regulate the cell cycle under hypoxic conditions. Conclusions: Our study is the first to describe an important miRNA-mediated regulatory mechanism of high altitude adaptation in a non-model wild songbird and highlights the importance of studies on miRNA-mediated mechanisms of hypoxic adaptations in other animals.

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“…As a catalytic enzyme, ALDOA represents one of the key enzymes in the glycolysis process. Of note, it plays a role in the hypoxia responses regulating both glucose and energy metabolism and can serve as hypoxia biomarkers [41]. Ischemic stroke represents a typical atherosclerosis-related disease.…”

Section: Discussionmentioning

confidence: 99%

Association of serum levels of antibodies against ALDOA and FH4 with transient ischemic attack and cerebral infarction

Wang¹,

Lü²,

Zhang

et al. 2020

Preprint

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Background and Purpose: Ischemic stroke, such as Transient ischemic attack (TIA) and cerebral infarction (CI) , are the serious problems in the aging society. Therefore, development of biomarkers for TIA and CI is attempted.Methods: Candidate antigens recognized by IgG autoantibodies in the sera of nineteen TIA patients were screened by a human aortic endothelial cell cDNA library. Serum antibody levels against the antigens were examined by amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) in healthy donor (HD), TIA, and CI cohorts (n = 285, 92 and 529). A case-control study nested within the Japan Public Health Center-based Prospective Cohort Study (JPHC) was performed.Results: Aldolase A, fructose-bisphosphate (ALDOA) and fumarate hydratase (FH) were identified as the candidate antigens. AlphaLISA revealed that anti-ALDOA and anti-FH antibody levels were both higher in TIA or CI patients than in HDs ( P < 0.0001). The levels of anti-ALDOA [Odds ratio (OR): 2.46, P = 0.005] and anti-FH (OR: 2.49, P = 0.0037) were independent predictors of TIA by multivariate logistic regression analysis, similar results were found in CI. The case-control study showed the levels of anti-ALDOA (OR: 2.50, P < 0.01) and anti-FH (OR: 2.60, P < 0.01) were associated with risk of CI.Spearman's correlation analysis demonstrated an association between the anti-ALDOA and anti-FH levels and risk factors of ischemic stroke, such as age, smoking habit, coronary heart disease, and hypertension.Conclusions: Anti-ALDOA and anti-FH antibodies can serve as novel potential biomarkers for prediction of TIA and CI.

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G6PC3, ALDOA and CS induction accompanies mir-122 down-regulation in the mechanical asphyxia and can serve as hypoxia biomarkers

Cited by 38 publications

References 31 publications

Circulating miRNAs Associated With ER Stress and Organ Damage in a Preclinical Model of Trauma Hemorrhagic Shock

Circulating miRNAs Associated With ER Stress and Organ Damage in a Preclinical Model of Trauma Hemorrhagic Shock

MiR-19b-3p Regulates MAPK1 Expression in Embryonic Fibroblasts from the Great Tit (Parus Major) Under Hypoxic Conditions

Association of serum levels of antibodies against ALDOA and FH4 with transient ischemic attack and cerebral infarction

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