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Abstract

This study was carried out to clarify the toxicity properties of Flower-like zinc oxide nanoparticles (ZnO NPs) on collected sperm samples from Cyprinus carpio using computer-assisted sperm analysis (CASA). Flower-like ZnO NPs were successfully synthesized using a simple system at 120• C for 5 h by the microwave-assisted solvothermal techniques. The synthesized ZnO NPs were characterized for its structural, morphological and thermal properties. After that, the different concentrations of synthesized ZnO NPs (0.001, 0.01, 0.1, 0.5, 1 ppm) were examined with Cyprinus carpio sperm samples collected from Karakaya reservoir. The spermatozoa motility parameters which contain to curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), straightness (STR), amplitude of lateral head displacement (ALH), and beat cross frequency (BCF) was computed and recorded by CASA. According to the results, while VCL, VAP and BCF were statistically significant (P<0.05), VSL, ALH and STR were not statistically significant (P>0.05). In addition, the EC 50 values of VCL and VAP parameters were calculated 0.56 ppm and 0.004 ppm, respectively.

The Effect of Copper and Copper Oxide Nanoparticles on Rainbow Trout (Oncorhynchus mykiss W.) Spermatozoa Motility after Incubation with Contaminants

“…In study by Ozgur et al [45][46][47][48]62] the ALH was not affected by nanoproducts while the BCF increased in the highest concentrations of nanoproducts (when the short incubation time is considered). In our study, the factors tested also had no effect on ALH, while BCF increased and then decreased with increasing concentration of nanometals and extended incubation time.…”

“…So far, few studies have been conducted using nanometals. One of the earliest studies on the effects of nanometals on the motility of fish sperm was a study by Ozgur and coworkers [ 45 , 46 , 47 , 48 ]. The authors investigated various nanometals, concentrations, and exposure times.…”

“…The authors investigated various nanometals, concentrations, and exposure times. In Ozgur et al [ 48 ], sperm were incubated with the oxygenated form of a nanozinc (ZnO NPs). This study concerning 4 h incubation of Cyprinus carpio spermatozoon in ZnO NPs (at concentrations: (0.001, 0.01, 0.1, 0.5, 1 mg·L −1 ) showed a statistically significant increase in velocity (VCL) in the lowest dose of 0.001–0.01 mg·L −1 ZnO NPs while it significantly decreased in concentrations higher than 0.05 ppm.…”

“…In the studies by Ozgur et al [ 45 , 46 , 47 , 48 , 62 ], the percentage of motile sperm was not analysed. In the present study where rainbow trout spermatozoa were incubated with copper nanoproducts, lesser toxicity of CuO NPs than Cu NPs was seen.…”

“…There are few studies on the effect of NPs on fish spermatozoa. Ozgur et al [ 45 , 46 , 47 , 48 ] studied the effect of several nanoparticles including ZnO, TiO 2 , SiO 2 , and Fe 3 O 4 NPs on carp and rainbow trout spermatozoa motility parameters after 1 or 3–4 h of incubation with nanoproducts. There are two methods of studying the effects of pollutants on sperm: storage of immotile spermatozoa with pollutants for defined time periods (long-term contact), or direct interaction by placing the nanoproduct into an activation media that triggers motility (short-lived contact during motility phase) [ 41 , 49 , 50 , 51 , 52 , 53 , 54 ].…”