GABA and serotonin immunoreactivity during postembryonic brain development in the beetleTenebrio molitor

Wegerhoff, Rainer

doi:10.1002/(sici)1097-0029(19990501)45:3<154::aid-jemt3>3.0.co;2-5

Cited by 34 publications

(28 citation statements)

References 49 publications

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“…In the course of the postembryonic life, numerous nerve cells will be added to the already existing cell clusters. This kind of postembryonic neurogenesis has also been observed in both metamorphic (Cash and Carew, 1989;Croll and Chiasson, 1989;Booker et al, 1996;Wegerhoff, 1999;Voronezhskaya et al, 2003) and directly developing invertebrates (Solt and Molná r, 2001). During the postembryonic development the localization and division of cell groups will change together with the increasing volume of the ganglia.…”

Section: Embryogenesis Of Serotonergic Neurons In Eisenia Fetidamentioning

confidence: 69%

Embryogenesis of the serotonergic system in the earthworm Eisenia fetida (Annelida, Oligochaeta): Immunohistochemical and biochemical studies

Koza

Wilhelm

Hiripi

et al. 2006

J of Comparative Neurology

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Organization of the serotonergic system and changes of the serotonin (5-HT) content were studied during the embryogenesis of the earthworm Eisenia fetida, using immunocytochemistry and HPLC. A gradual emergence of 5-HT immunoreactive (IR) cells and their axon projections in the several ganglia of the central (CNS) and peripheral nervous system are described in the context of a staged time-scale of development. The first 5-HT-IR neurons appear in the subesophageal ganglion at an early embryonic stage (E2), followed by neurons in some rostrally located ventral ganglia. In the cerebral ganglion, 5-HT-IR cells can be detected only from stage E5. The number of labeled cells in each ganglion of the embryo increases until hatching, when it is still considerably lower than that observed in adults. This shows that the development of the 5-HTergic system is far from complete by the end of embryogenesis. Organization of 5-HT-IR innervation of the body wall starts by stages E3 to E4. In the stomatogastric nervous system the first 5-HT-IR fibers can be detected by stage E5. By stage E9 5-HT immunopositive neurons can be observed in both the stomatogastric ganglia and the enteric plexus. Both 5-HT levels and the numbers of the labeled cells show a significant increase before hatching, which indicate a functional maturation of the 5-HTergic system. Based on the early appearance of 5-HT, we suppose that it may play a regulatory role in both the gangliogenesis and the maturation of peripheral functions necessary during postembryonic life.

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Section: Embryogenesis Of Serotonergic Neurons In Eisenia Fetidamentioning

confidence: 69%

Embryogenesis of the serotonergic system in the earthworm Eisenia fetida (Annelida, Oligochaeta): Immunohistochemical and biochemical studies

Koza

Wilhelm

Hiripi

et al. 2006

J of Comparative Neurology

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“…(Enell et al 2007) or on larvae that move independently (Wegerhoff 1999). Dzitoyeva et al (2005) suggest that GABA receptors in Drosophila are required for normal larval development, but the participating molecular mechanisms in this process are still not clear.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, morphological features of epithelium degeneration, observed before the last larval instar (post-defecation period), are signs of perturbation induced by sublethal doses of chemical compounds or pathogenic agents in this larval organ (Gregorc and Bowen 1998, 1999, 2000Gregorc and Smodis-Skerl 2007). Malaspina and Silva-Zacarin (2006) proposed as an additional study to the in vitro toxicological bioassay, morphological and immunocytochemical analyses of honeybee organs of larvae, and adults exposed to pesticides in order to evaluate the risks to bees exposed to sublethal doses of pesticides in agroecosystems.…”

Section: Introductionmentioning

confidence: 99%

Morphological alterations induced by boric acid and fipronil in the midgut of worker honeybee (Apis mellifera L.) larvae

et al. 2009

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Morphological alterations, by means of histological and ultrastructural analysis, have been used to determine the effects of boric acid and fipronil on midgut tissues of honeybee worker, Apis mellifera L. larvae. In order to observe possible morphological alterations in the midgut, two groups of bioassays were performed. In the first one, the larvae were chronically treated with different concentrations of boric acid added to the food (1.0, 2.5 and 7.5 mg/g). In the second group, the larvae were fed with diets containing different concentrations of fipronil (0.1 and 1 microg/g) and compared with control groups without these chemical compounds. In the first bioassay, the larvae were collected on day 3 and in the second bioassay on day 4, when the mortality rate obtained in the toxicological bioassay was not very high. The larval midguts were removed and processed for morphological analyses using a light and transmission electron microscopy. We observed cytoplasmic vacuolizations, with the absence of autophagic vacuoles, and chromatinic compacting in most of the cells in the groups treated with pesticides. The morphological alterations were far greater in the larvae treated with boric acid than in the larvae treated with fipronil. Our data suggest that the midgut cell death observed was in response to boric acid and fipronil action. This study significantly improves the understanding of the toxicological effect of these insecticides from the ecotoxicological perspective.

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“…The distribution of serotonin has been extensively mapped in a number of arthropods (e.g., Myhrberg et al 1979;Beltz and Kravitz 1983;Bishop and O'Shea 1983;Elofsson 1983Elofsson , 1992Cournil et al 1984;Klemm et al 1984;Nässel and Elekes 1984;Schürmann and Klemm 1984;Nässel and Elekes 1985;Longley and Longley 1986;Siwicki et al 1987;Sandeman et al 1988;Dubbels and Elofsson 1989;Homberg and Hildebrand 1989;Katz et al 1989;Beltz et al 1990;Real and Czternasty 1990;Salecker and Distler 1990;Lundell and Hirsh 1994;Thompson et al 1994;Christie et al 1995;Harzsch and Dawirs 1995;Schürmann et al 1995;Würden and Homberg 1995;Bicker 1999;Callaway and Stuart 1999;Hörner 1999;Leitinger et al 1999;Tierney et al 1999;Wegerhoff 1999;Ignell 2001;Homberg 2002;Moreau et al 2002;Harzsch 2004;Settembrini and Villar 2004;Fu et al 2005;Gallus et al 2005;Barthelemy et al 2006;…”

Section: Copepod Cns Contains Extensive Aminergic Innervationmentioning

confidence: 99%

Immunohistochemical mapping of histamine, dopamine, and serotonin in the central nervous system of the copepod Calanus finmarchicus (Crustacea; Maxillopoda; Copepoda)

Hartline

Christie

2010

Cell Tissue Res

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Calanoid copepods constitute an important group of marine planktonic crustaceans that often dominate the metazoan biomass of the world's oceans. In proportion to their ecological importance, little is known about their nervous systems. We have used immunohistochemical techniques in a common North Atlantic calanoid to localize re-identifiable neurons that putatively contain the biogenic amines histamine, dopamine, and serotonin. We have found low numbers of such cells and cell groups (approximately 37 histamine pairs, 22 dopamine pairs, and 12 serotonin pairs) compared with those in previously described crustaceans. These cells are concentrated in the anterior part of the central nervous system, the majority for each amine being located in the three neuromeres that constitute the brain (protocerebrum, deutocerebrum, and tritocerebrum). Extensive histamine labeling occurs in several small compact protocerebral neuropils, three pairs of larger, more posterior, paired, dense neuropils, and one paired diffuse tritocerebral neuropil. The most concentrated neuropil showing dopamine labeling lies in the putative deutocerebrum, associated with heavily labeled commissural connections between the two sides of the brain. The most prominent serotonin neuropil is present in the anterior medial part of the brain. Tracts of immunoreactive fibers of all three amines are prominent in the cephalic region of the nervous system, but some projections into the most posterior thoracic regions have also been noted.

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GABA and serotonin immunoreactivity during postembryonic brain development in the beetleTenebrio molitor

Cited by 34 publications

References 49 publications

Embryogenesis of the serotonergic system in the earthworm Eisenia fetida (Annelida, Oligochaeta): Immunohistochemical and biochemical studies

Embryogenesis of the serotonergic system in the earthworm Eisenia fetida (Annelida, Oligochaeta): Immunohistochemical and biochemical studies

Morphological alterations induced by boric acid and fipronil in the midgut of worker honeybee (Apis mellifera L.) larvae

Immunohistochemical mapping of histamine, dopamine, and serotonin in the central nervous system of the copepod Calanus finmarchicus (Crustacea; Maxillopoda; Copepoda)

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