Gadolinium ion inhibits loss of metabolites induced by osmotic shock and large stretch‐activated channels in bacteria

Berrier, Catherine; Coulombe, Alain; Szabó, Ildikò; Zoratti, Mario; Ghazi, Alexandre

doi:10.1111/j.1432-1033.1992.tb16960.x

Cited by 200 publications

(194 citation statements)

References 31 publications

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“…Both gadolinium (Gdd3+) and amiloride have been shown to inhibit SA channels (Yang & Sachs, 1989;Berrier, Coulombe, Szabo, Zoratti & Ghazi, 1989;Hamill, Lane & MacBride, 1992 In pregnant rat uteri, the threshold excursion value of stretch applied in 0 5 s was -14% which was smaller than that of uterine muscle from the non-pregnant rat (-26%).…”

Section: Discussionmentioning

confidence: 99%

Stretch‐induced enhancement of contractions in uterine smooth muscle of rats.

Kasai

Tsutsumi

Taketani

et al. 1995

The Journal of Physiology

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1. We studied the effect of servo-controlled stretch of smooth muscle strips from rat uterus on tension and intracellular Ca2+ concentration ([Ca2+]i, using fura-2 as an indicator) at 300C. 2. When quiescent uterine muscle strips were stretched at a ramp time of 0 5 s by multiples of 5% of the resting muscle length (Lo) up to 40%, forty-two out of sixty muscle strips responded with a transient active contraction and a [Ca2+]i increase. The minimum excursion of stretch required for contraction was 26 3 + 7 5% of Lo (mean + S.D.). The peak response had an all-or-none property and was almost independent of the duration of stretch. 5. When a stretch of 15-35% of Lo was applied during the relaxation phase of 10 nM oxytocin-induced rhythmic contractions, the first contraction after the stretch occurred earlier than that expected from the control rhythm. However, the frequency of the subsequent rhythm returned to almost the control level even during continued application of stretch, although the half-width of rhythmic contractions was increased during stretch. 6. The present study demonstrates that stretch of uterine muscle induces a transient contraction due to Ca2+ influx, which is myogenic and dependent on the excursion and velocity of stretch. The all-or-none property of the stretch-induced contractions suggests initiation of Ca2+ spikes. Furthermore, stretch modulates the oxytocin-induced rhythmic contractions.

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Section: Discussionmentioning

confidence: 99%

Stretch‐induced enhancement of contractions in uterine smooth muscle of rats.

Kasai

Tsutsumi

Taketani

et al. 1995

The Journal of Physiology

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“…Gd 3+ ions inhibit Ca 2+ -selective stretch-activated channels at concentrations as low as 1 μM (30), but concentrations above 100 μM are required to inhibit the essentially nonselective bacterial channels MscS and MscL (31,51). In the later case, gadolinium ions have been shown to inhibit MS channels through interactions with negatively charged lipids in the membrane (51).…”

Section: Discussionmentioning

confidence: 99%

“…In summary, MSL10 forms a channel with a moderate preference for anions, passing ∼6 chloride ions for every sodium ion. Gadolinium ions are commonly used to inhibit K + , Ca 2+ , and metazoan MS channels (27) and have also been demonstrated to inhibit the activity of MS channels in plants (28)(29)(30) and bacteria (31). Inhibition of MSL10 activity was observed after excised inside-out patches were perfused in a bath containing 100 μM GdCl 3 (Fig.…”

Section: Msl10 Exhibits a Moderate Preference For Anionsmentioning

confidence: 99%

MscS-Like10 is a stretch-activated ion channel from Arabidopsis thaliana with a preference for anions

Maksaev

Haswell

2012

Proc. Natl. Acad. Sci. U.S.A.

132

156

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Like many other organisms, plants are capable of sensing and responding to mechanical stimuli such as touch, osmotic pressure, and gravity. One mechanism for the perception of force is the activation of mechanosensitive (or stretch-activated) ion channels, and a number of mechanosensitive channel activities have been described in plant membranes. Based on their homology to the bacterial mechanosensitive channel MscS, the 10 MscS-Like (MSL) proteins of Arabidopsis thaliana have been hypothesized to form mechanosensitive channels in plant cell and organelle membranes. However, definitive proof that MSLs form mechanosensitive channels has been lacking. Here we used single-channel patch clamp electrophysiology to show that MSL10 is capable of providing a MS channel activity when heterologously expressed in Xenopus laevis oocytes. This channel had a conductance of ∼100 pS, consistent with the hypothesis that it underlies an activity previously observed in the plasma membrane of plant root cells. We found that MSL10 formed a channel with a moderate preference for anions, which was modulated by strongly positive and negative membrane potentials, and was reversibly inhibited by gadolinium, a known inhibitor of mechanosensitive channels. MSL10 demonstrated asymmetric activation/ inactivation kinetics, with the channel closing at substantially lower tensions than channel opening. The electrophysiological characterization of MSL10 reported here provides insight into the evolution of structure and function of this important family of proteins.T he perception of mechanical stimuli like gravity, touch, or osmotic pressure is essential to normal plant growth and development and is further implicated in biotic and abiotic stress responses (1). One of the best-studied strategies for perceiving force involves membrane-embedded channels that are gated by tension, known as mechanosensitive (MS) channels (2). Numerous MS channel activities (>17 to date) have been described in the membranes of diverse tissues from a variety of plant species (summarized in ref. 3, also refs. 4 and 5). Many of these observed MS channel activities differ in their conductance, ion selectivity, and/or sensitivity to the direction of activation pressure, suggesting that multiple classes of mechanosensitive channels are present in plant cells.No mechanosensitive ion channel activity discovered in plant membranes has yet been definitively identified at the molecular level, but two families of proteins serve as strong candidates. The first is the Mid1-Complementing Activity (MCA) family, members of which are required for root response to touch in the model plant Arabidopsis thaliana, induce Ca 2+ uptake in rice and Arabidopsis cells (6, 7), and are associated with increased current in response to hypotonic stimulation of Xenopus oocytes (8). The second family of candidates for plant MS channels is the MscS-Like (MSL) family, first identified based on modest homology to the wellcharacterized bacterial MS channel MscS from Escherichia coli (3, 9). MscS is a lar...

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“…In bacteria, stretch-activated channels have been implicated: gadolinium inhibits both stretch-activated channel activity and loss of ions and metabolites in response to hypo-osmotic treatment (Berrier et al, 1992), whereas a two-component sensoreffector system activating Kt uptake has been implicated in hyperosmotic treatment (Walderhaug et al, 1992). Stretch-activated channels do occur in higher plants (Cosgrove and Hedrich, 1991), but in other organisms such as Neurospora crassa inhibition of stretchactivated channels by gadolinium does not increase the extent of hypo-osmotic-induced lysing of hyphae (Levina et al, 1995); therefore, they may not always play a role in sensing hypo-osmotic stress.…”

Section: I Sc U Sslonmentioning

confidence: 99%

Pressure Regulation of the Electrical Properties of Growing Arabidopsis thaliana L. Root Hairs

Lew

1996

Plant Physiology

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Gadolinium ion inhibits loss of metabolites induced by osmotic shock and large stretch‐activated channels in bacteria

Cited by 200 publications

References 31 publications

Stretch‐induced enhancement of contractions in uterine smooth muscle of rats.

Stretch‐induced enhancement of contractions in uterine smooth muscle of rats.

MscS-Like10 is a stretch-activated ion channel from Arabidopsis thaliana with a preference for anions

Pressure Regulation of the Electrical Properties of Growing Arabidopsis thaliana L. Root Hairs

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