Gaetbulibacter aestuarii sp. nov., isolated from shallow coastal seawater, and emended description of the genus Gaetbulibacter

Abstract

Gaetbulibacter aestuarii sp. nov., isolated from shallow coastal seawater, and emended description of the genus Gaetbulibacter A rod-shaped, yellow and strictly aerobic marine bacterium, designated KYW382 T , was isolated from seawater collected from the South Sea, Republic of Korea. Cells were Gram-negative and catalase-and oxidase-positive. The major fatty acids were iso-C 15 : 1 G, iso-C 15 : 0 , iso-C 17 : 0 3-OH, iso-C 15 : 0 3-OH and anteiso-C 15 : 0 . The DNA G+C content was 32.4 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain KYW382 T constituted an evolutionary lineage within the radiation enclosing the members of the genus Gaetbulibacter. The closest neighbour was Gaetbulibacter saemankumensis SMK-12 T (96.1 % 16S rRNA gene sequence similarity). A number of phenotypic characteristics distinguished strain KYW382 T from the described members of the genus Gaetbulibacter. On the basis of the data presented in this study, strain KYW382 T represents a novel species, for which the name Gaetbulibacter aestuarii sp. nov. is proposed. The type strain is KYW382 T (5KCTC 23303 T 5JCM 17455 T ). An emended description of the genus Gaetbulibacter is also given.

Gaetbulibacter lutimaris sp. nov., isolated from a tidal flat sediment

“…The major fatty acids (>10 % of the total) found in strain D1-y4 T were iso-C 15 : 0 (17.8 %), iso-C 15 : 1 G (15.9 %) and anteiso-C 15 : 0 (12.9 %). The fatty acid profile of strain D1-y4 T was similar to those of the type strains of the three Gaetbulibacter species, even though there were also differences in the proportions of some fatty acids, particularly iso-C 15 : 1 G (; Park et al , 2012). Polar lipids were extracted according to the procedures described by Minnikin et al (1984), and separated by two-dimensional TLC using chloroform/methanol/water (65 : 25 : 3.8, v/v) for the first dimension and chloroform/methanol/acetic acid/water (40 : 7.5 : 6 : 1.8, v/v) for the second dimension as described by Minnikin et al (1977).…”

“…The genus Gaetbulibacter , a member of the family Flavobacteriaceae (phylum Bacteroidetes ), was established by Jung et al (2005) with the description of Gaetbulibacter saemankumensis (type species), isolated from tidal flat sediment in the Yellow Sea in South Korea, as the sole recognized species. Subsequently, two further Gaetbulibacter species, Gaetbulibacter marinus and Gaetbulibacter aestuarii , were described from coastal seawaters in Korea (Yang & Cho, 2008; Park et al , 2012). In this study, a novel bacterial strain (designated D1-y4 T ), which was isolated from a tidal flat sediment in the South Sea in Korea, is described.…”

“… * Data for G. aestuarii KYW382 T taken from Park et al (2012). † Data for G. saemankumensis SMK-12 T taken from Jung et al (2005). ‡ Data for G . marinus KCTC 23046 T taken from Yang & Cho (2008). …”

Siansivirga zeaxanthinifaciens gen. nov., sp. nov., a novel zeaxanthin-producing member of the family Flavobacteriaceae isolated from coastal seawater of Taiwan

“… Data corresponding to the reference strains were obtained from the literature (2–4, Jung et al ., ; 5–6; Park et al ., ; except † which was obtained from Jung et al ., ). All remaining data given in the table were from this study. …”

“…Hydrolysis of casein, chitin, starch, xylan, CM‐cellulose (CMC), l ‐tyrosine, Tween 20 and Tween 80 was tested as given in Park et al . (), except that the culture plates were incubated at 30 °C for 5 days. DNase activity was analyzed using DNase test agar (Himedia) prepared using artificial seawater [ASW, 3.2% (w/v) synthetic sea salts (Sigma) in deionized water].…”