Siansivirga zeaxanthinifaciens gen. nov., sp. nov., a novel zeaxanthin-producing member of the family Flavobacteriaceae isolated from coastal seawater of Taiwan

Abstract

A strictly aerobic, Gram-negative, rod-shaped bacterium (strain CC-SAMT-1(T)) showing gliding motility was isolated from coastal seawater of China Sea, Taiwan. Strain CC-SAMT-1(T) synthesizes all-trans-zeaxanthin (6.5 ± 0.5 mg g(-1) dry biomass) as a predominant xanthophyll carotenoid. As determined by 16S rRNA gene analysis, strain CC-SAMT-1(T) shared very high sequence similarity to the members of the genera Mariniflexile (96.1-95.3%) and Gaetbulibacter (96.0-95.9%); however, it formed a distinct phyletic lineage distantly associated with Mariniflexile species. Polar lipid profile constitutes phosphatidylethanolamine, four unidentified aminolipids, four unidentified lipids, and an unidentified glycolipid. Strain CC-SAMT-1(T) contains excessive unidentified aminolipid lipid (AL2-4) and glycolipid contents, and therefore clearly distinct from Mariniflexile species. Major fatty acids (> 5% of total fatty acids) were iso-C(15:0) (14.8%), iso-C(17:0) 3-OH (11.8%), iso-C(15:1) G (10.6%), anteiso-C(15:0) (9.7%), C(16:0) (8.1%), iso-C(16:0) 3-OH (7.9%), iso-C(15:0) 3-OH (7.5%), and summed feature 3 (containing C(16:1) ω6c and/or C(16:1) ω7c) (7.5%). Menaquinone-6 (MK-6) was major respiratory quinone. DNA G+C content was 33.7 mol%. Based on polyphasic taxonomy, strain CC-SAMT-1(T) represents a novel genus and species in the family Flavobacteriaceae for which the name Siansivirga zeaxanthinifaciens gen. nov., sp. nov. is proposed. The type strain is CC-SAMT-1(T) (= BCRC 80315(T) = JCM 17682(T)).

Sphingomicrobium astaxanthinifaciens sp. nov., an astaxanthin-producing glycolipid-rich bacterium isolated from surface seawater and emended description of the genus Sphingomicrobium

“…Carotenoids were extracted and analysed according to Hameed et al (2012) with the following modifications: authentic all-trans-astaxanthin was used as a standard for qualitative and quantitative determination of astaxanthin; detection wavelength was set to 476 nm during reversedphase HPLC. Respiratory quinones of strains CC-AMO-30B…”

Hanstruepera neustonica gen. nov., sp. nov., a zeaxanthin-producing member of the family Flavobacteriaceae isolated from estuarine water, and emendation of Sediminibacter furfurosus Khan et al. 2007 emend. Kwon et al. 2014, Mangrovimonas yunxiaonensis Li et al. 2013, Antarcticimonas flava Yang et al. 2009 and Hoppeia youngheungensis Kwon et al. 2014

“…The number of representatives of the family Flavobacteriaceae is constantly increasing through the continuous description of new taxa. We have characterized species such as Lutaonella thermophila (Arun et al , 2009a), Muricauda lutaonensis (Arun et al , 2009b), Siansivirga zeaxanthinifaciens (Hameed et al , 2012), Kordia aquimaris (Hameed et al , 2013), Gramella planctonica (Shahina et al , 2014), Aquibacter zeaxanthinifaciens (Hameed et al , 2014a), Robertkochia marina (Hameed et al , 2014b) and Gramella oceani (Hameed et al , 2014c) from various marine locations in Taiwan. Here, we report on the taxonomic characterization of a novel zeaxanthin-producing flavobacterial strain, designated CC-PY-50 T , which was isolated from Formosan estuarine water.…”

“…The presence of flexirubin-type pigments was investigated as described by Reichenbach (1992) and Bernardet et al (2002). Carotenoids were extracted and analysed according to earlier descriptions (Hameed et al , 2012). The presence of the proteorhodopsin gene was tested using three pairs of degenerate primers (PR-Flavo-F and R, PR-Flavo-2F and 2R, and SA4-10 PRno3F and 3R) according to the descriptions of Yoshizawa et al (2012).…”

Haloflavibacter putidus gen. nov., sp. nov., isolated from coastal seawater

“…The temperature range for growth was determined on MA at 4, 10, 15, 20, 25, 28, 30, 33, 37 and 40 ℃. to previously published methods [37]. Susceptibility to antibiotics was investigated on MA using the disc diffusion method as described previously [38], and according to procedures outlined by the Clinical and Laboratory Standards Institute [39].…”