Sphingomicrobium astaxanthinifaciens sp. nov., an astaxanthin-producing glycolipid-rich bacterium isolated from surface seawater and emended description of the genus Sphingomicrobium

Abstract

Sphingomicrobium astaxanthinifaciens sp. nov., an astaxanthin-producing glycolipid-rich bacterium isolated from surface seawater and emended description of the genus Sphingomicrobium Biotechnology Center, National Chung Hsing University, Taichung 402, TaiwanA Gram-stain-negative, rod-shaped, strictly aerobic, flagellated and non-spore-forming marine bacterium designated strain CC-AMO-30B T was isolated from coastal surface seawater, Taiwan.Strain CC-AMO-30B T synthesized astaxanthin [40 mg (g dry weight)"1

Hanstruepera neustonica gen. nov., sp. nov., a zeaxanthin-producing member of the family Flavobacteriaceae isolated from estuarine water, and emendation of Sediminibacter furfurosus Khan et al. 2007 emend. Kwon et al. 2014, Mangrovimonas yunxiaonensis Li et al. 2013, Antarcticimonas flava Yang et al. 2009 and Hoppeia youngheungensis Kwon et al. 2014

“…The genomic DNA of strain CC-PY-50 T was isolated using an UltraClean Microbial Genomic DNA Isolation kit (MO BIO) by following the manufacturer’s instructions. The partial 16S rRNA gene was amplified via PCR according to Shahina et al (2013). Gene sequencing was performed by using a BigDye terminator kit (Heiner et al , 1998) and an automatic DNA sequencer (ABI PRISM 310, Applied Biosystems) (Watts & MacBeath, 2001).…”

“…The respiratory quinones of strain CC-PY-50 T were extracted according to the protocol of Minnikin et al (1984) and analysed by reverse-phase HPLC according to the method of Collins (1985) with minor modifications (Shahina et al , 2013). The polar lipids of strain CC-PY-50 T and reference strains were extracted and analysed by two-dimensional TLC (Embley & Wait, 1994).…”

“…For determination of the G+C content, the DNA of strain CC-PY-50 T was subjected to thermal denaturation followed by enzymic digestion into nucleosides as described by Mesbah et al (1989). The resultant nucleoside mixture was separated and quantified by reverse-phase HPLC with minor modifications as given by Shahina et al (2013).…”

Sphingomicrobium aestuariivivum sp. nov., isolated from a tidal flat

“…The genus Sphingomicrobium , a member of the class Alphaproteobacteria , was proposed by Kämpfer et al (2012) with the description of a single recognized species, Sphingomicrobium lutaoense , isolated from water of a coastal hot spring of Green Island, Taiwan. Subsequently, three further species of the genus Sphingomicrobium have been described, Sphingomicrobium astaxanthinifaciens (Shahina et al , 2013a) and Sphingomicrobium flavum and Sphingomicrobium marinum (Shahina et al , 2013b). Members of the genus Sphingomicrobium have been isolated from marine environments including water from a coastal hot spring and surface seawater (Kämpfer et al 2012; Shahina et al , 2013a, b).…”

“…Subsequently, three further species of the genus Sphingomicrobium have been described, Sphingomicrobium astaxanthinifaciens (Shahina et al , 2013a) and Sphingomicrobium flavum and Sphingomicrobium marinum (Shahina et al , 2013b). Members of the genus Sphingomicrobium have been isolated from marine environments including water from a coastal hot spring and surface seawater (Kämpfer et al 2012; Shahina et al , 2013a, b). In this study, we describe a novel bacterial strain, designated AH-M8 T , which was isolated from a tidal flat sediment of Aphae Island, located in the south-western sea of the Korean peninsula.…”