Galactan sulfate of Grateloupia indica: Isolation, structural features and antiviral activity

Chattopadhyay, Kausik; Mateu, Cecilia Gabriela; Mandal, Pinaki; Pujol, Carlos A.; Damonte, Elsa B.; Ray, Bimalendu

doi:10.1016/j.phytochem.2007.02.008

Cited by 78 publications

(53 citation statements)

References 40 publications

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“…The approximately 10-fold higher yield of total carbohydrates measured by the Dubois method suggests that both reducing and neutral sugars were released by the enzymatic treatment. This result is in agreement with an earlier report, which described water-extracted carbohydrates from G. indica (Chattopadhyay et al 2007). In addition, the present data show that the amount of total carbohydrates was more variable than the amount of reducing sugars.…”

Section: Discussionsupporting

confidence: 94%

An evaluation of methods for quantifying the enzymatic degradation of red seaweed Grateloupia turuturu

et al. 2008

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For better exploitation of the red seaweed Grateloupia, enzymatic digestion of the thallus may be a way to increase access to metabolites of industrial interest. With this aim, we have tried to find a method to quantify the efficiency of enzymatic digestion. Vegetative algal material was treated with polysaccharidases (Onozuka R-10 cellulase, agarase, and Ultraflo L mixture). The proportion of degraded surface area was determined by microscopic measurement of the residue surface using imaging software and compared with the analysis of carbohydrates and Rphycoerythrin released in the incubation solution. Both the reducing carbohydrate concentration and percentage of degraded surface area appeared the most reliable methods to study enzymatic efficiency. The amount of solubilized total carbohydrates, and particularly that of R-phycoerythrin, showed non-specific variations, so no conclusions could be drawn. The application of this procedure to the screening of the efficient digestion of Grateloupia material demonstrated that cell walls were only partially digested by polysaccharidase enzymes alone. The Ultraflo L mixture and Onozuka R-10 cellulase produced a greater degradation of Grateloupia tissues and a higher release of reducing carbohydrates, whereas agarase did not display any specific action. Thus, the proposed procedure based on the quantification of residue surface area seems to be an accurate method to analyze enzymatic digestion. Other tests using different concentrations and combinations of enzymes are now required.

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Section: Discussionsupporting

confidence: 94%

An evaluation of methods for quantifying the enzymatic degradation of red seaweed Grateloupia turuturu

et al. 2008

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“…However, as these compounds are not always efficacious or well tolerated and drug-resistant strains are rapidly emerging, the demand for antiviral drugs with novel mode of action is great. In recent years, screening assays of the antiviral activity of extracts from marine algae have led to the identification of a number of sulfated polysaccharides with potent in vitro inhibitory effects against various animal virus including herpes simplex virus (HSV) types 1 and 2 [3][4][5][6][7][8]. These polysaccharides include predominantly fucoidans, sulfated galactans, sulfated mannans and sulfated heteroglycans.…”

Section: Introductionmentioning

confidence: 99%

Polysaccharides from Gracilaria corticata: Sulfation, chemical characterization and anti-HSV activities

Chattopadhyay

Ghosh

Pujol

et al. 2008

International Journal of Biological Macromolecules

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“…For the determination of sugar composition, the monosaccharide residues released by acid hydrolysis were converted into their alditol acetate [12] and analysed by gas-liquid chromatography (GLC; Shimadzu GC-17A; Shimadzu, Kyoto, Japan). Monosaccharides were identified by thin-layer chromatography and GLC-mass spectrometry (GLC-MS; Shimadzu QP 5050 A; Shimadzu) as described previously [13]. Alternatively, tetramethyl silyl (TMS) derivatives of methyl glycosides were analysed by GLC [14].…”

Section: Chemical Analysesmentioning

confidence: 99%

Sulfated Xylomannans from the Red Seaweed Sebdenia Polydactyla: Structural Features, Chemical Modification and Antiviral Activity

Ghosh

Pujol

Damonte

et al. 2009

Antivir Chem Chemother

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Background: Many viruses display affinity for cell surface heparan sulfate proteoglycans with biological relevance in virus entry. This raises the possibility of the application of sulfated polysaccharides in antiviral therapy. Methods: In this study, we analysed polysaccharide fractions isolated from Sebdenia polydactyla. Results: The purified xylomannan sulfate and its further sulfated derivatives showed strong activity against herpes simplex virus type-1 (HSV-1). Their 50% inhibitory concentration values were in the range 0.35-2.8 µg/ ml and they lacked cytotoxicity at concentrations up to 1,000 µg/ml. The major polysaccharide, which had 0.6 sulfate groups per monomer unit and an apparent molecular mass of 150 kDa, contained a backbone of α-(1→3)-linked d-mannopyranosyl residues substituted at position 6 with a single stub of β-d-xylopyranosyl residues. Conclusions: The degree of sulfation seemed to play an important role because desulfation and/or further sulfation of the isolated macromolecules largely influenced their in vitro anti-HSV-1 activity.

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Galactan sulfate of Grateloupia indica: Isolation, structural features and antiviral activity

Cited by 78 publications

References 40 publications

An evaluation of methods for quantifying the enzymatic degradation of red seaweed Grateloupia turuturu

An evaluation of methods for quantifying the enzymatic degradation of red seaweed Grateloupia turuturu

Polysaccharides from Gracilaria corticata: Sulfation, chemical characterization and anti-HSV activities

Sulfated Xylomannans from the Red Seaweed Sebdenia Polydactyla: Structural Features, Chemical Modification and Antiviral Activity

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