Galactomannan of Delonix regia seeds modulates cytokine expression and oxidative stress eliciting anti-inflammatory and healing effects in mice cutaneous wound

Abstract

Objective and designTo investigate the healing mechanism of Delonix regia galactomannan (GM-DR) in a mice model of excisional cutaneous wound. Materials and subjectsFemale Swiss mice were used in all treatments.Treatment GM-DR (% 0.01-1) was topically applied to the wounds during 14 days. MethodsThe wound healing effect of GM-DR was evaluated by the following parameters: wound closure, clinical signs (hyperemia, edema, exsudate, nociception), oxidative stress markers (malondialdehyde -MDA, reduced glutathione -GSH), histopathological and histomorphometric analysis (collagenesis, blood vessels, polymorphonuclear, mononuclear, broblast/myo broblast cells) and immunohistochemical (in ammatory growth factor mediators). ResultsGM-DR reduced wound area (7 -14 th day) and hypernociception (6 h -5 th day), leukocyte in ltration (2 -7 th day), expression and levels of IL-1β (2 th day), IL-6 (2 th day), MDA (44% -2 th day), and increased broblast/myo broblast, granulation tissue, collagen deposition, GSH (25 -50%, 2-5 th day), Transforming Growth Factor Beta (TGF-β) expression (7-10 th day) and Smooth Muscle Alpha Actin (a-SMA) (7-14 th day). ConclusionsGM-DR accelerates the mice healing process acting both in the in ammatory and proliferative phases.

Encapsulation of Polyphenolic Compounds Based on Hemicelluloses to Enhance Treatment of Inflammatory Bowel Diseases and Colorectal Cancer

“…For instance, galactomannans extracted from Chinese Sesbania cannabina may increase the level of superoxide dismutase (SOD) and limit the production of intracellular ROS [ 123 , 124 ]. Other studies indicate that galactomannans deriving from Delonix regia and locust bean gum may have pro-healing properties [ 125 , 126 ]; this would be desirable for intestinal mucosal healing, regeneration, and improving intestinal barrier integrity in IBD. The galactomannan of Delonix regia seeds is able to limit inflammatory cell infiltration and increase fibroplasia and collagenesis by enhancing transforming growth factor beta (TGF-β) and alpha smooth muscle actin (α-SMA) protein expression in wounds.…”

Potential metabolites of Arecaceae family for the natural anti-osteoarthritis medicine: A review

“…One of the polysaccharides found in the Arecaceae family is galactomannan. Research suggests galactomannan from Delonix regia has anti-inflammatory and antinociceptive effects [ 133 ]. Galactomannan also reduces inflammatory symptoms by decreasing neutrophil infiltration, leukocyte migration and adhesion, TNFα production, and thiobarbituric acid reactive species (TBARS) [ 134 ].…”

“…For example, galactomannan polysaccharide compounds found in sugar palm, palmyra palm, and nipa palm fruits can be developed in OA treatments. Galactomannan has anti-inflammatory and chondroprotective effects that can reduce cartilage degradation [ 105 , 143 ]. According to the research, protein-free galactomannan provides a better anti-OA result.…”

Anti‐inflammatory and healing effect of the polysaccharidic extract of Opuntia ficus‐indica cladodes in cutaneous excisional wounds in rats

“…In this perspective, plant polysaccharides have emerged as an alternative due to their proven pharmacological activities: anti-inflammatory and antinociceptive, 5,6 antioxidant 6 and healing. [7][8][9] Opuntia ficus-indica (Cactaceae) is a species of cactus endemic to the northeast region of Brazil, and its use is documented in folk medicine to treat pelvic inflammatory processes and rheumatism 10 Its polysaccharides have anti-inflammatory, [11][12][13] wound healing, 14,15 gastroprotective and antioxidant effects. 16,17 Cactaceae cladodes are particularly rich in carbohydrate-containing polymers with several sugar residues such as arabinose, galactose, rhamnose, xylose and galacturonic acid.…”

“…The diversity of flora around the world stimulates the search for alternatives to compounds already used therapeutically. In this perspective, plant polysaccharides have emerged as an alternative due to their proven pharmacological activities: anti‐inflammatory and antinociceptive, 5,6 antioxidant 6 and healing 7‐9 . Opuntia ficus‐indica (Cactaceae) is a species of cactus endemic to the northeast region of Brazil, and its use is documented in folk medicine to treat pelvic inflammatory processes and rheumatism 10 Its polysaccharides have anti‐inflammatory, 11‐13 wound healing, 14,15 gastroprotective and antioxidant effects 16,17 …”