Galactooligosaccharides and 2′-fucosyllactose can directly suppress growth of specific pathogenic microbes and affect phagocytosis of neutrophils

Mortaz, Esmaeil; Nomani, Masoumeh; Adcock, Ian M.; Folkerts, Gert; Garssen, Johan

doi:10.1016/j.nut.2022.111601

Cited by 9 publications

(9 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…42 Furthermore, at lower concentrations, FL exhibited similar antibacterial properties to GOS, including inhibiting the growth of Staphylococcus aureus and Pseudomonas aeruginosa, and enhancing the ability of neutrophils to phagocytose bacteria. 43 In the same dose, FL was more efficacious than GOS in ameliorating DSS-induced colitis by enhancing the ability of the microbiota to metabolize secondary bile acids with further inhibition of the G proteincoupled bile acid receptor 1-mediated NF-κB pathway. 41 However, comparative studies on the preventive and ameliorative effects of GOS and FL on HFrD-exacerbated colitis in mice are lacking.…”

Section: ■ Introductionmentioning

confidence: 92%

“…In an in vitro cell model, Cheng et al found that GOS, but not FL, promoted Muc-2 synthesis and Muc-2 protein secretion by regulating goblet cell function . Furthermore, at lower concentrations, FL exhibited similar antibacterial properties to GOS, including inhibiting the growth of Staphylococcus aureus and Pseudomonas aeruginosa , and enhancing the ability of neutrophils to phagocytose bacteria . In the same dose, FL was more efficacious than GOS in ameliorating DSS-induced colitis by enhancing the ability of the microbiota to metabolize secondary bile acids with further inhibition of the G protein-coupled bile acid receptor 1-mediated NF-κB pathway .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Galactooligosaccharide or 2′-Fucosyllactose Modulates Gut Microbiota and Inhibits LPS/TLR4/NF-κB Signaling Pathway to Prevent DSS-Induced Colitis Aggravated by a High-Fructose Diet in Mice

Chen

Wang

Nie

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

A high-fructose diet (HFrD) has been reported to exacerbate dextran sulfate sodium (DSS)-induced colitis. 2′-Fucosyllactose (FL) and galactooligosaccharide (GOS) have been shown, respectively, to have preventive and ameliorative effects on colitis, while limited research has explored whether GOS and FL may be equally protective or preventive in mice with HFrD. Here, we evaluated the protective effects of FL and GOS on colitis exacerbated by feeding HFrD and explored the underlying mechanisms. DSS-induced colitis was studied in four randomized C57BL/6J male mice (n = 8 mice/group). Among them, three groups were fed with HFrD, and two received either GOS or FL treatment, respectively. Gut microbial composition was analyzed by 16S rDNA gene sequencing. Intestinal barrier integrity and inflammatory pathway expression were measured using qPCR, immunofluorescence, and Western blot methods. Compared to the HFrD group, GOS or FL treatment increased the α-diversity of the gut microbiota, reduced the relative abundance of Akkermansia, and increased the content of short-chain fatty acids (SCFAs), respectively. Compared with the HFrD group, GOS or FL treatment improved the loss of goblet cells and the reduction of tight junction protein expression, thereby improving intestinal barrier integrity. Also, GOS or FL inhibited the LPS/TLR4/NF-κB signaling pathway and oxidative stress to suppress the inflammatory cascade compared with the HFrD group. These findings suggest that GOS or FL intake can alleviate HFrD-exacerbated colitis, with no significant difference observed between GOS and FL treatments.

show abstract

Section: ■ Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Galactooligosaccharide or 2′-Fucosyllactose Modulates Gut Microbiota and Inhibits LPS/TLR4/NF-κB Signaling Pathway to Prevent DSS-Induced Colitis Aggravated by a High-Fructose Diet in Mice

Chen

Wang

Nie

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Then we designed and created bifunctional and programmable genetic circuits by combining CRISPRi-based NOT gates as repressor system. Finally, as a proof-of-concept, we used the temperature-responsive genetic circuits for multi-modular ordered control of the production of 2′-fucosyllactose (2′-FL), which is one of the most abundant human milk oligosaccharides (HMOs) with beneficial effects on the microbiota of neonates by preventing infection from pathogenic bacteria ( 19 , 20 ). This work provides a novel and effective toolkit for dynamic regulation in B. subtilis and the framework here developed for the design and optimization of temperature-responsive genetic circuits may be useful for engineering the other microbes.…”

Section: Introductionmentioning

confidence: 99%

A pathway independent multi-modular ordered control system based on thermosensors and CRISPRi improves bioproduction in Bacillus subtilis

Jin

et al. 2022

Nucleic Acids Research

View full text Add to dashboard Cite

Dynamic regulation is an effective strategy for control of gene expression in microbial cell factories. In some pathway contexts, several metabolic modules must be controlled in a time dependent or ordered manner to maximize production, while the creation of genetic circuits with ordered regulation capacity still remains a great challenge. In this work, we develop a pathway independent and programmable system that enables multi-modular ordered control of metabolism in Bacillus subtilis. First, a series of thermosensors were created and engineered to expand their thresholds. Then we designed single-input-multi-output circuits for ordered control based on the use of thermosensors with different transition points. Meanwhile, a repression circuit was constructed by combining CRISPRi-based NOT gates. As a proof-of-concept, these genetic circuits were applied for multi-modular ordered control of 2′-fucosyllactose (2′-FL) biosynthesis, resulting in a production of 1839.7 mg/l in shake flask, which is 5.16-times that of the parental strain. In a 5-l bioreactor, the 2′-FL titer reached 28.2 g/l with down-regulation of autolysis. Taken together, this work provides programmable and versatile thermosensitive genetic toolkits for dynamic regulation in B. subtilis and a multi-modular ordered control framework that can be used to improve metabolic modules in other chassis cells and for other compounds.

show abstract

“…Os Galactooligossacarídeos (GOS), os fruto-oligossacarídeos (FOS) e a inulina, são atualmente considerados os prebióticos mais aceites e estudados (6)(7)(8)(9)(10)(11). São denominados "prebióticos", uma vez que são hidratos de carbono não digeríveis que conferem benefícios à saúde do hospedeiro ao estimularem seletivamente o crescimento e/ou ou atividade de membros específicos da microbiota intestinal, dificultando a proliferação de bactérias patogénicas (2,4,6,(8)(9)(10)(11)(12)(13)(14)(15)(16).…”

Section: Introductionunclassified

“…Quanto à sua estrutura, os prebióticos são oligossacarídeos funcionais de cadeia curta, compostos por polímeros de galactose com um monómero terminal de glicose. Podem ser ingeridos naturalmente pelo consumo de alimentos que os contém na sua composição, principalmente hortofrutícolas, pelo consumo de alimentos enriquecidos nos mesmos ou por meio de suplementação, por exemplo, em laticínios, fórmulas infantis e pão (14,17,18). Os β-GOS, são GOS que possuem glicose terminal β-ligada e são produzidos a partir da lactose usando β-galactosidases como substrato para catalisar as reações de transgalactosilação (8,9,17).…”

Section: Introductionunclassified

Galactooligossacarídeos: existem efeitos na microbiota humana?

Costa,

Brito,

Pereira

et al. 2022

Acta Port Nutr

View full text Add to dashboard Cite

Galactooligossacarídeos são denominados “prebióticos”, uma vez que são hidratos de carbono não digeríveis que conferem diversos benefícios para a saúde do hospedeiro, entre eles, a modulação da microbiota intestinal. Podem ser ingeridos naturalmente pelo consumo de alimentos que os contém na sua composição, pelo consumo de alimentos enriquecidos nos mesmos ou por meio de suplementação. Foi efetuada uma revisão de literatura, com pesquisa nas bases de dados Pubmed, Google Académico e Science Direct, com as palavras-chave “galacto-oligosaccharides’’ AND “effects’’ AND “microbiota’’ AND ‘’health’’, entre 2017 e 2022, com o objetivo de compreender de que forma a ingestão de Galactooligossacarídeos pode influenciar a microbiota intestinal humana. Os resultados da maioria dos artigos evidenciaram correlação positiva entre o consumo de Galactooligossacarídeos, quer proveniente da alimentação, ou suplementação, com a modulação benéfica da microbiota intestinal, aumentando o número de bactérias benéficas e reduzindo as prejudiciais, em qualquer faixa etária. Assim, a adoção de uma dieta enriquecida em Galactooligossacarídeos poderá ser uma estratégia eficaz em situações de disbiose, atuando de forma preventiva e benéfica na melhoria da microbiota intestinal.

show abstract

Galactooligosaccharides and 2′-fucosyllactose can directly suppress growth of specific pathogenic microbes and affect phagocytosis of neutrophils

Cited by 9 publications

References 40 publications

Galactooligosaccharide or 2′-Fucosyllactose Modulates Gut Microbiota and Inhibits LPS/TLR4/NF-κB Signaling Pathway to Prevent DSS-Induced Colitis Aggravated by a High-Fructose Diet in Mice

Galactooligosaccharide or 2′-Fucosyllactose Modulates Gut Microbiota and Inhibits LPS/TLR4/NF-κB Signaling Pathway to Prevent DSS-Induced Colitis Aggravated by a High-Fructose Diet in Mice

A pathway independent multi-modular ordered control system based on thermosensors and CRISPRi improves bioproduction in Bacillus subtilis

Galactooligossacarídeos: existem efeitos na microbiota humana?

Contact Info

Product

Resources

About