2004
DOI: 10.1109/jproc.2003.820551
|View full text |Cite
|
Sign up to set email alerts
|

Galactosidase Assays of Single-Cell Lysates on a Microchip: A Complementary Method for Enzymatic Analysis of Single Cells

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
25
0

Year Published

2007
2007
2016
2016

Publication Types

Select...
6
1
1

Relationship

0
8

Authors

Journals

citations
Cited by 43 publications
(25 citation statements)
references
References 41 publications
0
25
0
Order By: Relevance
“…For microfluidic chips, different mixing methods must be used. Ocvirk et al (2004) used a Y-shaped microfluidic chip to mix a cell stream containing physiological buffer with a lysis buffer stream containing detergent and the substrate of the enzyme b-galactosidase. Because they were ultimately interested in enzyme activity within the cell, they chose the slower lysing 0.1% Triton X-100 (30 s) over the faster lysing 0.5% SDS (less than 2 s), in order to retain enzyme activity.…”
Section: Detergent Lysismentioning
confidence: 99%
“…For microfluidic chips, different mixing methods must be used. Ocvirk et al (2004) used a Y-shaped microfluidic chip to mix a cell stream containing physiological buffer with a lysis buffer stream containing detergent and the substrate of the enzyme b-galactosidase. Because they were ultimately interested in enzyme activity within the cell, they chose the slower lysing 0.1% Triton X-100 (30 s) over the faster lysing 0.5% SDS (less than 2 s), in order to retain enzyme activity.…”
Section: Detergent Lysismentioning
confidence: 99%
“…Whenever cell lysis is required, from the different existing alternatives (Brown and Audet, 2008), reagent-less mechanical approaches (Di Carlo et al, 2003) and detergent lysis (Ocvirk et al, 2004;Huang et al, 2007) are the most directly exploitable for ADDCA.…”
Section: Sample Preparationmentioning
confidence: 99%
“…Ocvirk et al [137] designed a microfluidic glass chip to determine the presence of the same enzyme. Wang et al [135] demonstrated a microfluidic device which carried out chemical analysis using electrical lysis.…”
Section: Proteinsmentioning
confidence: 99%