Gallium Diffusion in Human Root Dentin: Quantitative Measurements by Pulsed Nd:YAG Laser Ablation Combined with an Inductively Coupled Plasma Mass Spectrometer

Ghazi, A. Mohamad; Shuttleworth, S.; Angulo, S J; Pashley, David H.

doi:10.1089/10445470050144029

Cited by 22 publications

(21 citation statements)

References 20 publications

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“…This was consistent with previous reports, where gallium nitrate was placed in human root canals to assess whether it would diffuse across root dentine and reach concentrations high enough to inhibit osteoclasts [Ghazi et al, 2000]. The report showed that the gallium concentration was highest adjacent to the root canal space and fell as more peripheral sites were sampled, but then rose slightly at the external boundary of the root which is covered with a thin layer of a tubular cementum [Ghazi et al, 2000].…”

Section: Activity Of Gallium On S Mutans and Dental Tissuessupporting

confidence: 90%

“…The report showed that the gallium concentration was highest adjacent to the root canal space and fell as more peripheral sites were sampled, but then rose slightly at the external boundary of the root which is covered with a thin layer of a tubular cementum [Ghazi et al, 2000]. It was reported that in vivo administration of Ga 3+ interferes with osseous tissue HA generation, resulting in larger and more perfect (lowered CO 3 :PO 4 ratios) crystalline structures [Bockman et al, 1986].…”

Section: Activity Of Gallium On S Mutans and Dental Tissuesmentioning

confidence: 99%

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Effect of Gallium on Growth of Streptococcus mutans NCTC 10449 and Dental Tissues

Valappil

Owens²,

Miles³

et al. 2013

Caries Res

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Gallium-doped phosphate-based glasses (Ga-PBG) were assessed for their impact on Streptococcus mutans and dental mineralisation, firstly by disc diffusion assays followed by biofilms grown on nitrocellulose filter membrane (NFM) and constant-depth film fermentor (CDFF). Short-time exposure (10 min) effects of Ga-PBG on S. mutans biofilm were compared with that of 0.2% chlorhexidine. The effects of Ga-PBG on bovine enamel (which was investigated under pH-cycling condition) and dentine were analysed using transverse microradiography (TMR), profilometry and inductively coupled plasma optical-emission spectrometry (ICP-OES). The disc diffusion assays showed inhibition zones of 24.5 ± 0.5 mm for Ga-PBG compared with controls (C-PBG). Ga-PBG showed statistically significant growth inhibition of S. mutans biofilms on NFM (p = 0.001) and CDFF (p < 0.046) compared with hydroxyapatite (HA) and C-PBG. The CDFF assay revealed a maximum of 2.11 log colony-forming unit (CFU) reduction at 48 h, but short-time exposure effects were comparable with that of 0.2% chlorhexidine only on older biofilms (maximum of 0.59 vs. 0.69 log CFU reduction at 120 h). TMR analyses of the enamel revealed non-significant mineral loss (p = 0.37) only in the case of Ga-PBG samples compared with controls including sodium fluoride. ICP-OES analyses indicated transient gallium adsorption into dentine by calcium displacement. The results confirmed that gallium inhibited S. mutans growth and appears to have the potential to protect the enamel surface under conditions representative of the oral environment. Further work is needed to establish whether it has an application in daily oral hygiene procedures to prevent or reduce caries.

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Section: Activity Of Gallium On S Mutans and Dental Tissuessupporting

confidence: 90%

Section: Activity Of Gallium On S Mutans and Dental Tissuesmentioning

confidence: 99%

Effect of Gallium on Growth of Streptococcus mutans NCTC 10449 and Dental Tissues

Valappil

Owens²,

Miles³

et al. 2013

Caries Res

View full text Add to dashboard Cite

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“…Investigations on Hg migration [157] and Ga diffusion across the human teeth using LA-ICP-MS were also reported [158,159].…”

Section: Teeth Analysismentioning

confidence: 99%

Laser ablation in analytical chemistry—a review

Russo

2002

Talanta

524

335

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Laser ablation is becoming a dominant technology for direct solid sampling in analytical chemistry. Laser ablation refers to the process in which an intense burst of energy delivered by a short laser pulse is used to sample (remove a portion of) a material. The advantages of laser ablation chemical analysis include direct characterization of solids, no chemical procedures for dissolution, reduced risk of contamination or sample loss, analysis of very small samples not separable for solution analysis, and determination of spatial distributions of elemental composition. This review describes recent research to understand and utilize laser ablation for direct solid sampling, with emphasis on sample introduction to an inductively coupled plasma (ICP). Current research related to contemporary experimental systems, calibration and optimization, and fractionation is discussed, with a summary of applications in several areas.

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“…in the past, ion presence was determined through the acidulation of all of the tissue to be examined. however, it is impossible to obtain detailed results with these techniques (14). lA-icP-MS is a technique determining the ion release in the solid materials reliably and in a short time (31).…”

Section: Resultsmentioning

confidence: 99%