GALNT7, a target of miR-494, participates in the oncogenesis of nasopharyngeal carcinoma

Nie, Guohui; Luo, Liang; Duan, Hongfang; Li, Xiaoqing; Yin, Meijun; Zhao, Li; Zhang, Wei

doi:10.1007/s13277-015-4281-6

Cited by 17 publications

(12 citation statements)

References 29 publications

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“…The expression level of GALNT7 was dramatically decreased in SiHa and Caski cells after overexpression of miR-30e. It has been reported that GALNT7 plays a role in carcinogenesis and metastasis in many common cancers [30] , [37] . Meanwhile, GALNT7 is found to be upregulated in cancers such as laryngeal carcinoma and cervical cancer [36] , [38] .…”

Section: Discussionmentioning

confidence: 99%

“…The O-glycosylation of these glycoproteins is considered to play a key role in determining tumor properties in many cases of cancer, such as cervical cancer, hepatocellular carcinoma, and esophageal squamous cell cancer [17] , [28] , [29] . It has been reported that GALNT7 was targeted by some of miRNAs, such as miR-214, miR-494, and miR-17, in different cancers [17] , [28] , [30] . However, the expression and the regulation of GALNT7 in cervical cancer remain obscure.…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA-30e Functions as a Tumor Suppressor in Cervical Carcinoma Cells through Targeting GALNT7

Liu

et al. 2017

Translational Oncology

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Cervical cancer is the third most common cancer in women worldwide. However, the underlying mechanism of occurrence and development of cervical cancer is obscure. In this study, we observed that miR-30e was downregulated in clinical cervical cancer tissues and cervical cancer cells. Next, overexpression of miR-30e reduced the cervical cancer cell growth through MTT, colony formation, EdU, and Transwell assay in SiHa and Caski cells. Subsequently, UDP-N-acetyl-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase 7 (GALNT7) was identified as a potential miR-30e target by bioinformatics analysis. Moreover, we showed that miR-30e was able to bind to the 3′UTR of GALNT7 by luciferase reporter assay. In addition, the mRNA and protein levels of GALNT7 in cervical cancer cells were downregulated by miR-30e. And we validated that downregulation of GALNT7 repressed the proliferation of SiHa and Caski cells by MTT, colony formation, and Transwell assay. We identified that the restoration of GALNT7 expression was able to counteract the effect of miR-30e on cell proliferation of cervical cancer cells. Furthermore, we found that the expression levels of GALNT7 were frequently upregulated and negatively correlative to those of miR-30e in cervical cancer tissues. In addition, we validated that restoration of GALNT7 rescued the miR-30e–suppressed growth of cervical cancer xenografts in vivo. In conclusion, the current results suggest that miR-30e may function as tumor suppressors in cervical cancer through downregulation of GALNT7. Both miR-30e and its novel target, GALNT7, may play an important role in the process of cervical cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MicroRNA-30e Functions as a Tumor Suppressor in Cervical Carcinoma Cells through Targeting GALNT7

Liu

et al. 2017

Translational Oncology

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“…Initiation of O-glycosylation Identified as oncogene in several types of cancer and controlled by micro-RNAs (Gaziel-Sovran et al , Peng et al 2012, Li et al 2014b, Nie et al 2015, Lu et al 2016 Upregulated in malignant PCa as part of a glycosylation gene signature (Barfeld et al 2014a). Androgen regulated and linked to prostate cancer cell viability ) GCNT1…”

Section: :3mentioning

confidence: 99%

“…Potential biomarker in colorectal cancer . Linked to survival and invasion in lung cancer (Fujita et al 2015) Not reported Nie et al 2015, Lu et al 2016 and is upregulated in malignant prostate cancer as part of a glycosylation gene signature (Barfeld et al 2014a). As GALNT7 initiates O-glycosylation, upregulation of this enzyme could be linked to a range of changes to O-glycans in prostate cancer cells.…”

Section: :3mentioning

confidence: 99%

Glycosylation is a global target for androgen control in prostate cancer cells

Munkley¹

2017

Endocrine-Related Cancer

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Changes in glycan composition are common in cancer and can play important roles in all of the recognised hallmarks of cancer. We recently identified glycosylation as a global target for androgen control in prostate cancer cells and further defined a set of 8 glycosylation enzymes (GALNT7, ST6GalNAc1, GCNT1, UAP1, PGM3, CSGALNACT1, ST6GAL1 and EDEM3), which are also significantly upregulated in prostate cancer tissue. These 8 enzymes are under direct control of the androgen receptor (AR) and are linked to the synthesis of important cancer-associated glycans such as sialyl-Tn (sTn), sialyl LewisX (SLeX), O-GlcNAc and chondroitin sulfate. Glycosylation has a key role in many important biological processes in cancer including cell adhesion, migration, interactions with the cell matrix, immune surveillance, cell signalling and cellular metabolism. Our results suggest that alterations in patterns of glycosylation via androgen control might modify some or all of these processes in prostate cancer. The prostate is an abundant secretor of glycoproteins of all types, and alterations in glycans are, therefore, attractive as potential biomarkers and therapeutic targets. Emerging data on these often overlooked glycan modifications have the potential to improve risk stratification and therapeutic strategies in patients with prostate cancer.

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“…21 However, the role of miR-494-3p in NPC remains unclear, though there were studies reported the functional evidence of miR-494 in the tumorigenesis of NPC. 22 , 23 To better characterize how the altered expression of miR-494-3p might contribute to NPC development, we examined the expression levels of miR-494-3p in NPC and analyze its impacts on the biological functions of NPC cells.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-494-3p Promotes Cell Growth, Migration, and Invasion of Nasopharyngeal Carcinoma by Targeting Sox7

Liao

Yang

et al. 2018

Technol Cancer Res Treat

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Background:There is mounting evidence that microRNAs play an important role in nasopharyngeal carcinoma, which is widely prevalent in South China and is the most prevalent metastatic cancer among head and neck cancers. Recently, it has been shown that miR-494 is involved in the progression and prognosis of nasopharyngeal carcinoma. However, little is known about the function and mechanism of miR-494-3p in nasopharyngeal carcinoma. In the present study, we aimed to investigate the effects of miR-494-3p on the migration and invasion of nasopharyngeal carcinoma and to further explore the underlying mechanisms of these processes.Methods:The expression levels of miR-494-3p and Sox7 in nasopharyngeal carcinoma specimens and nasopharyngeal carcinoma cell lines were measured using quantitative reverse transcription polymerase chain reaction. Luciferase reporter assay, quantitative reverse transcription polymerase chain reaction, and Western blotting were used to confirm whether Sox7 was a direct target of miR-494-3p. Additionally, the roles of miR-494-3p and Sox7 on cell proliferation, migration, and invasion of nasopharyngeal carcinoma were analyzed by Cell Counting Kit-8 (CCK-8) assay, wound healing assay, and Boyden chamber assay, respectively.Results:Our study demonstrated that miR-494-3p was commonly upregulated in nasopharyngeal carcinoma specimens and nasopharyngeal carcinoma cell lines compared with nontumor nasopharyngeal epithelial tissue or nasopharyngeal cells (NP69). Moreover, miR-494-3p negatively regulated Sox7 at the posttranscriptional level by binding to a specific site in the Sox7 3′-untranslated region. In addition, synthetic miR-494-3p mimics significantly promoted proliferation, migration, and invasion of S18 and S26 nasopharyngeal carcinoma cells, while a synthetic miR-494-3p inhibitor resulted in suppressed nasopharyngeal carcinoma cell migration and invasion.Conclusion:miR-494-3p promotes nasopharyngeal carcinoma cell growth, migration, and invasion by directly targeting Sox7. Our results suggest that miR-494-3p might be a potential therapeutic target for nasopharyngeal carcinoma.

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GALNT7, a target of miR-494, participates in the oncogenesis of nasopharyngeal carcinoma

Cited by 17 publications

References 29 publications

MicroRNA-30e Functions as a Tumor Suppressor in Cervical Carcinoma Cells through Targeting GALNT7

MicroRNA-30e Functions as a Tumor Suppressor in Cervical Carcinoma Cells through Targeting GALNT7

Glycosylation is a global target for androgen control in prostate cancer cells

MicroRNA-494-3p Promotes Cell Growth, Migration, and Invasion of Nasopharyngeal Carcinoma by Targeting Sox7

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