Gambaran Histologi Regenerasi Hati Pasca Penghentian Pajanan Monosodium Glutamat pada Tikus Wistar

Andreas, Heryanto; Trianto, Heru Fajar; Ilmiawan, Muhammad In’am

doi:10.23886/ejki.3.4804.

Cited by 6 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The liver histopathological structure were assessed based on the scoring of the degree of liver damage in TABLE 1. 14 The score of the degree of liver damage of the control and the treatment group are shown in FIGURE 3. FIGURE 1 showed that the liver histopathology structure of both groups still normal.…”

Section: Resultsmentioning

confidence: 99%

“…The observation of the liver histopathological structure was conducted in 10 fields of view using a light microscope with 10x40 magnification to assess fat degeneration and necrosis, whilst 10×10 magnification to assess acini dilation.The degree of liver damage assessment was performed using the scoring for the degree of liver damage (TABLE 1). 14…”

Section: Histological Examinationmentioning

confidence: 99%

See 1 more Smart Citation

The Liver histopathology structure of Wistar rats on the acute toxicity test of Kapulaga Seeds extract (Amomum cardamomum)

Yudhani

Hendriyani²,

Pesik³

2020

IJPTher

View full text Add to dashboard Cite

One-third of the Indonesian population uses herbs for alternative medicine empirically. One of them is Kapulaga (Amomum cardamomum) which known as a spice and traditionally used for the treatment of rheumatic, fever, cough, sore throat, and mouth odor. The clinical use of herbs must be supported by scientific evidenced-based and its safety profile. The risk of exposure to a substance in humans can be identified through the toxicity tests on animals. The liver histopathological structure is one of the parameters of the acute toxicity test. This study to determine the acute toxicity of Kapulaga seeds extract (Amomum cardamomum) based on the liver histopathological structure of Wistar rats. The sample selection based on inclusion and exclusion criteria in the OECD 420 guideline. The research consisted of a preliminary and the main test. One rat was used in the preliminary test with an initial dose of cardamom seed extract 300 mg/kg BW and followed by a maximum dose of 2000 mg/kg BW after 48 hours. The main test used 10 rats that were randomly divided into control and treatment groups. Based on the result of the preliminary test, the treatment group was given a single dose of cardamom seed extract 2000mg /kg BW, whilst the control group was given distilled water. Observations of any toxic signs or dead were conducted every 30 minutes in the first 4 hours and continued once per day until 14thday. Assessment of the degree of liver damage was done using the liver damaged scoring and compared using the Mann Whitney test (α = 0.05). The average liver damage score of control and treatment groups is 0.96 ± 0,856 and 0.96 ± 0,604, respectively, and did not show any statistically significant differences (p>0,05). Kapulagaseeds (Amomum cardamomum) had no acute toxic effect based on the liver histopathological structure of Wistar rats.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Histological Examinationmentioning

confidence: 99%

The Liver histopathology structure of Wistar rats on the acute toxicity test of Kapulaga Seeds extract (Amomum cardamomum)

Yudhani

Hendriyani²,

Pesik³

2020

IJPTher

View full text Add to dashboard Cite

show abstract

“…Cells and hepatic tissue are tissues that have the ability to maintain cellular integrity from the effects of free radicals and free radical reaction chains formed during physiological stress (Andreas et al, 2015). It is further stated that the liver has an enzymatic and non-enzymatic system to neutralize or deactivate free radicals or free radical reaction chains involving the activity of several enzymes, including superoxide dismutase, glutathione, and catalase.…”

Section: Resultsmentioning

confidence: 99%

Application of Snakehead Fish to Increase Liver Function of Rats after Experiencing Physiological Stress

Sunarno¹,

Mardiati²,

Rahadian³

2018

J Bio Bio Edu

View full text Add to dashboard Cite

Physiological stress due to nutritional deficiency accompanied by excessive activity can cause problems of energy deficiency required by the body. Energy shortage can affect the body weight, as well as the weight and histology of the liver. This study was conducted to examine and analyze the effect of snakehead fish meat supplementation in feed toward body weight, weight and hepatic histology in animals experiencing physiological stress. This study used 20 male Wistar rats consisting of 5 treatments with 4 replications. The treatment in this study consisted of control (P0) and treatment (P1: 5%, P2: 10%, P3: 15%, P4: 20%) groups. This research used Completely Randomized Design (CRD). The variables measured in this study were body weight, hepatic weight, central venous diameter and hepatocytes in the test animals. The data obtained were analyzed using Analysis of Variance (ANOVA) at 5% significance level and continued with Duncan test with 5% significance. The result of data analysis showed that fish snakehead fish supplementation in feed gave a significant effect to body weight, weight and histology of animal liver. Concentrations of snakehead fish supplements can increase the body weight, hepatic weight, and hepatic histology which was indicated by higher central venous and hepatocyte diameter values compared to other controls and treatments. The conclusion of this research is the provision of snakehead fish supplement (Channia striata) can increase the body weight, hepatic weight, central venous and hepatocytes diameter of Wistar rats with the most influential concentration of 20%. This evidence is useful in studying the mechanism of liver repair due to stress

show abstract

“…Telah banyak penelitian yang melaporkan kerusakan berbagai organ akibat konsumsi MSG yang berlebih, di antaranya adalah hepar (Andreas, 2015). Hepar merupakan organ pusat dari metabolisme tubuh.…”

Section: Pendahuluanunclassified

Pengaruh Pemberian Ekstrak Tomat (Lycopersicon esculentum) Terhadap Gambaran HistopatologiI Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Wistar Yang Diinduksi Monosodium Glutamat (MSG)

Vidiyanti

2020

mul. AGRI-TEK. mul. ind. ilmu. ilmu. ind

View full text Add to dashboard Cite

ABSTRAKPENGARUH PEMBERIAN EKSTRAK TOMAT (Lycopersicon esculentum) TERHADAP GAMBARAN HISTOPATOLOGI HEPAR TIKUS PUTIH (Rattus norvegicus) JANTAN GALUR WISTAR YANG DIINDUKSI MONOSODIUM GLUTAMAT (MSG)Regga Arma VidiyantiMonosodium glutamat (MSG) adalah penambah rasa makanan yang telah banyak dikonsumsi masyarakat di seluruh dunia, tetapi bila dikonsumsi secara berlebih dapat menimbulkan kerusakan berbagai organ, diantaranya adalah hepar dikarenakan didalam hepar terjadi metabolisme obat dan toksikasi. Efek MSG dilaporkan dapat menyebabkan stress oksidatif. Efek toksik terhadap hepar dapat mengakibatkan terjadinya peroksidase lipid, serta perubahan morfologi jaringan hati yang ditandai dengan gangguan arsitektur hati, vakuolisasi, degenerasi, dan atrofi sel hati. Untuk mengantisipasi kerusakan hepar, diperlukan antioksidan. Salah satu sumber antioksidan adalah tomat.Penelitian ini bertujuan untuk mengetahui efek ekstrak tomat (Lycopersicon esculentum) terhadap gambaran histopatologi hepar tikus putih (Rattus norvegicus) jantan galur Wistar yang diinduksi MSG. Sampel yang digunakan sebanyak 24 ekor tikus putih (Rattus norvegicus) jantan galur Wistar yang dibagi menjadi 3 kelompok: (1) kelompok hewan coba yang diberi pakan standar; (2) kelompok hewan coba yang diberi MSG 5 mg/gBB/hari pada hari ke-1 s/d hari ke-14; dan (3) kelompok hewan coba yang diberi ekstrak tomat dosis 40 mg/kgBB dan MSG 5 mg/gBB/hari pada hari ke-1 s/d hari ke-14. Pada hari ke-15, hewan coba dikorbankan dan dilakukan pembuatan preparat hepar dan pemeriksaan gambaran mikroskopis. Uji analisis menggunakan Kruskal-Wallis dan Mann Whitney.Hasil uji Mann-Whitney menunjukkan perbedaan bermakna derajat kerusakan hepar pada kelompok hewan coba yang diberi pakan standar (rerata=1) dengan kelompok hewan coba yang diberi MSG (rerata=3,5) (p=0,001). Hasil uji Mann-Whitney menunjukkan perbedaan tidak bermakna derajat kerusakan hepar pada kelompok hewan coba yang diberi MSG (rerata=3,5) dengan kelompok hewan coba yang diberi MSG dan ekstrak tomat (rerata=3,125) (p=0,344).Kesimpulan dari hasil penelitian menunjukan bahwa MSG meningkatkan secara bermakna derajat kerusakan hepar yang digambarkan melalui gambaran histopatologi hepar dan ekstrak tomat (Lycopersicon esculentum) dapat menurunkan secara tidak bermakna derajat kerusakan hepar yang digambarkan melalui gambaran histopatologi hepar karena tomat memiliki flavonoid, vitamin C, fenol, likopen yang berfungsi sebagai antioksidan.Kata kunci : Monosodium glutamat, tomat (Lycopersicon esculentum), gambaran histopatologi hepar. ABSTRACTTHE EFFECT OF TOMATO EXTRACT (Lycopersicon esculentum) IN LIVER HISTOPATHOLOGICAL APPEARANCE ON WHITE RATS (Rattus norvegicus) WISTAR STRAIN MALE INDUCED BY MONOSODIUM GLUTAMAT (MSG)Regga Arma Vidiyanti Monosodium glutamate (MSG) is a food flavor enhancer that has been consumed by people all over the world, but if consumed excessively it can cause damage to various organs, including liver because drug metabolism and toxicity occur in the liver. The effect of MSG is reported to cause oxidative stress. Toxic effects on the liver can result in lipid peroxidase, as well as morphological changes in liver tissue characterized by impaired liver architecture, vacuolization, degeneration, and liver cell atrophy. To anticipate liver damage, antioxidants are needed. One of the source of antioxidants is tomatoes. This study aims to determine the effect of tomato extract (Lycopersicon esculentum) on the liver histopathology on white rats (Rattus norvegicus) Wistar strain male induced by MSG. The samples used were 24 white rats (Rattus norvegicus) Wistar strain male which were divided into 3 groups: (1) the experimental group of animals fed standard feed; (2) the experimental groups given MSG 5 mg / gBB / day on day 1 to day 14; and (3) the experimental group given a tomato extract dose of 40 mg / kgBB and MSG 5 mg / gBB / day on day 1 to day 14. On the 15th day, experimental animals were sacrificed and liver preparations were made and microscopic images were examined. The Test analysis of this study using Kruskal-Wallis and Mann Whitney. The results of the Mann-Whitney test showed that there is significant differences in the degree of liver damage in the experimental group given standard feed (mean = 1) with the experimental group given MSG (mean = 3.5) (p = 0.001). The Mann-Whitney test results showed there is no significant difference in the degree of liver damage in the experimental group given MSG (mean = 3.5) with the experimental group given MSG and tomato extract (mean = 3.125) (p = 0.344). The Conclusions of the results showed that MSG significantly increased the degree of liver damage described by liver histopathology and tomato extract (Lycopersicon esculentum) it can significantly reduce the degree of liver damage described by liver histopathology because tomatoes have flavonoids, vitamin C, phenol, Lycopene as an antioxidant. Keywords: Monosodium glutamate, tomatoes (Lycopersicon esculentum), liver histopathological appearance.

show abstract

Gambaran Histologi Regenerasi Hati Pasca Penghentian Pajanan Monosodium Glutamat pada Tikus Wistar

Cited by 6 publications

References 0 publications

The Liver histopathology structure of Wistar rats on the acute toxicity test of Kapulaga Seeds extract (Amomum cardamomum)

The Liver histopathology structure of Wistar rats on the acute toxicity test of Kapulaga Seeds extract (Amomum cardamomum)

Application of Snakehead Fish to Increase Liver Function of Rats after Experiencing Physiological Stress

Pengaruh Pemberian Ekstrak Tomat (Lycopersicon esculentum) Terhadap Gambaran HistopatologiI Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Wistar Yang Diinduksi Monosodium Glutamat (MSG)

Contact Info

Product

Resources

About