Influence of C-methyl-4,10,16,22-tetramethoxy calix[4]arene compound as inhibitor of calcium sulphate scale formation has been studied using seeded experiment method. The results show that C-methyl-4,10,16,22-tetramethoxy calix[4]arene as calix[4]arene derived compound inhibits a formation of calcium sulphate crystals at a concentration of growth solution of 0.1 M and at various inhibitor concentrations of 0-150 ppm. The ability of C-methyl-4,10,16,22-tetramethoxy calix[4]arene as inhibitor of the formation of calcium sulphate scale is optimum in inhibiting growth rate of CaSO4 scale formation is 50 ppm at 80 ºC.