Gametophytic phase of "Doryopteris triphylla" (Pteridaceae, Polypodiopsida).

Abstract

Gametophytes of the Pteridaceae are moderately well known but there is still a quite large set of species to describe. Among these is Doryopteris triphylla, a member of the cheilanthoid clade (subgroup hemionitids). The main objective of this work is to describe the gametophyte of D. triphylla. In vitro cultures were prepared with spores coming from various sporophytes of different locations. In vivo observations were done periodically to monitor developmental critical events and reproductive activity. Germination followed the Vittaria pattern and the developmental processes adjusted to the Adiantum type. Adult cordate gametophytes produced normal sexual organs in unisexual prothalli. Doryopteris triphylla gametophytes exhibited somewhat peculiar features: the apical cell divided longitudinally, the first meristematic cell was rectangular, and archegoniate prothalli appeared much before than antheridiate ones.

Study of the Gametophytic phase of Diplazium caudatum (Athyriaceae, Polypodiopsida) from Spain

“…They were extracted by exposing the fronds to a mild heat for one night. The cultivation protocol follows the guidelines of other similar works (Gabriel y Galán et al 2008;Seral et al 2016), with some modifications regarding the photoperiod. The spores were mixed to avoid possible individual differences.…”