In this study, a prototype of an inertial sensor-based gait sonification system was tested for the purpose of providing real-time gait feedback on the knee angle. The study consisted of two parts: (1) a comparison of the knee angle measurement to a marker-based 3D optical capturing system (Vicon, Oxford, UK) with N = 24 participants and (2) an evaluation four different sonification feedback versions in an accentuation × pitch (2 × 2) design on a sample of N = 28 participants. For the measurement system comparison, the RMSE was 7.6° ± 2.6° for the left and 6.9° ± 3.1° for the right side. Measurement agreement with bias up to −7.5° ± 6.2° (for maximum knee flexion) was indicated by the Bland–Altmann Method. The SPM revealed significant differences between both measurement systems for the area 45–90% (p < 0.001) (left) and the area between 45% and 80% (p = 0.007) (right). For the sonification perception, the variation of pitch had a significant effect on the perception of pleasantness of the sound. No effect was found for the accentuation of the swing or stance phase.